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... important aspects of sexual biology. First, we have little knowledge of the molecular links between dsx and the biological processes it controls. Second, understanding how the functions of the sex hierarchy and other patterning hierarchies are integrated during development requires the identificatio ...
Bioinformatics
Bioinformatics

... – # tr (translate first set into second; e.g. g becomes c ) complement (replace) ...
BioACTS Quarter THREE
BioACTS Quarter THREE

... VB— Genetics: The scientific study of heredity. Meiosis: Process by which the number of chromosomes per cell is cut in half through the separation of homologous chromosomes in a diploid cell. Same—genetic information to form life. ...
The Effects of varying pH on Plasmid Transfer
The Effects of varying pH on Plasmid Transfer

... only a single labeled strand is transferred. The result of this process, if uninterrupted, is that both the donor and recipient possess completely formed plasmids. The entire process of conjugation is highly efficient. Virtually every recipient that comes into contact with a donor receives the donor ...
Driscoll Katee Driscoll Dr. Ely Genetics October 20, 2013 Effects of
Driscoll Katee Driscoll Dr. Ely Genetics October 20, 2013 Effects of

... optimal prediction model, which relied on the method of splitting the data (using some of the data as a training set to predict the HTT CAG repeat lengths for the remaining samples that comprised the other set) (Lee et al. 2013). When the model was run, it was found to predict CAG repeat lengths wit ...
Analysis of a Rhizobium leguminosarum gene
Analysis of a Rhizobium leguminosarum gene

... The deduced polypeptide product of gstA has a calculated molecular mass of 21989 Da. A database search of its predicted amino acid sequence showed it to be homologous to a number of GST or GST-like sequences from plants, animals and other bacteria (Fig. 2). The similarity in sequence is most strikin ...
Temperate and lytic bacteriophages programmed to sensitize and
Temperate and lytic bacteriophages programmed to sensitize and

... sensitized pathogens would most likely fail due to escape mutants that are selected by the antibiotics. Here we demonstrate a strategy to counteract the emerging threat of antibiotic-resistant bacteria that evades the above shortcomings. Instead of directly killing the pathogens, we propose to sensi ...
Mapping the histone code at hMLH1. - JScholarship
Mapping the histone code at hMLH1. - JScholarship

... Laird, 1999). Two models have been proposed for the mechanism of DNA hypermethylation associated silencing. The first model suggests that DNA hypermethylation directly prevents sequence specific transcription factors from binding to the DNA (Robertson and Jones, 2000). The second model proposes that ...
Multistep Small-Molecule Synthesis Programmed by
Multistep Small-Molecule Synthesis Programmed by

... strategy, cleavage generates a “useful scar” that can be functionalized in subsequent steps. As an example of this class of linker, we generated amino acid reagents such as L-Phe derivative 5 linked through 1,2-diols4d to their decoding DNA oligonucleotides. Following DNA-templated amide bond format ...
Isolation and characterization of a repeated sequence (RPS1) of
Isolation and characterization of a repeated sequence (RPS1) of

Accepted Version - CSIRO Research Publications Repository
Accepted Version - CSIRO Research Publications Repository

... Received: 30 May 2012; in revised form: 27 July 2012 / Accepted: 30 July 2012 / Published: 8 August 2012 ...
Chapter 14 - People Server at UNCW
Chapter 14 - People Server at UNCW

... Copyright © McGraw-Hill Education. Permission required for reproduction or display. ...
High throughput nucleic acid sample preparation in 96 well plates
High throughput nucleic acid sample preparation in 96 well plates

... qRT-PCR of Norovirus genogroup I (FAM channel (A)) and Norovirus genogroup II (ROX channel (B)). Amplification plots of normalized fluorescence versus the PCR cycle numbers are displayed for manually extracted RNA specimens (red plots) and automatically extracted RNA specimens using the Invisorb® Vi ...
chapter 3 transmission genetics – chromosomes, recombination and
chapter 3 transmission genetics – chromosomes, recombination and

... in Mendel’s peas there was one allele coding for tall plants (D) and one allele coding for short plants (d) and these alleles segregated among the offspring. Plants that have the same allele at a locus on each of the homologous chromosomes are homozygous (e.g. DD and dd), whereas those with a differ ...
PDF
PDF

... Waddington (1905–1975), who derived the term from the Aristotelian word epigenesis. There exists some controversy around the word epigenetics and its broad definition. It includes any modification of the expression of genes due to factors other than mutation in the DNA sequence. This involves DNA me ...
DNA Sequence Capture and Enrichment by Microarray Followed by
DNA Sequence Capture and Enrichment by Microarray Followed by

unit-2 genetics of prokaryotes and eukaryotic
unit-2 genetics of prokaryotes and eukaryotic

... chromosome or for finding out distances of these genes from centromere, this technique is called chromosome mapping. If linkage groups are already established in an organism, trisomics can be effectively used for assigning these linkage groups to specific chromosomes. Since the segregation ratio for ...
Transposon stability and a role for conjugational transfer in adaptive mutability
Transposon stability and a role for conjugational transfer in adaptive mutability

... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
Meiosis/Crossing Over - Peoria Public Schools
Meiosis/Crossing Over - Peoria Public Schools

... genes in a gamete has no influence over which allele of another gene is present.” Random Orientation refers to the behaviour of homologous pairs of chromosomes (metaphase I) or pairs of sister chromatids (metaphase II) in meiosis. Independent assortment refers to the behaviour of alleles of unlinked ...
S - www2
S - www2

... Because of the size of the tubes (several ml), one of the major problems to obtain quantitative information on sedimentation velocity from a preparative ultracentrifuge is the disturbance of the material distribution by turbulence from physical vibrations or due to convection from thermal differenc ...
- Wiley Online Library
- Wiley Online Library

... OD550 nm of 0.15–0.2. However, after c. 30 min of competence, the ability to take up DNA is rapidly lost (Tomasz, 1966). It was reported 35 years ago that cell-free supernatants from various competent streptococcal cultures contained a proteinaceous compound that could induce competence in noncompet ...
Analysis of the mitochondrial COI gene and its
Analysis of the mitochondrial COI gene and its

... markers used in molecular systematics. Portions of this gene are often used to infer phylogenies. In addition, COI is currently the focus of considerable interest, especially its 5' portion, which is used by the DNA Barcoding Consortium (Hebert et al., 2003; Stoeckle, 2003). This region is comprised ...
Canadian Journal of Microbiology
Canadian Journal of Microbiology

... published yet that report the application of 16S rDNA based community fingerprint techniques, such as denaturing gradient gel electrophoresis (Araújo et al. 2002; Garbeva et al. 2001; Sessitsch et al. 2002) and terminal restriction length polymorphism analysis (T-RFLP) (Krechel et al. 2002; Reiter e ...
22 P.3d 68 - Daubert Tracker
22 P.3d 68 - Daubert Tracker

... The D1S80 test is a hybrid of the PCR and RFLP methods. It detects fragment length polymorphisms once the DNA fragment has been amplified through the PCR procedure. Another form of PCR testing involves the use of locations on the DNA strand containing short tandem repeats ("STR") of baseline pattern ...
Transcription - Shippensburg University
Transcription - Shippensburg University

... The Genetic Code • How are the instructions for assembling amino acids into proteins encoded into DNA? • There are 20 amino acids, but there are only four nucleotide bases in DNA ...
< 1 ... 38 39 40 41 42 43 44 45 46 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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