Transposition - Pennsylvania State University
... one location in the genome to another • Found in all organisms (so far studied) • Effects: – Insertion near or within a gene can inactivate or activate the target gene. – Cause deletions, inversions, and translocations of DNA – Lead to chromosome breaks ...
... one location in the genome to another • Found in all organisms (so far studied) • Effects: – Insertion near or within a gene can inactivate or activate the target gene. – Cause deletions, inversions, and translocations of DNA – Lead to chromosome breaks ...
Microscopes
... This study guide packet is due the day of the final exam. You must complete it and bring it with you when you come to take the test. If you do not have the study guide completed and in class at the beginning of class on the day of your final, you will not get any credit for it. This is an “all or no ...
... This study guide packet is due the day of the final exam. You must complete it and bring it with you when you come to take the test. If you do not have the study guide completed and in class at the beginning of class on the day of your final, you will not get any credit for it. This is an “all or no ...
Objectives 25
... proto-oncogene function (viral oncogene src gene for sarcoma virus, but no cellular homolog to this viral gene product found) - cellular homolog to avian MC29 myelocytomatosis virus myc oncogene found - proto-oncogenes usually involved in proliferation regulation; most identified by homology to vi ...
... proto-oncogene function (viral oncogene src gene for sarcoma virus, but no cellular homolog to this viral gene product found) - cellular homolog to avian MC29 myelocytomatosis virus myc oncogene found - proto-oncogenes usually involved in proliferation regulation; most identified by homology to vi ...
HYS2, an essential gene required for DNA replication in
... which we are calling hysl-hys4 (Table 2). In this paper, we present data characterizing the hys2 mutation. The HU-treated cultures of hys2-l mutant were subjected to cytological examination. Cells were stained with the DNA specific fluorescence dye DAPI for analysis of cell and nuclear morphology. M ...
... which we are calling hysl-hys4 (Table 2). In this paper, we present data characterizing the hys2 mutation. The HU-treated cultures of hys2-l mutant were subjected to cytological examination. Cells were stained with the DNA specific fluorescence dye DAPI for analysis of cell and nuclear morphology. M ...
BIOLOGY
... Although humans have been manipulating organisms for millennia, genetic engineering simplifies and targets manipulations in an unprecedented way. Transgenic plants and animals are generated with characteristics that cannot be obtained using traditional breeding. Unlike organisms generated by selecti ...
... Although humans have been manipulating organisms for millennia, genetic engineering simplifies and targets manipulations in an unprecedented way. Transgenic plants and animals are generated with characteristics that cannot be obtained using traditional breeding. Unlike organisms generated by selecti ...
GLP 021 - University of Newcastle
... Risk Assessment: This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this equipment. Therefore, personnel are requested to conduct their own Risk Assessment before using this equipment to include any extra h ...
... Risk Assessment: This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this equipment. Therefore, personnel are requested to conduct their own Risk Assessment before using this equipment to include any extra h ...
DNA methylation
... produce a phenotype. Robin Holliday defined epigenetics as "the study of the mechanisms of temporal and spatial control of gene activity during the development of complex organisms.“ Thus epigenetic can be used to describe anything other than DNA sequence that influences the development of an organi ...
... produce a phenotype. Robin Holliday defined epigenetics as "the study of the mechanisms of temporal and spatial control of gene activity during the development of complex organisms.“ Thus epigenetic can be used to describe anything other than DNA sequence that influences the development of an organi ...
Select one of your Biology instructors from another class and look
... which DNA samples are placed ("loaded") in the depressions ("wells") at the top of the gel and electrophoresis is in the downward direction. The dashed lines on the right denote the positions to which DNA fragments of various sizes would migrate. The fragment sizes are given in kilobase pairs (kb); ...
... which DNA samples are placed ("loaded") in the depressions ("wells") at the top of the gel and electrophoresis is in the downward direction. The dashed lines on the right denote the positions to which DNA fragments of various sizes would migrate. The fragment sizes are given in kilobase pairs (kb); ...
Recombinant DNA Technology
... short peptide called an epitope, for which very specific anitbodies exist. It also gets fused to 6 histidines, which allow easy purification on a column that has nickel ions bound to it (an affinity tag). For growth in mammalian cells, it has an SV40 viral origin of replication (SV40ori), and a zeoc ...
... short peptide called an epitope, for which very specific anitbodies exist. It also gets fused to 6 histidines, which allow easy purification on a column that has nickel ions bound to it (an affinity tag). For growth in mammalian cells, it has an SV40 viral origin of replication (SV40ori), and a zeoc ...
Cells in culture.
... Knowledge of the DNA sequence to be amplified is used to design two synthetic DNA oligonucleotides, each complementary to the sequence on one strand of the DNA double helix at opposite ends of the region to be amplified. These oligonucleotides serve as primers for in vitro DNA synthesis, which is pe ...
... Knowledge of the DNA sequence to be amplified is used to design two synthetic DNA oligonucleotides, each complementary to the sequence on one strand of the DNA double helix at opposite ends of the region to be amplified. These oligonucleotides serve as primers for in vitro DNA synthesis, which is pe ...
comparative genomics
... Orthologues are homologues that have evolved from a common ancestral gene by speciation. They usually have similar functions. Paralogues are homologues that are related or produced by duplication within a genome followed by subsequent divergence. They often have different functions. Xenologues are h ...
... Orthologues are homologues that have evolved from a common ancestral gene by speciation. They usually have similar functions. Paralogues are homologues that are related or produced by duplication within a genome followed by subsequent divergence. They often have different functions. Xenologues are h ...
Chemistry 100 Exam 3 Part 2
... Discuss 2 ways your body can respond during sun exposure. 1. UV damage causes SUNBURN. Immune system is called into action to clean up dead cells and kill cells that are too damaged to repair. Blood vessels dilate to bring more blood to skin's surface, causing heat and redness. 2. UV causes TAN, an ...
... Discuss 2 ways your body can respond during sun exposure. 1. UV damage causes SUNBURN. Immune system is called into action to clean up dead cells and kill cells that are too damaged to repair. Blood vessels dilate to bring more blood to skin's surface, causing heat and redness. 2. UV causes TAN, an ...
Short read alignment, genome alignment, and high performance
... Short read alignment • Input: – Reads: short DNA sequences usually up to 100 base pairs (bp) produced by a sequencing machine • Reads are fragments of a longer DNA sequence present in the sample given as input to the machine • Usually number in the millions ...
... Short read alignment • Input: – Reads: short DNA sequences usually up to 100 base pairs (bp) produced by a sequencing machine • Reads are fragments of a longer DNA sequence present in the sample given as input to the machine • Usually number in the millions ...
general biology final exam review guide
... Be able to describe Gregor Mendel’s contributions to science Be able to describe a gene. alleles, phenotype and genotype. ...
... Be able to describe Gregor Mendel’s contributions to science Be able to describe a gene. alleles, phenotype and genotype. ...
PlayMais 3-D DNA Model
... ● Take a yellow flake (deoxyribose), a blue flake (phosphate group), and a colored flake (nucleobase). Using a damp sponge, wet one end of a blue flake. Stick the wet end of the blue flake onto one end of a yellow flake by gently pushing them together. Keep them in position with your hands for appro ...
... ● Take a yellow flake (deoxyribose), a blue flake (phosphate group), and a colored flake (nucleobase). Using a damp sponge, wet one end of a blue flake. Stick the wet end of the blue flake onto one end of a yellow flake by gently pushing them together. Keep them in position with your hands for appro ...
Restriction enzymes
... • These enzymes protect bacteria against intruding DNA from other organisms. • They work by cutting up the foreign DNA, a process called restriction. • If foreign DNA enters the bacteria cell the restriction enzyme will cut it up into small pieces. They cut up only certain base pair sequences and th ...
... • These enzymes protect bacteria against intruding DNA from other organisms. • They work by cutting up the foreign DNA, a process called restriction. • If foreign DNA enters the bacteria cell the restriction enzyme will cut it up into small pieces. They cut up only certain base pair sequences and th ...
Decoding the Gene - Warren Hills Regional School District
... Notice AUG specifies methionine, or the “Start” codon & there are three “Stop” codons to specify the end of a protein. ...
... Notice AUG specifies methionine, or the “Start” codon & there are three “Stop” codons to specify the end of a protein. ...
Extraction of Plasmid DNA, Restriction Digest, and DNA Gel
... We then insert this plasmid that now contains the gene that codes for protein X into E. coli cells. The machinery inside the cells makes large amounts of protein X based on the sequence of the gene. This is called overexpression. The cornerstone of this technology is the plasmid. Plasmids are circul ...
... We then insert this plasmid that now contains the gene that codes for protein X into E. coli cells. The machinery inside the cells makes large amounts of protein X based on the sequence of the gene. This is called overexpression. The cornerstone of this technology is the plasmid. Plasmids are circul ...
transformation
... a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of human insulin by bacteria. In this activity, students will model how DNA transformation work ...
... a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of human insulin by bacteria. In this activity, students will model how DNA transformation work ...
Regulation of Gene Activity in Eukaryotes
... • Supplying DNA and RNA sequences as research tools. • Altering the genotype of organisms (both plants and animals) (transgenesis) • Potentially correcting genetic defects in animals (gene therapy) ...
... • Supplying DNA and RNA sequences as research tools. • Altering the genotype of organisms (both plants and animals) (transgenesis) • Potentially correcting genetic defects in animals (gene therapy) ...
STUDY GUIDE for Dr. Mohnen`s part of Exam #3
... Enhancer: cis-acting element that can be on either DNA strand and stimulate transcription even 1000s of bp away Most eukaryote TFs interact with multiple proteins to give large complexes Mediator: complex of 25-30 subunits interact with transcription machinery before initiation begins; bridge betwee ...
... Enhancer: cis-acting element that can be on either DNA strand and stimulate transcription even 1000s of bp away Most eukaryote TFs interact with multiple proteins to give large complexes Mediator: complex of 25-30 subunits interact with transcription machinery before initiation begins; bridge betwee ...
Genetic Manipulation of Bacteria
... If the vector is a plasmid that uses a replication mechanism with a single stranded intermediate like the strand-displacement mechanism of IncQ plasmids or the rolling circle replication of many Gram-positive plasmids then the instability is promoted by increasing the size of the single stranded seg ...
... If the vector is a plasmid that uses a replication mechanism with a single stranded intermediate like the strand-displacement mechanism of IncQ plasmids or the rolling circle replication of many Gram-positive plasmids then the instability is promoted by increasing the size of the single stranded seg ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.