Introduction to Vectors - Pascack Valley Regional School District
... There are three commonly used types of vectors: 1) plasmid vectors (e.g., pUC plasmids); 2) bacteriophage vectors (e.g., phage ); and 3) phagemid vectors (e.g., pBlueScriptTM). Each has a different use, and there are many derivatives of these basic building blocks. In BRITE, you will be using plasm ...
... There are three commonly used types of vectors: 1) plasmid vectors (e.g., pUC plasmids); 2) bacteriophage vectors (e.g., phage ); and 3) phagemid vectors (e.g., pBlueScriptTM). Each has a different use, and there are many derivatives of these basic building blocks. In BRITE, you will be using plasm ...
Lecture #6 Date ______ - Pomp
... effects on offspring, depending on whether they arrive in the zygote via the ovum or via the sperm. • Fragile X syndrome: higher prevalence of disorder and retardation in males ...
... effects on offspring, depending on whether they arrive in the zygote via the ovum or via the sperm. • Fragile X syndrome: higher prevalence of disorder and retardation in males ...
Prentice Hall Review PPT. Ch. 12
... code? Why or why not? How do the proteins made affect the type and function of cells? Cells do not make all of the proteins for which they have genes (DNA). The structure and function of each cell are determined by the types of proteins present. 2. Consider what you now know about genes and protein ...
... code? Why or why not? How do the proteins made affect the type and function of cells? Cells do not make all of the proteins for which they have genes (DNA). The structure and function of each cell are determined by the types of proteins present. 2. Consider what you now know about genes and protein ...
Lynch Syndrome
... The human body is made of small living units called cells. Cells in the body contain millions of genes. Genes are the basic structural and functional units of heredity. ...
... The human body is made of small living units called cells. Cells in the body contain millions of genes. Genes are the basic structural and functional units of heredity. ...
August 2007
... M. jalapa plants with deep crimson flowers and M. jalapa plants with yellow flowers were studied. Cross-pollinating these plants produced plants with deep red flowers only (F1 generation). These F1 plants were allowed to self-pollinate, and the resulting seeds produced 450 deep red and 160 yellow M. ...
... M. jalapa plants with deep crimson flowers and M. jalapa plants with yellow flowers were studied. Cross-pollinating these plants produced plants with deep red flowers only (F1 generation). These F1 plants were allowed to self-pollinate, and the resulting seeds produced 450 deep red and 160 yellow M. ...
8 Hershey and Chase Experiment
... Proteins contain sulfur, and not phosphorous When the Hershey-Chase experiment tested the proteins for hereditary action, their replicates were not radioactive ...
... Proteins contain sulfur, and not phosphorous When the Hershey-Chase experiment tested the proteins for hereditary action, their replicates were not radioactive ...
DNA Analysis
... – Much of the DNA is non-coding (junk DNA) and even in protein coding genes, there may be sequences that are cut out (introns) before they are used to make a protein. The remaining sequences are the exons. – Genes are sequences of DNA – there are only 4 building blocks of DNA (A,T,G and C), so the g ...
... – Much of the DNA is non-coding (junk DNA) and even in protein coding genes, there may be sequences that are cut out (introns) before they are used to make a protein. The remaining sequences are the exons. – Genes are sequences of DNA – there are only 4 building blocks of DNA (A,T,G and C), so the g ...
Unit 5 Review
... Name one thing that DNA provides templates for Name two of the three important roles of cell division True or false: Binary Fission produces two genetically unique cells Name the process by which single-celled eukaryotic organisms produce genetically identical copies of themselves How many daughter ...
... Name one thing that DNA provides templates for Name two of the three important roles of cell division True or false: Binary Fission produces two genetically unique cells Name the process by which single-celled eukaryotic organisms produce genetically identical copies of themselves How many daughter ...
Activity 4.5: Forensic DNA Fingerprinting
... When setting up restriction digests use fresh tips each time to prevent contamination Tubes can be incubated in a water bath, dry bath, or at room temperature overnight – If incubating overnight, it is helpful to incubate for a short while at 37ºC first, then let come to room temperature overnig ...
... When setting up restriction digests use fresh tips each time to prevent contamination Tubes can be incubated in a water bath, dry bath, or at room temperature overnight – If incubating overnight, it is helpful to incubate for a short while at 37ºC first, then let come to room temperature overnig ...
UNIT 1: Biology as the Science of Life
... diagram the double helix model of DNA. demonstrate the replication of DNA. discuss the evidence for the fact that DNA codes for proteins. demonstrate the events of transcription and translation. explain the connection between the proteins produced by translation and the traits possessed by ...
... diagram the double helix model of DNA. demonstrate the replication of DNA. discuss the evidence for the fact that DNA codes for proteins. demonstrate the events of transcription and translation. explain the connection between the proteins produced by translation and the traits possessed by ...
supplementary materials
... earlier ORFs used the microarray experiments done by Chu et al [8] are not included in the sequence files. In the set of the middle genes identified by using the expression data at 2 and 5 hours, the following ORFs are not included in the S. cerevisiae sequence file: ...
... earlier ORFs used the microarray experiments done by Chu et al [8] are not included in the sequence files. In the set of the middle genes identified by using the expression data at 2 and 5 hours, the following ORFs are not included in the S. cerevisiae sequence file: ...
Genetic mapping RFLP: Restriction Fragment Length
... – Find the difference between the RFLP markers of family members with the disease and family members not having the disease. – It is likely that the RFLP marker that consistently differ is on the gene responsible for the disease, since family members have more or less the same genetic characteristic ...
... – Find the difference between the RFLP markers of family members with the disease and family members not having the disease. – It is likely that the RFLP marker that consistently differ is on the gene responsible for the disease, since family members have more or less the same genetic characteristic ...
Algorithms for Bioinformatics Autumn 2010
... PSSM is a log-odds normalized version of PWM. 1 Calculated by log(pai/qa), where pai is the frequency of a at column i in the samples. qa is the probability of a in the whole organism (or in some region of interest). Problematic when some values pai are zero. Solution is to use pseudocou ...
... PSSM is a log-odds normalized version of PWM. 1 Calculated by log(pai/qa), where pai is the frequency of a at column i in the samples. qa is the probability of a in the whole organism (or in some region of interest). Problematic when some values pai are zero. Solution is to use pseudocou ...
MUTATIONS
... • May lead to amino acid change – See animation • May not lead to any change (Silent Mutation) – Ex: DNA “CCC” is mutated into “CCG” » Same amino acid is created (glycine) ...
... • May lead to amino acid change – See animation • May not lead to any change (Silent Mutation) – Ex: DNA “CCC” is mutated into “CCG” » Same amino acid is created (glycine) ...
BCM301 Food Biotechnology
... DNA, and why they are important in biotechnology. Compare blunt ends with sticky ends. Know the mechanism by which electrophoresis separates pieces of DNA. List and know the steps of DNA cloning. Know how vectors are used to transform bacteria, and know the methods of selecting for successfully tran ...
... DNA, and why they are important in biotechnology. Compare blunt ends with sticky ends. Know the mechanism by which electrophoresis separates pieces of DNA. List and know the steps of DNA cloning. Know how vectors are used to transform bacteria, and know the methods of selecting for successfully tran ...
Lecture 2
... F.Watson and J.Crick gathered all available data in an attempt to develop a model of DNA structure. The data known at the time was that DNA was a long molecule, proteins were helically coiled (as determined by the work of Linus Pauling), Chargaff's base data, and the X-ray diffraction data of R.Fran ...
... F.Watson and J.Crick gathered all available data in an attempt to develop a model of DNA structure. The data known at the time was that DNA was a long molecule, proteins were helically coiled (as determined by the work of Linus Pauling), Chargaff's base data, and the X-ray diffraction data of R.Fran ...
5.1.1 Cellular Control
... A research team was investigating the properties of a newly-discovered enzyme, the product of which was a valuable drug. This enzyme had been extracted from cells of a marine worm, found in the North Atlantic, where the temperature is always close to 5 °C. All the proteins of such animals are adapte ...
... A research team was investigating the properties of a newly-discovered enzyme, the product of which was a valuable drug. This enzyme had been extracted from cells of a marine worm, found in the North Atlantic, where the temperature is always close to 5 °C. All the proteins of such animals are adapte ...
Sickle cell / mutations
... 2. Unlike popular misconceptions about people with green skin or extra body parts, a mutation is simply a change in the nucleotide sequence, or base pair sequence, of DNA. Most mutations are either neutral (they have no effect) or harmful, but occasionally mutations can actually cause a helpful chan ...
... 2. Unlike popular misconceptions about people with green skin or extra body parts, a mutation is simply a change in the nucleotide sequence, or base pair sequence, of DNA. Most mutations are either neutral (they have no effect) or harmful, but occasionally mutations can actually cause a helpful chan ...
Lecture 22: Protein Engineering
... Lecture 22: Protein Engineering Proteins have important role in physiological processes and they are involved in movement, catalysis, recognition, regulation etc. Moreover, proteins also have several therapeutical and industrial applications. Advances in Molecular Biology have enabled us to manipula ...
... Lecture 22: Protein Engineering Proteins have important role in physiological processes and they are involved in movement, catalysis, recognition, regulation etc. Moreover, proteins also have several therapeutical and industrial applications. Advances in Molecular Biology have enabled us to manipula ...
Document
... • Plasmid – small, circular, self-replicating piece of DNA – R plasmids – special – genes inserted ...
... • Plasmid – small, circular, self-replicating piece of DNA – R plasmids – special – genes inserted ...
TOPIC: Applied Genetics AIM: What methods can be used to
... years. Recently, though, Frank is showing signs of old age, and you realize that your friend's days are numbered. You can't bear the thought of living without her, so you contact a biotechnology company that advertises pet cloning services. For a fee, this company will clone Frank using DNA from a s ...
... years. Recently, though, Frank is showing signs of old age, and you realize that your friend's days are numbered. You can't bear the thought of living without her, so you contact a biotechnology company that advertises pet cloning services. For a fee, this company will clone Frank using DNA from a s ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.