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Gene Expression
Gene Expression

... proteins. A recipe tells you what order to apply the ingredients and how to mix them. Some of these junk DNA segments do have a function: to regulate the gene expression of other genes. There are proteins whose job it is to monitor the cell size, and when the cell gets too big, they race back to the ...
Document
Document

Basic Principles of Human Genetics
Basic Principles of Human Genetics

... Eddy is a 4-year-old boy brought in by his parents because of recurrent cough. He has had two bouts of pneumonia, which were treated with antibiotics, over the past 2 months. Now he is sick again, having never stopped coughing since the last episode of pneumonia. He has also been noted by his parent ...
Document
Document

... • Only 1/10,000 cells transfer the complete chromosome, including the remainder of the F factor, • Thus, most recipient cells remain F-. ...
Chapter 1
Chapter 1

... chains if provided with a template and nucleotide precursors but can not initiate DNA replication in the absence of a primer. Only RNA polymerases have this ability. A RNA polymerase called primase is indeed fulfilling this role on the lagging strand. The prima ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • Hybridization is the ability of one singlestranded nucleic acid to form a double helix with another single strand of complementary base sequence • Previous discussion focused on colony and plaque hybridization • This section looks at techniques for ...
No Slide Title
No Slide Title

... Advantage - massive coverage of sequences possible Disadvantage - expensive and not easily customized ...
3 macromolecules no pics pdf
3 macromolecules no pics pdf

... carrying molecule; it is also structurally made to replicate itself – Through simple base pairing, DNA basically has two copies at all time – All DNA polymerase (the protein that helps form the new DNA strand) has to do is unzip the molecule • Once the molecule is unzipped, deoxyribose nucleotides w ...
Biohazardous Materials/rDNA Summary Form
Biohazardous Materials/rDNA Summary Form

EZ-DNA - Geneflow
EZ-DNA - Geneflow

Patterns of Inheritance DNA Chromosome(s) Gene(s) Character(s
Patterns of Inheritance DNA Chromosome(s) Gene(s) Character(s

... • Mendelian Patterns: Based on research and experimentation of simple organisms by Gregor Mendel. • Non-Mendelian Patterns: Based on research and experimentation since Mendel. Compilation of knowledge of more complex organisms since his time. ...
chapt13_image
chapt13_image

... binding proteins that speed transcription • They bind to a region of DNA called enhancer that can be far away from promoter • A hairpin loop in the DNA brings the transcription activators attached to enhancers into contact with transcriptional factor complex • Transcription factors, activators,and r ...
Support worksheet – Chapter 4 - Cambridge Resources for the IB
Support worksheet – Chapter 4 - Cambridge Resources for the IB

... Assume that in humans the allele for brown eyes (B) is dominant over the allele for blue eyes (b). A blue-eyed man, both of whose parents had brown eyes, marries a brown-eyed woman. They have one child who has blue eyes. What are the genotypes of the man, his parents, his wife and the child? ...
CH. 13 - Weebly
CH. 13 - Weebly

... • Transfer RNA: carries amino acids to ribosome and matches them to the coded mRNA ...
Eukaryotic transcriptional control
Eukaryotic transcriptional control

... monomers form a coiled-coil dimer. Basic amino acid residues N-terminal to the leucine zipper form the DNA-binding domain. ...
Sexual reproduction
Sexual reproduction

... contributed from two different members of the species ...
Mendel`s Laws Haldane`s Mapping Formula
Mendel`s Laws Haldane`s Mapping Formula

... • If there is an even number of crossovers between two sites, they wind up on the same gamete. The net effect is no recombination. • If there is an odd number of crossovers between sites, they recombine. • AB = event “recombination between A & B” = “odd # of crossovers between A & B” ...
brief talk
brief talk

... Lauria, Mario, Kaustubh Bhalerao, Muthu M. Pugalanthiran, and Bo Yuan. “Building blocks of a biochemical CPU based on DNA transcription logic.” 3rd Workshop on Non-Silicon Computation (NSC-3), Munich, June 2004. Molecular Beacons: A Novel DNA Probe for Nucleic Acid and Protein ...
Chapter 17~ From Gene to Protein
Chapter 17~ From Gene to Protein

...  How do we move information from DNA to proteins? ...
A Picture`s Worth 1000 Words INTRODUCTION DNA fingerprinting
A Picture`s Worth 1000 Words INTRODUCTION DNA fingerprinting

... There is another common and valuable use of this technology, and this involves diagnosis of diseases. If a genetic disease runs in a particular family, the DNA from each member of the family can be analyzed and a pattern may emerge. For example, if every individual in a family that is affected by a ...
File - thebiotutor.com
File - thebiotutor.com

... The better responses included extraction of the gene, that codes for growth hormone, from human DNA using a restriction enzyme. Using the same restriction enzyme to cut a bacterial plasmid and using ligase to insert the human gene into the plasmid to make a vector contain recombinant DNA. In part (b ...
ppt
ppt

Organic Compounds
Organic Compounds

... doesn’t have chloroplasts because they are prokaryotes) - chloroplasts have a inner membrane known as the thylakoid membrane and the space between the thylakoid membrane and the inner membrane is called a stroma (fluid filled) - chloroplasts are found in mesophyl cells - the visible light spectrum i ...
Biology Keystone Review Packet Module 2 with Answers
Biology Keystone Review Packet Module 2 with Answers

... a. In order for a male offspring to be a hemophiliac, his mother must be a hemophiliac. b. In order for a female offspring to be a hemophiliac, her father must be a hemophiliac. c. In order for a male offspring to be a hemophiliac, his father must be a hemophiliac. d. In order for a female offspring ...
Midterm #1 Study Guide
Midterm #1 Study Guide

... What is the difference between mitosis and meiosis? Where do these processes occur? What are the results from each? Proteins associated with DNA in eukaryotes are called ______. Histone–DNA units are called _______. Chromatids that are attached at the centromere are called what kind of chromatids? ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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