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Unit 3 Practice Test
Unit 3 Practice Test

... d. with twice the number of chromosomes of a diploid cell. ______2. The members of a homologous pair of chromosomes a. are identical in size and appearance. b. contain identical genetic information. c. separate to opposite poles of the cell during mitosis. d. are found only in haploid cells. ______3 ...
DNA PPT - Lyndhurst School District
DNA PPT - Lyndhurst School District

... may result in harmful, beneficial, or neutral effects to the structure and function of the organism. [Clarification Statement: Emphasis is on conceptual understanding that changes in genetic material may result in making different proteins.] [Assessment Boundary: Assessment does not include specific ...
Analysis of DNA polymerase activity in vitro using non
Analysis of DNA polymerase activity in vitro using non

... ABSTRACT. Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used ...
Lab #1: Alu Lab, Part 1
Lab #1: Alu Lab, Part 1

... break the hydrogen bonds holding the two strands together. This allows each strand to serve as a template for generating copies of the DNA. In the second step, the temperature is reduced to allow the primers to anneal, or bind, at their complimentary sequence on the template. In the third step, the ...
Chapter 18 Practice Multiple Choice
Chapter 18 Practice Multiple Choice

... signal molecules produced by the maturing zygote ubiquitous enzymes such as DNA polymerase and DNA ligase paternally deposited proteins specific operons within the zygote genome ...
Welcome! 3/21/14
Welcome! 3/21/14

... does not always result in a visible change. n  Mutations may change the DNA but not the amino acid n  That mutations MAY result in a change in the PHENOTYPE of an organism, but not always. n  Think-Pair-Share n  WHY ...
BIO 208: GENETICS
BIO 208: GENETICS

Genetic engineering
Genetic engineering

... A cloning vector is a piece of DNA that can accept the target gene and replicate e.g. plasmid in bacteria. Restriction enzymes cut DNA at specific sites. Genetic ‘scissors’ that recognises a specific sequence of bases. DNA ligase is an enzyme that is used to get the foreign DNA to join with the DNA ...
Biology Final Review
Biology Final Review

... Name the 4 bases of DNA and how do they connect to each ...
Imprinting and Dosage Compensation-2015
Imprinting and Dosage Compensation-2015

... A long ncRNA Controls Imprinting at the Igf2r Locus ...
The biomolecules of terrestrial life
The biomolecules of terrestrial life

... Short chains of amino acids are called peptydes Long, unbranched peptyde chains are called polypeptides Proteins are formed by one or more chains of polypeptides Molecular masses of proteins vary between ~103 e ~106 atomic mass units They contribute to about half the mass of the cell ...
Unit 3 PowerPoint
Unit 3 PowerPoint

... process and the offspring were essentially a "dilution"of the different parental characteristics. ...
Point Mutation Detection
Point Mutation Detection

... is extracted and the DNA is visualized and/or prepared for subsequent analysis by a number of techniques including restriction fragment length polymorphism (RFLP) and Southern blotting, DNA amplification using the polymerase chain reaction (PCR), or DNA sequence analysis. RFLP and Southern Blot Anal ...
Lecture 1. - Government Degree College Pulwama
Lecture 1. - Government Degree College Pulwama

... sequences that would not otherwise be found in the genome. Recombinant DNA is the general name for a piece of DNA that has been created by the combination of at least two strands. Recombinant DNA molecules are sometimes called as Chimeric DNA, because they can be made of material from two different ...
Lectures 1 & 2 (2010.03.05 & 2010.03.06)
Lectures 1 & 2 (2010.03.05 & 2010.03.06)

... (A) The cloverleaf structure, a convention used to show the complementary base-pairing (red lines). The ANTICODON is the sequence of three nucleotides that base-pairs with a CODON in mRNA. The amino acid matching the codon/anticodon pair is attached at the 3′ end of the tRNA. tRNAs contain some unus ...
Detection of Transcription Factor Binding Sites
Detection of Transcription Factor Binding Sites

... which can be used by a cell to construct proteins  Each set of instructions within this sequence is called a gene ...
chapter 3
chapter 3

... segment of DNA that is to be amplified. The length of the primers usually 20 bases or more must be sufficient to overcome the statistical likelihood that their sequence would occur randomly in the overwhelmingly large number of non-target DNA sequences in the sample. PCR is carried out in a series o ...
Transcription Translation Powerpoint
Transcription Translation Powerpoint

... What do you think would happen if there was a mistake in transcription? ...
Recitation Section 16 Answer Key Recombination and Pedigrees
Recitation Section 16 Answer Key Recombination and Pedigrees

... 5. What is your prediction for mode of inheritance of this disease? Why? Because the phenotype is so rare, the disease is likely to be recessive. It is in fact recessive and very rare. 6. The fact that Garrod saw a number of cases was in fact due to high degree of first cousin marriages in the com ...
Disorders associated with mutations in the POLG gene
Disorders associated with mutations in the POLG gene

... Oxford Medical Genetics Labs ...
Supplementary information - Springer Static Content Server
Supplementary information - Springer Static Content Server

... activity of their rpoS gene 6–8. RpoS has an important role under stationary phase conditions, in particular because it controls synthesis of a protein that should have an important role in our experiment, the ribosome trigger factor (TIG), involved in the folding of proteins in statu nascendi 9. We ...
Mitochondria tutorial
Mitochondria tutorial

... default 'all restriction enzymes'. There are a lot of different enzymes, and some of them are pretty difficult (and expensive) to buy, so we will change the setting to Core set of enzymes. This way the program will only search enzymes that are common, usually inexpensive, and frequently found on the ...
Gene therapy and viral vector
Gene therapy and viral vector

Biology 2
Biology 2

... A change in the DNA sequence that affects the structure of the proteins. Almost all the nonsense amino acids, 70%, will be miscoded and usually new stops result in the codon. In cystic fibrosis, the condition can be traced back through the difference in a protein to one tiny change in a gene. In the ...
1 A. You have the following piece of genomic DNA with the two
1 A. You have the following piece of genomic DNA with the two

... 12. Name another alternative strategy that can be used to confirm this conclusion? 13. Describe the steps you would use to perform this second alternative strategy? 14. Taking into account the conclusions you have made up to this point, examine the provided sequence. What can you conclude about the ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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