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Mismatch repair
Mismatch repair

... 2. Deamination (脱胺作用) : C --> U, A --> Hypoxanthine (次黄嘌呤) ...
BB30055: Genes and genomes
BB30055: Genes and genomes

... signal transduction and immune function) However, only 3 cases where a combination of 3 domain types shared by human & yeast proteins. e.g carbomyl-phosphate synthase (involved in the first 3 steps of de novo pyrimidine biosynthesis) has 7 domain types, which occurs once in human and yeast but twice ...
The ATM repair pathway inhibits RNA polymerase I transcription in
The ATM repair pathway inhibits RNA polymerase I transcription in

... when and how much of a gene product will be produced. ...
14–16 Video transcript: Chickens and Campylobacter
14–16 Video transcript: Chickens and Campylobacter

119KB - NZQA
119KB - NZQA

... therefore show the trait. But neither does, so non-sneezing cannot be dominant. 13 is a sneezer so must have A. They could be AA or Aa as each parent can pass on either. The Punnett square shows that 25% are expected to be AA and 50% Aa. Without offspring we can’t prove ...
Disclaimer: Not ALL of the questions on the midterm will necessarily
Disclaimer: Not ALL of the questions on the midterm will necessarily

... bacterial gene has a base sequence 5’ ACAGGC. Draw and label a diagram of how this sequence would be copied during transcription. Which enzyme is responsible for transcription? And name the three dif rent molecules made by transcription. ...
DNA Repair - WordPress.com
DNA Repair - WordPress.com

... opposite to thymine dimers. But sometimes, Pol V does errors for unknown reasons, especially during stress. One possible reason for this is that the error prone polymerase may have developed by evolutionary processes. They create mutations at a time when the cell might need variability. In the secon ...
NCEA Level 1 Science (90948) 2016
NCEA Level 1 Science (90948) 2016

... therefore show the trait. But neither does, so non-sneezing cannot be dominant. 13 is a sneezer so must have A. They could be AA or Aa as each parent can pass on either. The Punnett square shows that 25% are expected to be AA and 50% Aa. Without offspring we can’t prove ...
Lecture 7 Mutation and its consequences CAMPBELL BIOLOGY
Lecture 7 Mutation and its consequences CAMPBELL BIOLOGY

... • DNA  Ancestry  and  Family  Origin  (FTDNA  affiliate  in  the  Middle  East)  (adop-on,  deep  ancestry,  full  mtDNA  sequencing,  genealogy)     • DNA  Canada  (genealogy,  paternity,  rela-onship)     • DNA  Diagnos-cs  Center  (adop-on, ...
No Slide Title
No Slide Title

... • Longer time to accumulate introns? • Genomes are more recombinogenic due to repeated sequences? • Selection for increased protein complexity – Gene number does not correlate with complexity – Ergo, it must come from somewhere ...
Complex Germline Architecture: Two Genes
Complex Germline Architecture: Two Genes

... sometimes called nanochromosomes (Doak et al. 2003) because of their size and because they typically contain just one gene each. These together comprise the gene-dense somatic genome. The process of deletion of up to 98% of the germline DNA removes internal eliminated segments (IES) that interrupt g ...
(HSV) PCR, CSF
(HSV) PCR, CSF

... •Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by HSV DNA is not likely. •This assay may detect viral sheddi ...
PDF
PDF

... ensuring the formation of an unobstructed lumen. But in many developmental contexts, tubes form from simple cords of cells, which presents a unique challenge for the formation of a continuous lumen. On p. 2985, Di Jiang and colleagues investigate how this challenge is overcome during tubulogenesis i ...
nucleic acids 3115
nucleic acids 3115

... 1. Describe and explain the basic structure of DNA and RNA 2. Describe and explain the function of DNA and RNA 4. Describe and explain the process of DNA replication 6. Describe and explain the basic structure of a nucleotide 7. Describe and explain the function(s) of a nucleotide 8. Be able to use ...
SCIENCE PROFICIENCY STUDY GUIDE – LIFE SCIENCE
SCIENCE PROFICIENCY STUDY GUIDE – LIFE SCIENCE

... 10. What term describes the ability of an organism to survive and reproduce in its environment? A. Evolution B. Fitness C. Artificial selection D. Natural selection 11. An individual in a population that is considered the most fit would A. live the longest. B. consume the most food. C. produce the m ...
Molecular Biology DNA Expression
Molecular Biology DNA Expression

Name: Date: Period: Part I. The Lac Operon. Follow this link: http:
Name: Date: Period: Part I. The Lac Operon. Follow this link: http:

... Drag the lac promoter to the stretch of DNA. Do NOT drag the lacZ gene to the DNA. What happens? Why is this? ...
Megan Silas - University of Illinois at Chicago
Megan Silas - University of Illinois at Chicago

... CAIR will disappear as it is converted into NCAIR by PurE Measure change in absorbance due to disappearance of CAIR Compare rate of reaction catalyzed by WT PurE versus H70N and truncated PurE Meyer, E., N.J. Leonard, B. Bhat, J. Stubbe, and J.M. Smith. "Purification and characterization of the purE ...
DNATeachPrep
DNATeachPrep

... Virtual Lab (available at http://learn.genetics.utah.edu/content/labs/extraction/), either of which can be used together with our analysis and discussion activity "DNA Structure, Function and Replication" (available at http://serendip.brynmawr.edu/exchange/bioactivities/DNA). Learning Goals In accor ...
Chapter 22. Nucleic Acids
Chapter 22. Nucleic Acids

... the nuclei of white blood cells. In the 1920's nucleic acids were found to be major components of chromosomes, small gene-carrying bodies in the nuclei of complex cells. Elemental analysis of nucleic acids showed the presence of phosphorus, in addition to the usual C, H, N & O. We now know that nucl ...
Introduction to Molecular Pathology
Introduction to Molecular Pathology

... mRNA degradation RNA interference or silencing  miRNA and siRNA ...
Modular proteins I
Modular proteins I

switchSENSE® compatibility sheet
switchSENSE® compatibility sheet

... DNA duplex integrity ([Na+] or [K+] > 20 mM). Divalent cations can also be added, e.g. [Mg2+] = 5 mM, to further increase duplex stability. When using single stranded DNA or PNA/DNA hybrids, very low salinity (1 mM and lower) can be used. The addition of a surfactant (e.g. Tween) is recommended. For ...
Metagenomics - University of Maryland, College Park
Metagenomics - University of Maryland, College Park

... genetic sequences of unidentified, unculturable bacteria to that of known, culturable ones, in order to come to a conclusion about the evolutionary origins of the unculturable bacteria. • The main source of genetic material used to study evolutionary relationships is the 16S rRNA subunit. The 16S rR ...
Simulation of Gene Splicing (Genetic Engineering
Simulation of Gene Splicing (Genetic Engineering

... What sticky ends have you made on the human DNA containing the growth hormone gene? What sticky ends have you made on the bacterial DNA (plasmid)? Compare the two. What do you observe? Once the recombinant DNA you just constructed was in existence, the next step would be to insert it into a new bact ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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