Supplementary Information (doc 417K)
... Nuclear extracts were prepared as previously described (Spruijt et al., 2013). Briefly, cells were washed with PBS and incubated in hypotonic buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM MgCl2) for 15 minutes and homogenized using a type B (tight) pestle in the presence of 0.15% NP-40 (Roche) and ...
... Nuclear extracts were prepared as previously described (Spruijt et al., 2013). Briefly, cells were washed with PBS and incubated in hypotonic buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM MgCl2) for 15 minutes and homogenized using a type B (tight) pestle in the presence of 0.15% NP-40 (Roche) and ...
The Dna code - Winston Knoll Collegiate
... DNA stores information to build proteins in sequences of nucleotides - DNA nucleotides contain one of 4 nitrogen bases A T C G - there are 20 different amino acids used to build protein ...
... DNA stores information to build proteins in sequences of nucleotides - DNA nucleotides contain one of 4 nitrogen bases A T C G - there are 20 different amino acids used to build protein ...
Bio 392: Study Guide for Final
... o The steps of the cell cycle- what happens during each phase o The steps of mitosis- what is happening with the chromosomes in each o Process of cytokinesis o Differences between cell division in plant and animal cells ...
... o The steps of the cell cycle- what happens during each phase o The steps of mitosis- what is happening with the chromosomes in each o Process of cytokinesis o Differences between cell division in plant and animal cells ...
lecture - Haloarchaea
... Hill, C. W., and J. A. Gray. 1988. Effects of chromosomal inversion on cell fitness in Escherichia coli K-12. Genetics 119:771–778. Eisen, J. A., J. F. Heidelberg, O. White, and S. L. Salszberg. 2000. Evidence for symmetric chromosomal inversions around the replication origin in bacteria. Genome Bio ...
... Hill, C. W., and J. A. Gray. 1988. Effects of chromosomal inversion on cell fitness in Escherichia coli K-12. Genetics 119:771–778. Eisen, J. A., J. F. Heidelberg, O. White, and S. L. Salszberg. 2000. Evidence for symmetric chromosomal inversions around the replication origin in bacteria. Genome Bio ...
Gene Section NEIL1 (nei endonuclease VIII-like 1 (E. coli))
... deoxyribo-5'-phosphate (dRP) and excised by a dRP lyase (dRPase) activity of DNA polymerase beta. Since NEIL1 also has dRPase activity, NEIL1 has a role as a backup dRPase in mammalian cells. (5) NEIL1 has a repair activity for oxidized bases in single-strand DNA and bubble DNA, suggesting a possibi ...
... deoxyribo-5'-phosphate (dRP) and excised by a dRP lyase (dRPase) activity of DNA polymerase beta. Since NEIL1 also has dRPase activity, NEIL1 has a role as a backup dRPase in mammalian cells. (5) NEIL1 has a repair activity for oxidized bases in single-strand DNA and bubble DNA, suggesting a possibi ...
PartTwoAnswers.doc
... chromosome this would be the last Okazaki fragment synthesized by the fork moving in the opposite direction. Action by a 5' to 3' exonuclease and polymerase (e.g. DNA Pol I) to replace the RNA primer at the 5' end of the Okazaki fragment, followed by ligase, would join the products from the two repl ...
... chromosome this would be the last Okazaki fragment synthesized by the fork moving in the opposite direction. Action by a 5' to 3' exonuclease and polymerase (e.g. DNA Pol I) to replace the RNA primer at the 5' end of the Okazaki fragment, followed by ligase, would join the products from the two repl ...
baby joe
... Part III—Immunoglobulin and T Cell Receptor Genetics Baby Joe’s doctors diagnosed his condition as severe combined immunodeficiency (scis). This disease is life-threatening; without treatment, it is usually fatal in childhood. scis can be caused by several genetic mutations. Many of these mutations ...
... Part III—Immunoglobulin and T Cell Receptor Genetics Baby Joe’s doctors diagnosed his condition as severe combined immunodeficiency (scis). This disease is life-threatening; without treatment, it is usually fatal in childhood. scis can be caused by several genetic mutations. Many of these mutations ...
A selfish origin for recombination
... (Burt, 2000) that recombination allows different combinations of alleles to be present in different individuals, that is to produce variability on which natural selection can act. Most theories (discussed in Kondrashov, 1993; Barton and Charlesworth, 1998) belong to one of two categories: either rec ...
... (Burt, 2000) that recombination allows different combinations of alleles to be present in different individuals, that is to produce variability on which natural selection can act. Most theories (discussed in Kondrashov, 1993; Barton and Charlesworth, 1998) belong to one of two categories: either rec ...
AP Biology Exam Review T2
... CAP (catabolic Activator Protein)/cAMP, glucose relationship Differential gene expression Role of histone modification/DNA methylation on eukaryotic gene expression Explain how enhancers and activator interact with transcription factors to affect gene expression Describe how proteins can be activate ...
... CAP (catabolic Activator Protein)/cAMP, glucose relationship Differential gene expression Role of histone modification/DNA methylation on eukaryotic gene expression Explain how enhancers and activator interact with transcription factors to affect gene expression Describe how proteins can be activate ...
MCB 135K: Discussion
... Fitness = reproductive success Individuals with the best reproductive success have more offspring. And so on, and so on, until the adaptation (gene) that led to greater reproductive success is present throughout the species. ...
... Fitness = reproductive success Individuals with the best reproductive success have more offspring. And so on, and so on, until the adaptation (gene) that led to greater reproductive success is present throughout the species. ...
LECTURE #20: Bacterial Transformation and Gel
... http://highered.mcgrawhill.com/sites/0072556781/s tudent_view0/chapter13/ani mation_quiz_1.html ...
... http://highered.mcgrawhill.com/sites/0072556781/s tudent_view0/chapter13/ani mation_quiz_1.html ...
NAME HONORS BIO CLASSIFICATION TEST VERSION A
... 14. The Greek philosopher and teacher who first classified organisms into two groups -- plants and animals was ________________________ A. Carolus Linnaeus B. Aristotle C. Gregor Mendel D. Nicholas Flamel 15.The molecule found in the cell walls of Fungi which makes them different from plants is ____ ...
... 14. The Greek philosopher and teacher who first classified organisms into two groups -- plants and animals was ________________________ A. Carolus Linnaeus B. Aristotle C. Gregor Mendel D. Nicholas Flamel 15.The molecule found in the cell walls of Fungi which makes them different from plants is ____ ...
Document
... Plant mitochondria “edit” their RNA transcripts. This was first noticed when comparing cDNA sequences with genomic DNA sequences. The most common change is to replace C with U, although in some instances other changes can occur. Matrix enzymes are thought to be responsible for this, but the reason f ...
... Plant mitochondria “edit” their RNA transcripts. This was first noticed when comparing cDNA sequences with genomic DNA sequences. The most common change is to replace C with U, although in some instances other changes can occur. Matrix enzymes are thought to be responsible for this, but the reason f ...
Construction and stable transformation of Tetrahymena
... main goals of Tetrahymena laboratories will be characterizing the unknown biological function of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published l ...
... main goals of Tetrahymena laboratories will be characterizing the unknown biological function of these genes in lifecycle by using functional complementation analysis based on phenotype with transfering genomic DNA library into mutant Tetrahymena cells using AC based vectors. There is no published l ...
Epigenetics
... Loss of H4K16 acetylation and H4K20 tri-methylation When tumor suppressor genes are down-regulated by hypermethylation, oncogenes may be stimulated by acetylation or hypomethylation Example: hypermethylation of H3K79 promotes leukemogenesis Tumor-specific epigenetic abnormalities can stem from a ...
... Loss of H4K16 acetylation and H4K20 tri-methylation When tumor suppressor genes are down-regulated by hypermethylation, oncogenes may be stimulated by acetylation or hypomethylation Example: hypermethylation of H3K79 promotes leukemogenesis Tumor-specific epigenetic abnormalities can stem from a ...
Restriction Digest of pAMP and pKAN
... It is important at this stage of our experimental procedure that we need to onfirm that Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular siz ...
... It is important at this stage of our experimental procedure that we need to onfirm that Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular siz ...
Evolution review!
... A mouse skin cell contains 40 chromosomes. Based on this information, how many chromosomes would a mouse SPERM cell contain? ...
... A mouse skin cell contains 40 chromosomes. Based on this information, how many chromosomes would a mouse SPERM cell contain? ...
Genetic recombination and mutations - formatted
... The answer to the question as to “How does the genetic variation arise?” lies in understanding the phenomena of mutation. Mutation can be defined as the occurrence of any change in the sequence of nucleic acid or any change in the chromosomal structure. Mutations can also be defined as heritable cha ...
... The answer to the question as to “How does the genetic variation arise?” lies in understanding the phenomena of mutation. Mutation can be defined as the occurrence of any change in the sequence of nucleic acid or any change in the chromosomal structure. Mutations can also be defined as heritable cha ...
lab9
... Concentrations of solutions - Higher concentrations of agarose facilitate separation of small DNAs, while low agarose concentrations allow resolution of larger DNAs. – Low conc. = larger pores better resolution of larger DNA fragments – High conc. = smaller pores better resolution of smaller DNA f ...
... Concentrations of solutions - Higher concentrations of agarose facilitate separation of small DNAs, while low agarose concentrations allow resolution of larger DNAs. – Low conc. = larger pores better resolution of larger DNA fragments – High conc. = smaller pores better resolution of smaller DNA f ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.