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No Slide Title
No Slide Title

... 1) Engineers make the design and tell the workers how to make the cars; 2) Workers follow the directions to build the cars; 3) Suppliers bring parts to the assembly line so they can be installed in the car ...
Chapter 27 Notes ppt
Chapter 27 Notes ppt

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ISTANBUL MEDIPOL UNIVERSITY Course Learning Outcomes of
ISTANBUL MEDIPOL UNIVERSITY Course Learning Outcomes of

... 2.An Introduction to Taxonomy: The Bacteria 2.1.Discuss how and why microorganisms are named. 2.2.Describe the five kingdom classification system, and place organisms in the correct kingdom based on their cell type (prokaryotic or eukaryotic), number of cells (unicellular or multicelluar), and metho ...
Polyploid Genomics
Polyploid Genomics

... ◦ Gradual conversion from polyploidy to diploidy through genetic changes that differentiate duplicated loci ...
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Eukaryotic Gene Regulation
Eukaryotic Gene Regulation

PowerPoint Presentation - Chapter 17 From Gene to Protein.
PowerPoint Presentation - Chapter 17 From Gene to Protein.

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File - El Paso High School
File - El Paso High School

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Chapter 12 Notes
Chapter 12 Notes

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Some - Laker Science
Some - Laker Science

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Genetics and Genomics in Medicine Chapter 7 Questions Multiple
Genetics and Genomics in Medicine Chapter 7 Questions Multiple

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AP Protein Sythesis

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Specialized Transduction
Specialized Transduction

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CRISPR| Cas Gene Editing - Federation of American Societies for

... Research continues at a rapid pace, refining the technique with new nucleases and new ways to deliver genes to cells. For example, a strain of mice engineered to express the Cas9 protein has been developed so that researchers can edit genes by simply introducing the guideRNA. Although permanent elim ...
HS-LS1-1 Taco Protein Synthesis Activity.docx
HS-LS1-1 Taco Protein Synthesis Activity.docx

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Biology QUIZ: 13-2 and 13-3 Multiple Choice Identify the choice that

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Biochemistry - Stryer - Science and Technology

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hybrid DNA molecules
hybrid DNA molecules

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... insert is used as poly (A) addition signal. If it is, the 5' noncoding sequence must be extremely long (about 2.5-kbp), since the ear-3 mRNA are 4.6-kb and 4.8-kb long. The free energy of a possible secondary structure at the 5' untranslated region (32) was calculated to be -207.4 kcal. Thus, this r ...
RIBONUCLEIC ACID (RNA)
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How Do Heritable Changes in Genes Occur?
How Do Heritable Changes in Genes Occur?

... transcription and replication of the genes in which they are present, these lesions are fatal if they go uncorrected. Not surprisingly, therefore, organisms (including ourselves) have a number of different DNA repair systems for eliminating such damage from DNA. But it is the very act of attempting ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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