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CHAPTER 17 - HCC Learning Web
CHAPTER 17 - HCC Learning Web

... Elongation of the Polypeptide Chain • During the elongation stage, amino acids are added one by one to the preceding amino acid at the C-terminus of the growing chain • Each addition involves proteins called elongation factors and occurs in three steps: codon recognition, peptide bond formation, an ...
f 1 - GForge
f 1 - GForge

... using discrete values (binary, integer, or any other system with a discrete set of ...
NAME :Abubakar Aisha MATRIC NO:14/sci05/001 DEPT
NAME :Abubakar Aisha MATRIC NO:14/sci05/001 DEPT

... A mutation is a permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extra chromosomal DNA or other genetic elements. Mutations result from damage to DNA which is not repaired, errors in the process of replication, or from the insertion or deletion of segments of D ...
Presentazione standard di PowerPoint
Presentazione standard di PowerPoint

... Biomolecules are the main components of our body and of the body of all living organisms. They are complex molecules, very complex in some cases, but they are part of our everyday life although most of us are not aware of this. In fact, together with vitamins and minerals, they are the basis of our ...
Prokaryotic Gene Regulation
Prokaryotic Gene Regulation

... • Effector molecules bind to regulatory proteins and not to DNA directly (either increase or inhibit transcription) - Inducers increase transcription by either: ¾Bind activators and cause them to bind to DNA ¾Bind repressors and prevent them from binding to DNA - Inhibitors of transcription (2 types ...
Sample pages 2 PDF
Sample pages 2 PDF

... pore from a donor cell to a recipient cell. A single strand of DNA is transferred and is copied in the recipient, while the other single strand is copied in the donor. This process is commonly mediated by plasmid systems that can either mediate their own transmission (“self-transmissible”) or transf ...
Introduction Kit components
Introduction Kit components

... filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. It applies the principle of a mini-column spin technology and is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after res ...
Methylation and demethylation of DNA and histones in
Methylation and demethylation of DNA and histones in

... modifications regulate chromatin dynamics by preparing specific loci for activation or repression ultimately governing the global chromatin architecture with regard to gene expression during the cell cycle and development. Methylation is the most complicated epigenetic modification. Unlike other change ...
Exam - National Biology Competition
Exam - National Biology Competition

... c. Myelin decreases intracellular resistance, which causes the action potentials to decay at a slower rate. d. Myelin decreases intracellular resistance, which causes the action potentials to decay at a faster rate. e. Myelin increases the membrane resistance and decreases the intracellular resistan ...
Readings Problems Background Week 8
Readings Problems Background Week 8

... Thus, the above observations meant that the “messenger” (as it was eventually named by Jacob, who initially called it “X”) had to be unstable and that it was not ribosomal RNA. The only other cellular RNA known at the time was "soluble RNA" (transfer RNA), too small to code for most proteins. Then w ...
9/17/08 Transcript I
9/17/08 Transcript I

...  The chain elongation, involves the core polymerase with no sigma factor involved.  Polymerase is very accurate, only about 1 error in 10,000 bases. That may seem high, but its not because many transcripts are made from each individual gene, so these errors can occur in many different places and e ...
File - Mrs. LeCompte
File - Mrs. LeCompte

Thursday, 1. October 2015
Thursday, 1. October 2015

... toriographical!Actors!Get!a!Life!of!Their!Own]! ...
ppt - Duke Computer Science
ppt - Duke Computer Science

... Columbia University ...
ch_07_clicker_questions
ch_07_clicker_questions

... Which statement regarding prokaryotic chromosomes is incorrect? a) There are two copies of the chromosome so that cells are diploid. b) There is a single copy of the chromosome so that cells are haploid. c) Prokaryotic chromosomes are located in the nucleoid. d) Prokaryotic chromosomes are now know ...
RNA and DNA aptamers. Ribozymes and DNAzymes Daniel
RNA and DNA aptamers. Ribozymes and DNAzymes Daniel

... Columbia University www.columbia.edu/cu/biology/courses/w3034/Larry/class26_11plus.ppt ...
Minimum Entropy Approach to Word Segmentation Problems by Bin
Minimum Entropy Approach to Word Segmentation Problems by Bin

... DNA is a text written with 4 distinct “letters”. However, different from an ordinary text the DNA strand is a single long sentence devoid of delimiters such as space, comma, period, etc. In order to be able to “read” the information of the DNA, the first step is to be able to identify the “words” in ...
Chapter 4 - Chemical Engineering - Michigan Technological University
Chapter 4 - Chemical Engineering - Michigan Technological University

... Repression of Transcription (m-RNA) an end product of enzyme activity or of the metabolic pathway (co-repressor) blocks m-RNA synthesis ...
Nitrogen is the stuff of life. Ironically the prefix azo
Nitrogen is the stuff of life. Ironically the prefix azo

... DNA and proteins, the genetic code and building blocks of all living things, contain nitrogen at pivotal points in their structure. ...
Preparation and analysis of environmental DNA: optimisation of
Preparation and analysis of environmental DNA: optimisation of

... to which interfering organic and inorganic impurities are present pose a problem for molecular analyses. These issues are important as subsequent analysis by polymerase chain reaction (PCR) may be problematic with sheared DNA or may not occur at all in the presence of certain impurities. Any loss of ...
A Recipe for Traits - Learn Genetics (Utah)
A Recipe for Traits - Learn Genetics (Utah)

... base is referred to by the first letter of its name: Adenine (A), Cytosine (C), Guanine (G) and Thymine (T). The sequence of these chemical bases encodes a detailed set of instructions for building an organism’s traits. (The human genome contains approximately 3 billion pairs or bases!) • Participa ...
GMO answerkey
GMO answerkey

... Boyer’s work in the early 1970s focused on the activity of a class of enzymes called restriction endonucleases that targeted particular sequences of nucleotides and cut DNA molecules at these sites. His belief was that such molecules would have “sticky ends” that could be joined to other DNA molecu ...
Document
Document

... The virus is allowed to reproduce in culture, where it copiously makes new copies of itself The phage virus displays these proteins on the surface of the virions, Selection is done in vitro by simply passing the viral stew over a stationary phase containing the target substrate. Those that can bind ...
Ribosome - Mrs. J. Malito
Ribosome - Mrs. J. Malito

... New mRNA breaks away from the leading DNA strand and the two original DNA strands rejoin and wind back up. ...
1305077113_457396
1305077113_457396

... Trace ancestry through the male line ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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