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MT03
MT03

... INVOLVES NON-SEGREGATION OF HOMOLOGOUS CENTROMERES. ADJACENT-1 SEGREGATION HAS HOMOLOGOUS CENTROMERES SEGREGATING NORMALLY. 11. Assume that there are five sequential steps in the formation of wild-type fur in mice (agouti), and that each step in this pathway is mediated by a different gene product. ...
DNA and Genetics
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... events in the stages of both types of cell division. Comparisons and similarities between the two types of cell division should be made, as well as highlighting the differences. This topic presents a good opportunity for the learners to research and present this information to the rest of the class. ...
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... the fly or worm because of mRNA transcript "alternative splicing" and chemical modifications to the proteins. This process can yield different protein products from the same gene. • Humans share most of the same protein families with worms, flies, and plants; but the number of gene family members ha ...
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DNA, Inheritance, and Genetic Variation

... This time, however, because there are • Compare and contrast mitosis two meiotic divisions, students will and meiosis in terms of number observe that the gametes formed by of cells produced, diploid and meiosis have only half the number of haploid numbers, and genetic chromosomes of the parent cell ...
Restriction mapping
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BACK TO GAME - demascalchemistry

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HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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