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Structure and Physiological significance of lipid
Structure and Physiological significance of lipid

... DNA (rDNA) is a form of artificial DNA that is created by combining two or more sequences that would not normally occur together through the process of gene splicing. Recombinant DNA technology is a technology which allows DNA to be produced via artificial means. The procedure has been used to chan ...
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... Recombinant DNA (rDNA) is a form of artificial DNA that is created by combining two or more sequences that would not normally occur together through the process of gene splicing. Recombinant DNA technology is a technology which allows DNA to be produced via artificial means. The procedure has been u ...
Genetics and Genomics in Medicine Chapter 7 Questions
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... inactivate a splice acceptor site, and might lead to exon skipping. If so it will produce a frameshift because the exon has 56 nucleotides, a number that is not a multiple of three. 2) Deletion of a single amino acid. In this case a cysteine is deleted, which may cause major protein structure diffic ...
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... B) It produces a complementary copy of a strand of DNA. C) It constructs RNA chains using a template from DNA genes. D) It retrieves amino acids from the cytoplasm for protein construction. 71. Some organelles have their own DNA that is distinct from the cell’s nuclear DNA. This is true of which org ...
CHAPTER 12 - powerpoint
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... • Certain viruses use RNA rather than DNA as their information molecule during transmission. • These viruses transcribe from RNA to RNA; they make a complementary RNA strand and then use this “opposite” strand to make multiple copies of the viral genome by transcription. • HIV and certain tumor viru ...
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End-of-Course

... 16. Enzymes are proteins that help increase the rate of chemical reactions inside cells. These proteins are composed of many simpler molecules called amino acids. Which of the following suggests that the shape of an enzyme determines the enzyme’s function? F Enzymes are specific to a substrate. G En ...
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... are what characterize the differences between alleles. There are 3 known snips in the gene TAS2R38. The most common one, located at the 785 nucleotide position of the DNA template strand, is associated with a loss of function in the protein product. This particular snip is a transition mutation from ...
Transcription in Bacteria
Transcription in Bacteria

... During initial transcription, RNA polymerase produces and releases short RNA transcripts of less then ten ribonucleotides (abortive synthesis) before escaping the promoter (promotor clearance). It is not clear why RNA polymerase must undergo this period of abortive initiation before achieving escape ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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