• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Meiosis
Meiosis

... Meiosis I (reductional division) Homologous chromosomes are separated into different cells: each daughter cell contains one duplicated copy of each type of chromosome (the number of chromosomes is cut in half) Meiosis II (equational division) Sister chromatids are separated into different cells (lik ...
The genomic landscape of meiotic crossovers and gene
The genomic landscape of meiotic crossovers and gene

The Chromosomal Basis of Inheritance
The Chromosomal Basis of Inheritance

... Parents: YyRr (yellow, round) and yyrr (green, wrinkled) • Some offspring are (yellow, wrinkled) and (green, round)… • This is recombination!  ...
Heterocyclic compounds with biological meaning NEW
Heterocyclic compounds with biological meaning NEW

... DNA structure • DNA consists of two helical chains wound around the same axis in a right-handed fashion aligned in an antiparallel fashion. • There are 10.5 base pairs, or 36 Å, per turn of the helix. • Alternating deoxyribose and phosphate groups on the backbone form the outside of the helix. • Th ...
Bacterial Gene Swapping in Nature
Bacterial Gene Swapping in Nature

... form between donor and recipient cells, and plasmids pass through the bridges from the donors to the recipients. Some pili can promote aggregation of bacterial cells in liquid and on solid surfaces; others can stimulate aggregation efficiently only on solid surfaces. Such differences imply that rese ...
DNA BASE PAIR “Friendship Bracelets” Background: DNA is the
DNA BASE PAIR “Friendship Bracelets” Background: DNA is the

RNA Structure, Function, and Synthesis RNA - Rose
RNA Structure, Function, and Synthesis RNA - Rose

... the lac repressor binds to the promoter, and prevents transcription. When lactose is present, it binds to the repressor; the repressor then dissociates from the DNA, allowing transcription of the genes. However, if glucose levels are also high, the resulting low levels of cAMP do not allow cAMP rece ...
Epigenetics Article
Epigenetics Article

... instruction book for the human body. But genes themselves need instructions for what to do, and where and when to do it. A human liver cell contains the same DNA as a brain cell, yet somehow it knows to code only those proteins needed for the functioning of the liver. Those instructions are found no ...
Epigenetics and Inheritance
Epigenetics and Inheritance

... expression”. (1) In Greek, the prefix epi means upon, above, in addition to, or near, so epigenetics means a way of changing the expression of genes without changing the DNA sequence. (2) The word epigenetics is derived from the word epigenesis which “can be traced back to Aristotle, who proposed th ...
$doc.title

... using   a   simple   StringBuilder   to   represent   the   DNA/enzymes,   you’ll   use   a   linked-­‐list   implementation   that   makes   the   complexity   of   the   splicing   independent   of   the   size   of   the   spliced-­‐in   stran ...
Correspondence
Correspondence

... schistosomiasis,2 myotonic dystrophy,3 elephantiasis4 and AntleyBixler syndrome have also been suggested.5 More recently, we proposed homocystinuria as a possible cause.6 Since Kallman’s syndrome is a hereditary disease, it is important to see how this diagnosis fits into Akhenaten’s family tree. Th ...
neutral theory, inbreeding - Cal State LA
neutral theory, inbreeding - Cal State LA

... zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae zuleicae ...
IBC Reviewer Form - Benaroya Research Institute
IBC Reviewer Form - Benaroya Research Institute

... recombinant or synthetic nucleic acid molecules, or nucleic acids derived therefrom, into the germ-line (transgenic animals) and experiments involving viable recombinant or synthetic nucleic acid molecule-modified microorganisms tested on whole animals. For the latter, other than viruses which are o ...
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education

... • The s-factor changes its relationship to the core polymerase during elongation • It may not dissociate from the core but actually shift position and become more loosely bound to core • To answer this question Fluorescence Resonance Energy Transfer (FRET) was used as it relies on two fluorescent mo ...
Promoter identification
Promoter identification

... transcription factors to alternative control elements, permitting complex control of gene expression. • RNA polymerase I synthesizes rRNA. • RNA polymerase II synthesizes mRNA. • RNA polymerase III synthesizes small RNAs and tRNA. ...
DNase I (AMPD1) - Technical Bulletin - Sigma
DNase I (AMPD1) - Technical Bulletin - Sigma

... DNase I has been purified to remove RNase activity, and is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RTPCR (Reverse Transcriptase – Polymerase Chain Reaction). No current RNA isolation procedure removes 100% of the DNA. Because PCR can detect even a ...
Gene expression profiling during conjugation of the Tetrahymena
Gene expression profiling during conjugation of the Tetrahymena

... After running RT-PCR of the gene and analyzing the results on an agarose gel, different results were obtained. This experiment provided data that suggested the cyclin portion of the gene is only expressed between hours 8 and 14 of conjugation. At C-8, eight hours after mixing, macronuclear developm ...
Construction of a set of convenient saccharomyces cerevisiae
Construction of a set of convenient saccharomyces cerevisiae

Sex and Deleterious Mutations
Sex and Deleterious Mutations

... during N generations in such a way to reach an approximate mutation–selection equilibrium. At this time, we randomly choose an individual at which a modifier recombination allele becomes activated. At the recombination stage, we randomly arrange N/2 pairs of individuals. For each pair, if only one c ...
Mutation - SD43 Teacher Sites
Mutation - SD43 Teacher Sites

... A gene mutation results when the specific order of the A, G, C, and T bases that make up a particular gene changes. A mutation can occur any time in the life of a cell. Types of gene mutations include: • deletion (one base is missing) • addition (an extra base is added) • substitution (one base is s ...
Introducing: TGGE
Introducing: TGGE

... The DNA fragments are separated by their melting behavior. They can be distinguished as soon as the fragments begin to melt, i.e. they form a fork like structure. During electrophoresis the fragments should not separate into single strands. This is an irreversible transition resulting in diffuse ban ...
Assigned Study Questions Due on Monday, April 9, 2007
Assigned Study Questions Due on Monday, April 9, 2007

... 20) If the recombination frequency for Y and Z was found to be 50%, this would mean that A) genes X and Y are on the same chromosome. B) genes X and Y are on different chromosomes. C) genes Y and Z are on different chromosomes. D) both A and C. E) both B and C Answer: E 21) Which of the following is ...
Ghost in Your Genes
Ghost in Your Genes

... “Checking Up on Genes” student handout. If student DNA microarray results differ, ask students why that might be. (Some reasons include that the substances in the prepared microarray were not distributed evenly or that students may have added different amounts of the substance representing the cDNA. ...
Untitled
Untitled

... The function of these consensus sequences in bacterial promoters has been studied by inducing mutations at various positions within the consensus sequences and observing the effect of the changes on transcription. The results of these studies reveal that most base substitutions within the −10 and −3 ...
Conserved amino acid sequences confer nuclear localization
Conserved amino acid sequences confer nuclear localization

... Prophet of Pit-1 (PROP1) is a homeodomain transcription factor essential for development of the mammalian anterior pituitary gland. Studies of human patients and animal models with mutations in their Prop1 genes have established that PROP1 is required for the correct development or sustained functio ...
< 1 ... 130 131 132 133 134 135 136 137 138 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report