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point mutations - Plant Developmental Biology
point mutations - Plant Developmental Biology

... male bees, wasps, and ants are examples of monoploids monoploids are sterile (no meiosis possible and propagation via mitotic gametes) ...
Inferring Speciation Processes from Patterns of Natural Variation in
Inferring Speciation Processes from Patterns of Natural Variation in

... looking at dN /dS ratios between strains adapted to different light levels, the authors detected 78–90 genes that appeared to be under positive selection. However, 68 genes were found to be under positive selection when ...
Biology Keystone Review Packet
Biology Keystone Review Packet

... Nitogenous bases can be cytosine (C), thymine (T), uracil (U), adenine (A), or guanine (G) ...
BioTeke Corporation
BioTeke Corporation

... 1.Please add 60ml ethanol to 15ml Buffer WB before first time, vortex adequately, and then check it to avoid multi-adding! 2.Buffer CB or IR may precipitate under low temperature, incubate them at 37℃ water-bath for a moment until clear, then cool down to RT for use. 3.Please cap all reagents bottle ...
"Dot and Slot Blotting of DNA". In: Current Protocols in Molecular
"Dot and Slot Blotting of DNA". In: Current Protocols in Molecular

... dot blots prepared from DNA of various mammals. An intergenic or intronic region, which is less likely to have homologs in the other species, will probably not show widespread hybridization. This is the so-called “zoo blot” approach; blots containing DNA from a variety of related species are availab ...
Chapter 3
Chapter 3

... being produced. The structure of these chromosomes will be described in more detail in the discussion of cell division that follows. Chromatin consists of DNA and histone proteins. This association of DNA and protein helps with the complex jobs of packing DNA into chromosomes and regulating DNA acti ...
Presentation
Presentation

... • An SVM can discriminate between MNase protected and MNase accessible sequences with high accuracy. • The model learns to recognize complementary phenomena, depending upon the degree of MNase digestion. • The model recapitulates known features of ...
Solution to Practice Exam 2
Solution to Practice Exam 2

... The following pedigree represents the inheritance of PKA. Note: All affected individuals are shaded. Also listed are the alleles of SNP 1 and 2 for some individuals. People marrying into the family only have the wild-type copy of the PKA gene. Note: The two letters identify the alleles of the SNP th ...
Manual - The University of Iowa
Manual - The University of Iowa

... specified on the order, this is the backbone that will be used. ** This backbones express the transactivator for the 2 nd generation Tet-On system. ***The ∆1.6Kb and ∆3.1Kb have additional deletions in the E3 region, but no genes inserted into the region. These backbones are useful when attempting t ...
Nucleus All cells of the plant kingdom have a nucleus
Nucleus All cells of the plant kingdom have a nucleus

... proteins and nucleic acids found within the nucleus of cells is called the n________. A small rounded body within a nucleus that contains RNA and proteins and is involved in the production of ribosomes is known as the n______. The substance of a cell nucleus, consisting of DNA, RNA, and various prot ...
MOLLECULAR BIOLOGY COURSE
MOLLECULAR BIOLOGY COURSE

... How does a gene make a protein? Most genes contain the information needed to make functional molecules called proteins. (A few genes produce other molecules that help the cell assemble proteins.) The journey from gene to protein is complex and tightly controlled within each cell. It consists of two ...
Direct and indirect consequences of meiotic recombination
Direct and indirect consequences of meiotic recombination

... mismatches formed during homologous recombination. The second, which we term ‘hotspot drive’, results from the biased transmission of non-recombinogenic alleles over recombinogenic ones in hotspots of recombination [19]. The third, which we term ‘indel drive’, refers to the biased transmission of ei ...
DNA binding
DNA binding

... bacterial DNA, most of the viral genes remain unexpressed, allowing the viral genome to be carried along as the bacteria replicate. There are two key proteins and a set of regulatory sequences in the viral genome. They act as switches responsible for determining which of pathways to follow. ...
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... genes, or messenger RNAs, which are the RNAs that get translated into proteins. Also, most snRNA (splicing) and microRNAs (RNAi). This is the most studied type, and due to the high level of control required over transcription a range of transcription factors are required for its binding to promoters ...
The DpnI/DpnII pneumococcal system, defense against foreign
The DpnI/DpnII pneumococcal system, defense against foreign

... been proposed as the bacterial equivalent of eukaryotic sexual reproduction, promoting genetic diversity.1 Transformation involves internalization of foreign DNA in the form of single strands (ss), generated from a double-stranded (ds) substrate, which are recombined into the host genome by homology ...
ZGeneBio Urine Circulating Nucleic Acid Extraction Kit
ZGeneBio Urine Circulating Nucleic Acid Extraction Kit

Comprehension Questions
Comprehension Questions

... recessive mutation. If the heterozygote with the mutation on one chromosome and the deletion on the homologous chromosome has a mutant phenotype, then the mutation must be located on the same physical portion of the chromosome that is deleted. If, on the other hand, the heterozygote has a wild-type ...
Errors in the Code
Errors in the Code

... opposite direction. Any resulting protein would be seriously altered and probably nonfunctional. This type of mutation is called an inversion. Slide 11 That broken piece of chromosome may meet another fate. When a segment of chromosome breaks off one chromosome and is reinserted into a different chr ...
Using E. coli as a model to study mutation rates
Using E. coli as a model to study mutation rates

... history of an egg is 24. In males this depends on the number of stem-cell divisions, which is greater in older males. ...
corneagene_cm
corneagene_cm

... of type I lattice (Haab-Dimmer) dystrophy, which is one of the 5q31 related corneal dystrophies in 1995. The purpose of their former studies was the identification of the amyloid precursor in LCDI and its biochemical characterization. They also carried out immunohistochemical investigations in scarr ...
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... /genetic information/genes/DNA/ chromosomes… as the original cell OR To maintain the… number of chromosomes/chromosome complement… in new/daughter cells ...
MS-SCI-LS-Unit 2 -- Chapter 6- Modern Genetics
MS-SCI-LS-Unit 2 -- Chapter 6- Modern Genetics

... carried on the sex chromosomes. Genes on the X and Y chromosomes are often called sex-linked genes because their alleles are passed from parent to child on a sex chromosome. Traits controlled by sex-linked genes are called sex-linked traits. One sex-linked trait is red-green colorblindness. A person ...
mRNA
mRNA

... template strand and uses base pairing complementarity with the DNA template to create an RNA copy. Although RNA polymerase traverses the template strand from 3' → 5', the coding (non-template) strand and newly-formed RNA can also be used as reference points, so transcription can be described as occu ...
Molecular Basis of Heredity--ST03 1.2.7
Molecular Basis of Heredity--ST03 1.2.7

... Systems Approach--ST01, STI02, STI03, & STI04 1. Analyze systems, including inputs and outputs, as well as subsystems. Structure and Organization of Living Systems--ST03 1.2.6 6. Understand that specific genes regulate the functions performed by structures within the cells of multi-cellular organism ...
TRIDIA™ In Vitro Diagnostic Surfaces
TRIDIA™ In Vitro Diagnostic Surfaces

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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