Polymerase chain reaction (PCR) The polymerase chain reaction
... amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a v ...
... amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a v ...
Cloning a -cateninY654E-fl-neo targeting vector
... within exon 13, to replace the original tyrosine (Y) residue into a glutamic acid (E). Next, both 5.2 and 3.3 kb wild type fragments, and the modified 3.3 kb fragment were cloned into the TNLOX1-3 vector. To enable specific removal of the PGK-Neo cassette at a later stage, we replaced the original N ...
... within exon 13, to replace the original tyrosine (Y) residue into a glutamic acid (E). Next, both 5.2 and 3.3 kb wild type fragments, and the modified 3.3 kb fragment were cloned into the TNLOX1-3 vector. To enable specific removal of the PGK-Neo cassette at a later stage, we replaced the original N ...
FANCE Antibody
... The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a ...
... The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a ...
Natural genetic transformation: prevalence, mechanisms
... subdivision) strongly prefer to take up DNA containing their own specific DNA uptake signal sequences (DUS or USS). Thus, it was shown more than twenty years ago that N. gonorrhoeae does not take up DNA from H. influenzae and viceversa [91]. Both the 12 bp neisserial DUS sequence and its 9e10 bp USS ...
... subdivision) strongly prefer to take up DNA containing their own specific DNA uptake signal sequences (DUS or USS). Thus, it was shown more than twenty years ago that N. gonorrhoeae does not take up DNA from H. influenzae and viceversa [91]. Both the 12 bp neisserial DUS sequence and its 9e10 bp USS ...
Are you ready for S317?
... In order to multiply, cells must grow and make a copy of their genome in a process known as DNA replication. They then undergo cell division to produce two daughter cells each containing one copy of the genome. Prokaryotes divide by the process of binary fission after replicating their DNA. The proc ...
... In order to multiply, cells must grow and make a copy of their genome in a process known as DNA replication. They then undergo cell division to produce two daughter cells each containing one copy of the genome. Prokaryotes divide by the process of binary fission after replicating their DNA. The proc ...
Analysis of a genomic segment of white spot syndrome virus of
... disease has also been observed in other invertebrate aquatic organisms, such as crab and crayfish, and has a major impact on the economy of the shrimp farming industry in South-east Asia (Flegel, 1997). Infected shrimps show strong signs of lethargy and a reddish coloration of the hepatopancreas (Ch ...
... disease has also been observed in other invertebrate aquatic organisms, such as crab and crayfish, and has a major impact on the economy of the shrimp farming industry in South-east Asia (Flegel, 1997). Infected shrimps show strong signs of lethargy and a reddish coloration of the hepatopancreas (Ch ...
DNA and Protein Concentration Measurements Using Fluorescence
... (Q33210) was used to measure protein in solution. All working solutions and standards were prepared per the instructions provided with each kit. Sufficient volumes were prepared to allow for measurements in a standard 1-cm quartz fluorescence cuvette. ...
... (Q33210) was used to measure protein in solution. All working solutions and standards were prepared per the instructions provided with each kit. Sufficient volumes were prepared to allow for measurements in a standard 1-cm quartz fluorescence cuvette. ...
Translation
... 1. Begin in the middle with the first letter of the codon 2. Go outward to the second letter in the codon 3. Go outward again to the third letter in the codon. ...
... 1. Begin in the middle with the first letter of the codon 2. Go outward to the second letter in the codon 3. Go outward again to the third letter in the codon. ...
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements
... Animation: Mutagenic Effects of 5BU 1. Chemical mutagens may be naturally occurring, or synthetic. They form different groups based on their mechanism of action: a. Base analogs depend upon replication, which incorpocates a base with alternate states (tautomers) that allow it to base pair in alterna ...
... Animation: Mutagenic Effects of 5BU 1. Chemical mutagens may be naturally occurring, or synthetic. They form different groups based on their mechanism of action: a. Base analogs depend upon replication, which incorpocates a base with alternate states (tautomers) that allow it to base pair in alterna ...
NCEA Level 2 Biology (91159) 2013
... Mutagens cause mutations / change in DNA sequence Environmental factors vary widely, but can include high / low / change in temperature, wind, salinity, available nutrients (food) etc. (Any reasonable / appropriate environmental factors can be accepted.) Mutagens cause changes to the genetic materia ...
... Mutagens cause mutations / change in DNA sequence Environmental factors vary widely, but can include high / low / change in temperature, wind, salinity, available nutrients (food) etc. (Any reasonable / appropriate environmental factors can be accepted.) Mutagens cause changes to the genetic materia ...
Transcription Translation PowerPoint
... 4. Every three nucleotides on mRNA is called a ____. (You may need to come back for this) ...
... 4. Every three nucleotides on mRNA is called a ____. (You may need to come back for this) ...
REVIEW 5 Heredity Modern society uses scientific knowledge to
... A characteristic in an organism: The structure into which DNA arranges itself: ...
... A characteristic in an organism: The structure into which DNA arranges itself: ...
Chloroplast DNA and Molecular Phylogeny
... deletions and additions) occurring only very rarely, small length mutations of a few bp to several hundred bp are relatively common during chloroplast genome evolution. The linear order and Introduction arrangement of chloroplast sequences The widespread availability of many is extraordinarily conse ...
... deletions and additions) occurring only very rarely, small length mutations of a few bp to several hundred bp are relatively common during chloroplast genome evolution. The linear order and Introduction arrangement of chloroplast sequences The widespread availability of many is extraordinarily conse ...
Gene prediction
... Prediction • Statistical: coding segments (exons) have typical sequences on either end and use different subwords than non-coding segments (introns). • Similarity-based: many human genes are similar to genes in mice, chicken, or even bacteria. Therefore, already known mouse, chicken, and bacterial g ...
... Prediction • Statistical: coding segments (exons) have typical sequences on either end and use different subwords than non-coding segments (introns). • Similarity-based: many human genes are similar to genes in mice, chicken, or even bacteria. Therefore, already known mouse, chicken, and bacterial g ...
Supplemental Text
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
Advanced Plant Technology Program Vocabulary
... Gene (noun): A section of the genetic code that determines a trait. Many sections of DNA do not determine a trait and are called “junk DNA”. Genetics (noun): The study of genes and their effects on individuals. Genome (noun): All of the genetic material an organism possesses. Genome-wide associati ...
... Gene (noun): A section of the genetic code that determines a trait. Many sections of DNA do not determine a trait and are called “junk DNA”. Genetics (noun): The study of genes and their effects on individuals. Genome (noun): All of the genetic material an organism possesses. Genome-wide associati ...
The application of molecular genetics to detection of
... level. There have been few definitive findings which hold up in more than a single model system, and any genetic or environmental factor that appears critical in one case can be excluded in another. The analysis of single gene mutations using RFLPs for linkage studies has had considerable success in ...
... level. There have been few definitive findings which hold up in more than a single model system, and any genetic or environmental factor that appears critical in one case can be excluded in another. The analysis of single gene mutations using RFLPs for linkage studies has had considerable success in ...
Prokaryotic DNA organization • Circular DNA • Condensed by packaging proteins
... inactivated by high temperatures • Taq, Vent polymerases isolated from thermophiles Fig,14.8 ...
... inactivated by high temperatures • Taq, Vent polymerases isolated from thermophiles Fig,14.8 ...
RNA
... The Structure of RNA There are four main differences between RNA and DNA: • The sugar in RNA is ribose instead of deoxyribose. • RNA is single-stranded. DNA is double-stranded. • RNA contains uracil in place of thymine. • DNA stays in the nucleus, but RNA can leave the nucleus and go into the cytopl ...
... The Structure of RNA There are four main differences between RNA and DNA: • The sugar in RNA is ribose instead of deoxyribose. • RNA is single-stranded. DNA is double-stranded. • RNA contains uracil in place of thymine. • DNA stays in the nucleus, but RNA can leave the nucleus and go into the cytopl ...
Scientists Say Human Genome Is Complete
... consortium's leaders said from the outset they would not try to do so. Within the rest of the DNA, known as euchromatic DNA, some regions are very hard to sequence for technical reasons. For example, they may contain DNA that is toxic to the bacteria used to amplify them. Foreseeing such difficult r ...
... consortium's leaders said from the outset they would not try to do so. Within the rest of the DNA, known as euchromatic DNA, some regions are very hard to sequence for technical reasons. For example, they may contain DNA that is toxic to the bacteria used to amplify them. Foreseeing such difficult r ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.