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Chem 499 Final Exam Name
Chem 499 Final Exam Name

... 15. Cytochrom P-450 enzymes use iron in different oxidation states to change the oxidation state of many functional groups in biochemistry. “Fe+3“ (reduced) and “Fe+4-O“ (oxidized) show simplified representations of these enzymes that are commonly used. O ...
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ATP production in brain and liver mitochondria of Fischer
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... temperature of the larger assay volume of the reaction mixture (990 ␮l), which is kept at 28°C. Like the standards, the background of the reaction mixtures (990 ␮l) was recorded before the addition of mitochondria. Afterward, 10 ␮l of freshly isolated mitochondria from the frontal cortex of the brai ...
Influence of temperature on the dynamics of ATP, ADP and non
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... ATP/ADP ratio did not occur until after the end of January (Figures 2 and 3). On the other hand, the dynamics of the changes shown in Figures 2 and 3 were consistent with results obtained from the one-node cutting test applied on buds of Redhaven trees cultivated under natural conditions over severa ...
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AMINO ACID METABOLISM

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A Strongly Inwardly Rectifying K+ Channel that Is Sensitive to ATP
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... All living things are characterised by levels of organisation that are hierarchical. The cell is the lowest level of organisation that can exist independently. Multicellular organisms, like humans, have cells organised into groups of cells called tissues, the next level of organisation. Tissues are ...
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Glycolysis



Glycolysis (from glycose, an older term for glucose + -lysis degradation) is the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+. The free energy released in this process is used to form the high-energy compounds ATP (adenosine triphosphate) and NADH (reduced nicotinamide adenine dinucleotide).Glycolysis is a determined sequence of ten enzyme-catalyzed reactions. The intermediates provide entry points to glycolysis. For example, most monosaccharides, such as fructose and galactose, can be converted to one of these intermediates. The intermediates may also be directly useful. For example, the intermediate dihydroxyacetone phosphate (DHAP) is a source of the glycerol that combines with fatty acids to form fat.Glycolysis is an oxygen independent metabolic pathway, meaning that it does not use molecular oxygen (i.e. atmospheric oxygen) for any of its reactions. However the products of glycolysis (pyruvate and NADH + H+) are sometimes disposed of using atmospheric oxygen. When molecular oxygen is used in the disposal of the products of glycolysis the process is usually referred to as aerobic, whereas if the disposal uses no oxygen the process is said to be anaerobic. Thus, glycolysis occurs, with variations, in nearly all organisms, both aerobic and anaerobic. The wide occurrence of glycolysis indicates that it is one of the most ancient metabolic pathways. Indeed, the reactions that constitute glycolysis and its parallel pathway, the pentose phosphate pathway, occur metal-catalyzed under the oxygen-free conditions of the Archean oceans, also in the absence of enzymes. Glycolysis could thus have originated from chemical constraints of the prebiotic world.Glycolysis occurs in most organisms in the cytosol of the cell. The most common type of glycolysis is the Embden–Meyerhof–Parnas (EMP pathway), which was discovered by Gustav Embden, Otto Meyerhof, and Jakub Karol Parnas. Glycolysis also refers to other pathways, such as the Entner–Doudoroff pathway and various heterofermentative and homofermentative pathways. However, the discussion here will be limited to the Embden–Meyerhof–Parnas pathway.The entire glycolysis pathway can be separated into two phases: The Preparatory Phase – in which ATP is consumed and is hence also known as the investment phase The Pay Off Phase – in which ATP is produced.↑ ↑ 2.0 2.1 ↑ ↑ ↑ ↑ ↑ ↑
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