Practical Aspects of Estimating Energy Components in
... High expression of SAA in carcinomas was detected mostly in tumor cells, but not in normal mucosa. AG490, inhibitor of STAT3 activation, reduced SAA1 expression in SAS cells. SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway ...
... High expression of SAA in carcinomas was detected mostly in tumor cells, but not in normal mucosa. AG490, inhibitor of STAT3 activation, reduced SAA1 expression in SAS cells. SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway ...
Herculex® I Product Overview
... or purified Bt proteins, have been used safely in spray form as a crop protective agent for at least 40 years (Schnepf et al., 1998; U.S. EPA, 1996). The ssp. aizawai is used commercially to control wax moth larvae and various caterpillars (Cornell University, 1996). In its natural form found in the ...
... or purified Bt proteins, have been used safely in spray form as a crop protective agent for at least 40 years (Schnepf et al., 1998; U.S. EPA, 1996). The ssp. aizawai is used commercially to control wax moth larvae and various caterpillars (Cornell University, 1996). In its natural form found in the ...
University of Groningen Cross-linking of dimeric CitS and GltS
... described before (Dobrowolski et al. 2007, Dobrowolski and Lolkema 2009). Plasmid pGltS-BAD1 was obtained by ligation of the BAD encoding fragment with NcoI overhangs into the NcoI site of pN356C situated in between the His-tag and GltS encoding sequences. Plasmid pGltS-BAD206 was obtained in a simi ...
... described before (Dobrowolski et al. 2007, Dobrowolski and Lolkema 2009). Plasmid pGltS-BAD1 was obtained by ligation of the BAD encoding fragment with NcoI overhangs into the NcoI site of pN356C situated in between the His-tag and GltS encoding sequences. Plasmid pGltS-BAD206 was obtained in a simi ...
BIOL 1322 General Nutrition
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... the overexpressed lens version of a gecko housekeeping aldose reductase, because B-crystallin does not display the characteristic aldose reductase activity (see below). It thus may have arisen by gene duplication of a functional aldose reductase gene. If so, this duplication likely has been a relat ...
... the overexpressed lens version of a gecko housekeeping aldose reductase, because B-crystallin does not display the characteristic aldose reductase activity (see below). It thus may have arisen by gene duplication of a functional aldose reductase gene. If so, this duplication likely has been a relat ...
BIOL 1322 General Nutrition
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
Cloning and functional expression of invertebrate connexins from
... 45%, but still did not indicate an orthologous human connexin (Fig. 3A). In contrast, alignment of the second conserved 80 amino acid region comprising the third through the fourth transmembrane domains (M3–M4, Fig. 3B) showed a more variable level of homology with the lowest pairwise identity to C ...
... 45%, but still did not indicate an orthologous human connexin (Fig. 3A). In contrast, alignment of the second conserved 80 amino acid region comprising the third through the fourth transmembrane domains (M3–M4, Fig. 3B) showed a more variable level of homology with the lowest pairwise identity to C ...
Iron mediated methylthiolation of tRNA as a regulator of operon
... extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles per 150 pi volume. The results were consistent in that extracts from the ovotransferrin grown cells were always slightly more active with MS2 RNA than those from the iron r ...
... extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles per 150 pi volume. The results were consistent in that extracts from the ovotransferrin grown cells were always slightly more active with MS2 RNA than those from the iron r ...
Document
... Multiple Sequence Alignment tools are used to compare the amino acid sequences of more than two proteins. The word-size is the length of the seed set of amino acids, which needs to match exactly to get extended in both directions. Window Length is the length of the residues on either side, till whic ...
... Multiple Sequence Alignment tools are used to compare the amino acid sequences of more than two proteins. The word-size is the length of the seed set of amino acids, which needs to match exactly to get extended in both directions. Window Length is the length of the residues on either side, till whic ...
Protein Feature Identification
... • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, location, 2o structur ...
... • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, location, 2o structur ...
Goal - Cytoscape Wiki
... • Method – Calculate z-score/node, ZA score/subgraph, correct vs. random expression data sampling – Score over multiple experimental conditions – Simulated annealing used to find high scoring networks Ideker T, Ozier O, Schwikowski B, Siegel AF Bioinformatics. 2002;18 Suppl 1:S233-40 ...
... • Method – Calculate z-score/node, ZA score/subgraph, correct vs. random expression data sampling – Score over multiple experimental conditions – Simulated annealing used to find high scoring networks Ideker T, Ozier O, Schwikowski B, Siegel AF Bioinformatics. 2002;18 Suppl 1:S233-40 ...
Next Generation Quantitative PCR
... as SYBR Green Dye, or special probes such as TaqMan probe. abm selection of EvaGreen qPCR MasterMixes and TaqProbe qPCR MasterMixes provides various options for both methods which also accommodates most qPCR thermal instrument models available on the market. ...
... as SYBR Green Dye, or special probes such as TaqMan probe. abm selection of EvaGreen qPCR MasterMixes and TaqProbe qPCR MasterMixes provides various options for both methods which also accommodates most qPCR thermal instrument models available on the market. ...
Hypoxia in skeletal muscles: from physiology to gene expression
... of Erythropoietin (EPO), which is required for the formation of red blood cells, can be another contributor for the angiogenesis during hypoxia [71]. Additionally, hypoxia has been shown to increase the rate of collagen synthesis. Exposure of mice to hypoxia induced up-regulation of collagen type I ...
... of Erythropoietin (EPO), which is required for the formation of red blood cells, can be another contributor for the angiogenesis during hypoxia [71]. Additionally, hypoxia has been shown to increase the rate of collagen synthesis. Exposure of mice to hypoxia induced up-regulation of collagen type I ...
Differential scanning calorimetry as tool in observing thermal and
... Introduction The mechanisms of protein degradation by chemical or physical means have been extensively reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure ...
... Introduction The mechanisms of protein degradation by chemical or physical means have been extensively reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure ...
DOC-file of additional text
... IV. Receiver Operating Characteristic Curve (ROC) method Two values were assigned to each E. coli metabolic gene: a binary variable representing presence or absence of the gene in a given Buchnera genome, and the number of occurrences of the gene in 500 simulated reduced genomes. For each cut-off ( ...
... IV. Receiver Operating Characteristic Curve (ROC) method Two values were assigned to each E. coli metabolic gene: a binary variable representing presence or absence of the gene in a given Buchnera genome, and the number of occurrences of the gene in 500 simulated reduced genomes. For each cut-off ( ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Diese Dissertation wurde im Sinne von §13 Abs. 3 bzw. 4 der Promotionsordnung vom 29. Januar 1998 von Herrn Prof. Dr. Dieter Oesterhelt betreut. ...
... Diese Dissertation wurde im Sinne von §13 Abs. 3 bzw. 4 der Promotionsordnung vom 29. Januar 1998 von Herrn Prof. Dr. Dieter Oesterhelt betreut. ...
Get - Wiley Online Library
... are planar. Although several tautomeric forms exist in principle, the keto and amino forms predominate (Figure 2). Typically, the enol and imino forms are no more than 0.1% of the total, although this amount can change dramatically if modified bases are considered or if base pairs are formed. Details ...
... are planar. Although several tautomeric forms exist in principle, the keto and amino forms predominate (Figure 2). Typically, the enol and imino forms are no more than 0.1% of the total, although this amount can change dramatically if modified bases are considered or if base pairs are formed. Details ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... method to scan the Saccharomyces cerevisiae ORFs for potential peroxisomal proteins. Including both PTS1 and PTS2 motifs in their search, they found 18 new potential peroxisomal proteins. GFP fusions allowed them to confirm that about half of these proteins were truly located in the peroxisome. Anot ...
... method to scan the Saccharomyces cerevisiae ORFs for potential peroxisomal proteins. Including both PTS1 and PTS2 motifs in their search, they found 18 new potential peroxisomal proteins. GFP fusions allowed them to confirm that about half of these proteins were truly located in the peroxisome. Anot ...
Lecture 13 Electrophoresis (Part-I)
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
Focus Issue on Plastid Biology Update Novel
... (FNR) to NADP+ generating NADPH + H+. By this means the electrons cross a redox potential difference of about 1.13 V in total which is strong enough to fuel all subsequent redox-dependent reactions in the cell. The reducing power delivered from the electron transport chain is distributed to mainly t ...
... (FNR) to NADP+ generating NADPH + H+. By this means the electrons cross a redox potential difference of about 1.13 V in total which is strong enough to fuel all subsequent redox-dependent reactions in the cell. The reducing power delivered from the electron transport chain is distributed to mainly t ...
Characterization of microbial activity in the surface layers of a
... that the abiotic adsorption is constant with time. Studies with adenine showed that this is not strictly the case; however, adsorption was rapid (on the order of seconds) and subsequent increases were slight. 3HDNA measurements, on the other hand, were unaffected by the adsorbed adenine and hence no ...
... that the abiotic adsorption is constant with time. Studies with adenine showed that this is not strictly the case; however, adsorption was rapid (on the order of seconds) and subsequent increases were slight. 3HDNA measurements, on the other hand, were unaffected by the adsorbed adenine and hence no ...
Cytochromes P450 – importance of tissue specificity
... At first it was believed that all cytochromes P450 are ER resident proteins that are retained directly in the ER and do not undergo recycling through the pre-Golgi compartment [12, 13]. However, later studies on some cytochromes P450 showed this is not always the case. Lanosterol 14α-demetylase (CYP ...
... At first it was believed that all cytochromes P450 are ER resident proteins that are retained directly in the ER and do not undergo recycling through the pre-Golgi compartment [12, 13]. However, later studies on some cytochromes P450 showed this is not always the case. Lanosterol 14α-demetylase (CYP ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... to an uronic acid. GAGs reside both in the ECM (extracellular matrix) and at the cell surface as constituents of proteoglycans. These sugars, apart from having important structural roles in the ECM, are also fundamental modulators of many biological processes, such as development, cell proliferation ...
... to an uronic acid. GAGs reside both in the ECM (extracellular matrix) and at the cell surface as constituents of proteoglycans. These sugars, apart from having important structural roles in the ECM, are also fundamental modulators of many biological processes, such as development, cell proliferation ...
STRIVE Report Series No.65
... P. putida CA-3 not only offers a significant styrene remediation capability but also the opportunity to use a toxic waste compound as the starting material for the production of value-added environmentally friendly bio-plastic. This project has focused mainly on the styrene-degrading ability of P. p ...
... P. putida CA-3 not only offers a significant styrene remediation capability but also the opportunity to use a toxic waste compound as the starting material for the production of value-added environmentally friendly bio-plastic. This project has focused mainly on the styrene-degrading ability of P. p ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.