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University of Groningen Transport processes in penicillin
University of Groningen Transport processes in penicillin

... improvement of strains and cultivation conditions is only practical when factors that limit the yield and rate of penicillin production can be pinpointed and removed easily. In recent studies on prospects of metabolically engineering penicillin production, two general approaches have been applied to ...
bile acids - The Vespiary
bile acids - The Vespiary

Synthesis of 2º Alcohols Grignard + aldehyde yields a secondary alcohol. =>
Synthesis of 2º Alcohols Grignard + aldehyde yields a secondary alcohol. =>

... O-H, N-H, S-H, or -C—C-H. Grignard reagent is destroyed, becomes an alkane. • No other electrophilic multiple bonds, like C=N, C—N, S=O, or N=O. ...
Invariant amino acids essential for decoding function of polypeptide
Invariant amino acids essential for decoding function of polypeptide

... amino acids are very different from the initial Met residue and at the same time distinct among themselves, it implies that the eRF1 function is tolerant to the nature of amino acid in position 51. In a sharp contrast with Met51, position 55 is occupied exclusively by Glu (Figure 1). This may point ...
Module 3
Module 3

... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... In liquid microaerobic cultures without glutamate (N2fixing conditions), GDH, GOT, GTP, or all three were also much more active in A. lipoferum and A. amazonense than in A. brasiliense (data not shown). DISCUSSION Azospirillum spp. differ not only in the utilization of various carbon sources (23, 26 ...
The Bacterial Toxin RelE Displays Codon
The Bacterial Toxin RelE Displays Codon

... reduced the fraction of cells that could form colonies on solid medium plates (Christensen et al., 2001). Subsequent blocking of transcription of relE and overexpression of relB restored protein synthesis and colony formation (Pedersen et al., 2002). When an E. coli cell extract was first preincubat ...
Protein Sequencing and Identification With Mass
Protein Sequencing and Identification With Mass

... Peptide Identification Problem: Challenge Very similar peptides may have very different spectra! Goal: Define a notion of spectral similarity that correlates well with the sequence similarity. If peptides are a few mutations/modifications apart, the spectral similarity between their spectra should b ...
allyl cysteine sulphoxide
allyl cysteine sulphoxide

...  In initial experiments both undifferentiated and differentiating callus have been maintained for up to 15 days on a phytogel/MS medium, with and without sulphate, containing a range of potential precursors to the synthesis of Alliin (at different concentrations).  This method of substrate feeding ...
Current understanding of fatty acid biosynthesis and the acyl carrier
Current understanding of fatty acid biosynthesis and the acyl carrier

Module 3 Metabolism of carbohydrates, lipids
Module 3 Metabolism of carbohydrates, lipids

... C. * Binds tightly to lipoamide sulfur atoms D. All of the above E. None of the above 17. ATP is a cosubstrate of the enzyme PFK-1. In most species ATP is also an inhibitor of PFK-1 at higher concentrations. This seems to violate Le Chatelier's Principle. Which statement below would provide a suita ...
Purification and Physico-Chemical Analysis of Fractions from the
Purification and Physico-Chemical Analysis of Fractions from the

... toxicity of Ribi's endotoxin and produced a parallel decrease in the average sedimentation coefficient of the solute, although the 'acid hapten' obtained by this process (s = 1.4 S) retained certain antigenic characteristics of the parent endotoxin (Ribi et al. 1962). By using non-hydrolytic conditi ...
Novel Ciliate Genetic Code Variants Including the Reassignment of
Novel Ciliate Genetic Code Variants Including the Reassignment of

... Most organisms have either 1 or 2 stop codons reassigned to amino acids. It is also clear that evolution of TAA and TAG codon meanings is coupled, i.e., if one of these codons is reassigned the other codon is also reassigned. This is most likely because these two codons differ at the wobble position ...
article in press
article in press

... After completion of IVM, the oocytes were stripped of surrounding cumulus cells by repeated pipetting with a narrow-bore glass pipette in Hepes–SOF, and washed three times in Mg2+ /Ca2+ free PBS containing 1 mg/ml PVP. For each replicate, pools of ≥50 oocytes in 10 ␮l of PBS from each treatment were ...
the significance of hydrogen bonding
the significance of hydrogen bonding

Identification of a Chloroplast-encoded 9-kDa
Identification of a Chloroplast-encoded 9-kDa

... that centers B and A are iron-sulfur centers (4-7). Based on microwave power saturation studies, these two centers were further assigned as [4Fe-4S] clusters (8).The ESR spectrum of center X also had someresemblance to thoseof iron-sulfur centers (9), and Mossbauer spectroscopy suggestedthat center ...
N-terminal portion acts as an initiator of the inactivation of pepsin at
N-terminal portion acts as an initiator of the inactivation of pepsin at

... The most obvious differences between pepsin and pepsinogen are found in the prosegment portion. Not only does the prosegment cover the active site cleft, but it contains a large number of positively charged residues, i.e. 13 (nine Lys, two Arg, two His) out of the 44 residues in the prosegment of pe ...
David Rittenberg - National Academy of Sciences
David Rittenberg - National Academy of Sciences

... from their natural analogs by the methods commonly used. Nevertheless, the presence of the isotope made it possible to trace their metabolic fate. Thus, if a 2H-containing compound, B, was isolated after feeding the 2H-labeled compound, A, to an animal, the metabolic conversion A → B was established ...
Module 2 Biochemical bases of proliferation, intercellular
Module 2 Biochemical bases of proliferation, intercellular

... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
department of biological sciences
department of biological sciences

... mutagenesis. Different mutagens are used to induce mutation in parental strains and mutants of Aspergillus niger are used for commercial production. Hyper producer strains can be obtained by using ultra violet radiations and chemical mutagens together. Different methods of fermentation used can prod ...
Environmental enteric dysfunction is associated with carnitine
Environmental enteric dysfunction is associated with carnitine

... palmitoyltransferase II. Carnitine is then free to cycle back to the cytosol through the transporter (Fig. 1). Secondary carnitine deficiency is characterized by increases in serum acylcarnitines, fatty acid intermediates associated with blocked β-oxidation, and dicarboxylic acids produced by ω-oxida ...
Lactobacillus sanfrancisco a key sourdough lactic acid bacterium: a
Lactobacillus sanfrancisco a key sourdough lactic acid bacterium: a

... use substrates that are otherwise non-fermentable, and increase the microbial adaptability to difficult ecosystems. Under the influence of several ecological factors the homoand heterofermentative LAB have a great aptitude for producing metabolites other than lactic acid (Ceslovszky et al. 1992) and ...
Metabolism of [14C]glutamate and [14C]glutamine by
Metabolism of [14C]glutamate and [14C]glutamine by

Vitamins and Coenzymes
Vitamins and Coenzymes

... enzymatic reaction rather than the enolate • Conversion of the b-carbonly group into a protonated imine also facilitates the decarboxylation • The pH of an imine is near 7, so that under biochemical conditions the imine-nitrogen can be positively charged and acts as a very effective electron sink ...
105 ACID - DW Brooks
105 ACID - DW Brooks

... when they react with certain metals (like iron, zinc, and aluminum), and neutralize bases. Bases taste bitter (like soap), feel slippery to the touch, cause red litmus to turn blue, and neutralize acids. 2. Conceptual Definitions. Acids and bases can be defined conceptually to help account for what ...
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Peptide synthesis

In organic chemistry, peptide synthesis is the production of peptides, which are organic compounds in which multiple amino acids are linked via amide bonds, also known as peptide bonds. The biological process of producing long peptides (proteins) is known as protein biosynthesis.
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