Amino Acids in Maturation Medium and Presence
... when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed oocytes were cultured for 24-48 h in modified Whitten's medium (pH 7.4) supplemented with 10% porcine follicular fluid, 10 IU eCG/ml, and 10 IU hCG/ml (designated mWM-FG), nuclear maturation of oocy ...
... when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed oocytes were cultured for 24-48 h in modified Whitten's medium (pH 7.4) supplemented with 10% porcine follicular fluid, 10 IU eCG/ml, and 10 IU hCG/ml (designated mWM-FG), nuclear maturation of oocy ...
Conformational changes in actin–myosin isoforms probed by Ni(II
... Crucial information concerning conformational changes that occur during the mechanochemical cycle of actin– myosin complexes is lacking due to the difficulties encountered in obtaining their three-dimensional structures. To obtain such information, we employed a solution-based approach through the re ...
... Crucial information concerning conformational changes that occur during the mechanochemical cycle of actin– myosin complexes is lacking due to the difficulties encountered in obtaining their three-dimensional structures. To obtain such information, we employed a solution-based approach through the re ...
Document
... Synthesized in the liver Stored & concentrated in the gallbladder Discharged into gut and aides in absorption of intraluminal lipids, cholesterol, & fat soluble vitamines • Bile acid refers to the protonated form while bile salts refers to the ionized form – The pH of the intestine is 7 and the pKa ...
... Synthesized in the liver Stored & concentrated in the gallbladder Discharged into gut and aides in absorption of intraluminal lipids, cholesterol, & fat soluble vitamines • Bile acid refers to the protonated form while bile salts refers to the ionized form – The pH of the intestine is 7 and the pKa ...
Chemical polymerization of aniline in phenylphosphinic
... involves the quinoid nucleus having two imine nitrogens with pKa1 = 1.05 and pKa2 = 2.55,6 therefore any acid is suitable as a dopant if its pKa value lies in this range. Any acid having a pKa around the value of the anilinium ion (pKa = 4.60) would be suitable as the solvent and prevents overoxidat ...
... involves the quinoid nucleus having two imine nitrogens with pKa1 = 1.05 and pKa2 = 2.55,6 therefore any acid is suitable as a dopant if its pKa value lies in this range. Any acid having a pKa around the value of the anilinium ion (pKa = 4.60) would be suitable as the solvent and prevents overoxidat ...
Case Study: BPTI
... formed by the internal packing of non-polar amino acid side chains. BPTI is no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimensional structure is provided by the three internal disulfide bonds that w ...
... formed by the internal packing of non-polar amino acid side chains. BPTI is no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimensional structure is provided by the three internal disulfide bonds that w ...
The Endogenous Citric Acid-Cycle Intermediates and Amino Acids
... fumarate, malate and pyruvate) increased the endogenous 02 uptake; the maximum Q02 values were given with a-oxoglutarate (Kg), succinate (S), pyruvate (Py) or malate (Ma). H, Homogenates; M, mitochondria. ...
... fumarate, malate and pyruvate) increased the endogenous 02 uptake; the maximum Q02 values were given with a-oxoglutarate (Kg), succinate (S), pyruvate (Py) or malate (Ma). H, Homogenates; M, mitochondria. ...
Amino Acid Catabolism: C
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
Amino Acid Catabolism: C
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
(LPT1) and humans (LPCAT3)
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
1. Fatty acids may be synthesized from dietary glucose via pyruvate
... (TE). The flexible phosphopantetheinyl group (green) carries the fatty acyl chain from one catalytic site on a chain to another, as well as between chains in the dimer. ...
... (TE). The flexible phosphopantetheinyl group (green) carries the fatty acyl chain from one catalytic site on a chain to another, as well as between chains in the dimer. ...
To remember Sir Hans Krebs: Nobelist, Friend, and Adviser
... leads to the re-formation of oxaloacetic acid, but allows packets of electrons to be released in small steps that can be strictly controlled. Figure 1 presents an illustration of the Krebs cycle. As Krebs explained in his Nobel prize lecture, “Of major significance was another new observation. Citra ...
... leads to the re-formation of oxaloacetic acid, but allows packets of electrons to be released in small steps that can be strictly controlled. Figure 1 presents an illustration of the Krebs cycle. As Krebs explained in his Nobel prize lecture, “Of major significance was another new observation. Citra ...
Biologically Active Oxylipins from Enzymatic and Nonenzymatic
... and cymatherols [18,22,23,52]. Although extensive detail about oxylipin occurrence in microalgae is beyond this review, some important features of oxylipin metabolism in these unicellular organisms cannot be discarded. A characteristic difference from macroalgae is the complete absence of C18 PUFA-d ...
... and cymatherols [18,22,23,52]. Although extensive detail about oxylipin occurrence in microalgae is beyond this review, some important features of oxylipin metabolism in these unicellular organisms cannot be discarded. A characteristic difference from macroalgae is the complete absence of C18 PUFA-d ...
Fatty Acid Biosynthesis
... highly reactive nucleophile that attacks the carbonyl of butyryl-S-KSase. This cycle continues until palmitoyl ACP is formed. The thioester bond is hydrolyzed by thioesterase to form palmitate. V. Elongation of Palmitate Palmitate is the primary product of fatty acid synthase. Fatty acids with short ...
... highly reactive nucleophile that attacks the carbonyl of butyryl-S-KSase. This cycle continues until palmitoyl ACP is formed. The thioester bond is hydrolyzed by thioesterase to form palmitate. V. Elongation of Palmitate Palmitate is the primary product of fatty acid synthase. Fatty acids with short ...
The proofreading mechanism of isoleucyl
... (mRNA) encoding a protein. For an average gene of 1000 basepairs this means that the polymerase must pick out and incorporate the correct ribonucleoside‐ triphosphate 1000 times. In the following protein biosynthesis, the translation, the ribosome catalyses the polymerisation of ...
... (mRNA) encoding a protein. For an average gene of 1000 basepairs this means that the polymerase must pick out and incorporate the correct ribonucleoside‐ triphosphate 1000 times. In the following protein biosynthesis, the translation, the ribosome catalyses the polymerisation of ...
metabolism - Garland Science
... conditions increases the potential for metabolic flexibility and diversity. First, it allows metabolic reactions requiring very different conditions to occur simultaneously in the same cell. Second, it allows pathways and reactions that take place in more than one compartment to proceed with opposit ...
... conditions increases the potential for metabolic flexibility and diversity. First, it allows metabolic reactions requiring very different conditions to occur simultaneously in the same cell. Second, it allows pathways and reactions that take place in more than one compartment to proceed with opposit ...
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia
... amino acid present in each of the two agegroups studied, and also allowed the quantitative estimation of the differences between the amino acids stored, always related to the actual empty body weight of the animal and referred to the age of comparison. The known amount of each food item consumed dai ...
... amino acid present in each of the two agegroups studied, and also allowed the quantitative estimation of the differences between the amino acids stored, always related to the actual empty body weight of the animal and referred to the age of comparison. The known amount of each food item consumed dai ...
Synthesis of novel dendrimers having aspartate grafts and their
... drugs (NSAIDs) with a functional group –COOH (pKa ¼ 4.2), so its solubility in water is pH-dependent [11]. Because the acidity of naproxen, the inherent solubility was 1.18 mg/ml, pH ¼ 6; 2.24 mg/ ml, pH ¼ 7; 24.45 mg/ml, pH ¼ 8. We measured the solubility of naproxen in the presence of T1–T4 dendri ...
... drugs (NSAIDs) with a functional group –COOH (pKa ¼ 4.2), so its solubility in water is pH-dependent [11]. Because the acidity of naproxen, the inherent solubility was 1.18 mg/ml, pH ¼ 6; 2.24 mg/ ml, pH ¼ 7; 24.45 mg/ml, pH ¼ 8. We measured the solubility of naproxen in the presence of T1–T4 dendri ...
Increased Lysine Synthesis Coupled with a Knockout of
... of free Thr were found (Figure 7A). By contrast, a positive correlation between free Met and free Lys was evident in group 1, but no correlation was obtained between these two free amino acids in group 2, in which relatively high levels of free Met were found (Figure 7B). The lack of correlation bet ...
... of free Thr were found (Figure 7A). By contrast, a positive correlation between free Met and free Lys was evident in group 1, but no correlation was obtained between these two free amino acids in group 2, in which relatively high levels of free Met were found (Figure 7B). The lack of correlation bet ...
Gene Section GAST (gastrin) Atlas of Genetics and Cytogenetics
... arginine 73-arginine 74 sequences, releasing the Cterminal flanking peptide, and generating G34. The post-cleavage residual basic residues are then removed by carboxypeptidase E, generating what are commonly referred to as the glycine-extended gastrins (i.e., G34Glycine). In contrast to PC1/3, PC2 i ...
... arginine 73-arginine 74 sequences, releasing the Cterminal flanking peptide, and generating G34. The post-cleavage residual basic residues are then removed by carboxypeptidase E, generating what are commonly referred to as the glycine-extended gastrins (i.e., G34Glycine). In contrast to PC1/3, PC2 i ...
05 Farm Animal Metabolism 05
... by intestinal cells of most species are apoB48, apo-AI and apo-AIV. The resulting particles, called chylomicrons, are quite large in mammalian species (50–500 nm) and contain by weight 85–95% triacylglycerol, 4–9% phospholipids, ~1% free cholesterol, ~0.5% esterified cholesterol and ~0.6% protein (B ...
... by intestinal cells of most species are apoB48, apo-AI and apo-AIV. The resulting particles, called chylomicrons, are quite large in mammalian species (50–500 nm) and contain by weight 85–95% triacylglycerol, 4–9% phospholipids, ~1% free cholesterol, ~0.5% esterified cholesterol and ~0.6% protein (B ...
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... Headquartered in Asheville, N.C., Genova Diagnostics is a global specialty clinical laboratory, pioneering a systems approach that supports healthcare providers in the personalized treatment and prevention of chronic disease. Chronic diseases are often complex and Genova’s system-based testing helps ...
... Headquartered in Asheville, N.C., Genova Diagnostics is a global specialty clinical laboratory, pioneering a systems approach that supports healthcare providers in the personalized treatment and prevention of chronic disease. Chronic diseases are often complex and Genova’s system-based testing helps ...
Effects of low crude-protein diets fortified with crystalline amino acids
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
