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Topic 1: Cell biology (15 hours)
Topic 1: Cell biology (15 hours)

... 2. The replication of DNA is semi-conservative and 10. Application: Use of Taq DNA polymerase to produce depends on complementary base pairing. multiple copies of DNA rapidly by the polymerase 3. Helicase unwinds the double helix and separates the chain reaction (PCR). two strands by breaking hydrog ...
DNA & RNA
DNA & RNA

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Roles of enzymes

... to break down appropriate nucleic ...
DNA and Protein Synthesis
DNA and Protein Synthesis

DNA, RNA and Protein
DNA, RNA and Protein

Chapter 14
Chapter 14

... How is RNA Transcribed from DNA? • Three classes of RNA • 1- Messenger RNA (mRNA) carries the blueprint for protein assembly to the ribosome (Goes in the nucleus and translates the material from DNA to RNA) • 2- Transfer RNA (tRNA) brings the correct amino acid to the ribosome and pairs up with ...
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Document

... ...
During DNA replication, which of the following segments would be
During DNA replication, which of the following segments would be

... D a mRNA strand with the sequence UUCGACCCU ...
Edible DNA Strand
Edible DNA Strand

... Red= A, pairs with Green = T Yellow = G pairs with Clear = C A pairs with T, G pairs with C! 1. Using toothpicks, connect “base pairs” of gummy bears (make sure they’re matched correctly!) 2. Use toothpicks to connect the “backbone” 3. Repeats steps 1 and 2. 4. Once 8-10 rungs of the “ladder” have b ...
Chromosomes Key - Iowa State University
Chromosomes Key - Iowa State University

... The coiling in question 3 is caused by what type of protein? _topoisomerase___ 4. Prokaryotic chromosomes are different than Eukaryotic chromosomes because: a) they are single stranded b) they are located in the nucleus c) they are circular 5. Explain the difference between a nucleosome and a chroma ...
DNA
DNA

Central dogma
Central dogma

... • All proteins consist of polypeptide chains – A linear sequence of amino acids • Each chain corresponds to the nucleotide base sequence of a gene ...
Central dogma
Central dogma

... All proteins consist of polypeptide chains ...
Genomics on the Web Handout
Genomics on the Web Handout

... investigate the following chapters by first selecting the chapter name, and then selecting the “Animation” tab at the bottom of the page. One you have completed the chapter’s animated tour describing people, discoveries, and concepts, complete the quiz by selecting the “problem” tab at the bottom of ...
posted
posted

... copy of a DNA sequence to site of protein synthesis at the ribosome • Transfer RNA (tRNA)—carries amino acids for polypeptide assembly • Ribosomal RNA (rRNA)—catalyzes peptide bond formation and provides structure for the ribosome ...
Chapter 9: DNA Structure and Analysis
Chapter 9: DNA Structure and Analysis

... not some other molecule, serves as the genetic material in bacteria, bacteriophages, and eukaryotes? • How do we know that the structure of DNA is in the form of a right-handed double helical model? • How do we know that in DNA, G pairs with C and A pairs with T as complementary strands are formed? ...
Concept 11-1 & 11-2
Concept 11-1 & 11-2

... The Double helix and Complementary Base Pairs p.231 -double helix: two strands of nucleotides wound about each other; structure of DNA ...
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Key

... Short answer Questions – you may continue your answer on a separate sheet of paper. 1. Use a DNA strand to DRAW and EXPLAIN how a strand of DNA would replicate. Why is it important for DNA to be able to make an exact copy (how does that ability help an organism live?)? DNA must be able to make an ex ...
lecture5
lecture5

... Excision Repair, in which the damaged base or bases are removed and then replaced with the correct ones in a localized burst of DNA synthesis. There are three modes of excision repair, each of which employs specialized sets of enzymes. Base Excision Repair (BER) Nucleotide Excision Repair (NER) Mism ...
DNA (Deoxyribonucleic Acid)
DNA (Deoxyribonucleic Acid)

... living organisms. ...
DNA - Lockland Schools
DNA - Lockland Schools

From DNA to Proteins
From DNA to Proteins

... DNA Strand: ...
GENE MUTATION = POINT MUTATION at the DNA level: at the level
GENE MUTATION = POINT MUTATION at the DNA level: at the level

... It may then be predicted that an E. coli cell, growing with a generation time of 40 min at 37degC, should lose 0.5 purine/chromosome in each generation. For a mammalian cell, which contains ~800 times more DNA than E. coli and grows with a generation time of 20 hr, 12,000 purines should be lost from ...
Syllabus Checklist
Syllabus Checklist

... protein synthesis involves the transcription of a gene on DNA into messenger ribonucleic acid (RNA) in the nucleus and translation into an amino acid sequence at the ribosome with the aid of transfer RNA. ...
Streptavidin is a small bacterial protein that binds
Streptavidin is a small bacterial protein that binds

... If the DNA has their own replication sequences and some gene (‘marker’) that allows the cells to survive under certain conditions, it can be maintained in the cells for many generations (as long as in the presence of such selective conditions). Moreover, DNA sequences can also be integrated into the ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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