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EOC Review Packet #2
EOC Review Packet #2

... 5. What is the process that allows DNA to make copies of itself before cell division? REPLICATION 6. Where in the cell does this take place? THE NUCLEUS 7. Write the Complement of this sequence : ATTCGTTCAG TAAGCAAGTC ...
Sc9 - a 3.1(teacher notes)
Sc9 - a 3.1(teacher notes)

... http://www.youtube.com/watch?v=cwfO6SzGaEg&feature=related ...
Bio 220 MiniQuiz 1
Bio 220 MiniQuiz 1

... _____1. Organic growth factors include amino acids and vitamins. _____2. Chemoautotrophs use the light from the sun as their primary energy source. _____3. Both chocolate agar and blood agar contain blood. _____4. Transcription refers to the process of DNA synthesis. Multiple choice _____5. An oblig ...
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dna - Kowenscience.com

Matko Chapter 10 Test Key
Matko Chapter 10 Test Key

... -uracil is used on the new strand instead of thymine -only one strand is being copied, not two -double helix DNA strand is the template -no ribosomes, tRNA, or polypeptide chains apparent ...
(DNA) polymerase I
(DNA) polymerase I

Molecular Genetics
Molecular Genetics

... - Gene regulation plays a role in determining structure and function of cells. - Both DNA regulatory sequences and regulatory genes are involved in gene expression. - In eukaryotes, gene expression is more complex and controlled by regulatory genes, regulatory elements, and transcription factors whi ...
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Teaching Notes

... helix, it is right handed and if you wrap the helix with your left hand then it is left handed. ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

IB Biology--Chromosome Review Activity
IB Biology--Chromosome Review Activity

DNA_and_Replication
DNA_and_Replication

... DNA is tightly coiled up around histone proteins so that it can all fit in the nucleus. The coiled DNA is called chromatin. ...
Ch. 10 DNA, RNA, PROTEIN SYNTHESIS
Ch. 10 DNA, RNA, PROTEIN SYNTHESIS

... – Step 3: DNA Polymerase finish creating replicating the 2 exposed strands and then falls off, resulting in 2 new DNA molecules. • Replication occurs at the replication fork in two separate directions at the same time; always in the 3’ to 5’ direction. – Replication errors are rare about 1 in 1 bill ...
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and

... deoxyadenosine 5’-triphosphate (dATP), inhibiting directly the DNA polymerases. Furthermore F-araATP is able to inhibit DNA primase, an accessory protein that synthesizes an RNA primer required for initiation of lagging strand synthesis by DNA polymerase. F-ara-ATP is also an effective inhibitor of ...
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Uses

... More Study Questions  PCR ...
Recombinant DNA Technology
Recombinant DNA Technology

...  We can amplify small target stretches of DNA by using the polymerase chain reaction (PCR)  This requires primers, Taq polymerase, and a thermocycler After cutting DNA, how do we separate the resulting fragments? Gel electrophoresis  But cutting complex DNA with restriction enzymes generates a hu ...
2014
2014

... A) It is located in the mitochondria. B) It is located in the nucleus. C) It uses NH4+ as a nitrogen source D) It uses glutamine as a nitrogen source Circle the correct answer 8. [2 points] Which of the following statements about topoisomerases is incorrect? A) Type II topoisomerases change the link ...
Basics Terms of Life Science Cells
Basics Terms of Life Science Cells

... • All living organisms contain cells, which are the fundamental working units of life. • A cell can be divided into two parts: the nucleus and the cytoplasm . ...
DNA From the Beginning WEBQUEST
DNA From the Beginning WEBQUEST

... Go to www.dnaftb.org and then complete the following web quest: Answer your questions on that document. Print when finished or email to Mrs. Berthelot: [email protected] Click on the link “Molecules of Genetics”, then click on Lesson 15 (DNA and proteins are key molecules of the cell nucleus) and ...
Chapter 13
Chapter 13

... • Consider the formula on page 406 for probability of finding a particular fragment in N clones • Suppose you seek a 99% probability of finding a given fragment in N clones of 10 kbp fragments • If your library is from the human genome, you would need 1,400,000 clones to reach 99% probability of fin ...
Document
Document

... An expression vector (i.e. plasmid) is engineered to contain regulatory sequences regions (e.g. promoter), an origin of replication, a selectable marker, and a suitable site for the insertion of a gene of interest such as the multiple cloning site. ...
Molecular Biology of the Gene
Molecular Biology of the Gene

... • The DNA genotype is expressed as proteins, which provides the molecular basis for phenotypic traits – DNA dictates the synthesis of proteins which determine the traits physically expressed by an organism ...
centromere
centromere

... Genome Organisation II • Eukaryotic genomes are completely different in their organisation compared to prokaryotic, and also much bigger • Their genes are mostly “split” into exons and introns • It is not certain which came first in evolution genes with introns/exons or genes without • Exons may all ...
Document
Document

... that corresponded to amino acids 1 through 10 of this protein was produced and labeled; it hybridized only to the 9 kb, 13 kb and 15 kb fragments. 6. True or false. The 3’ end of the mRNA made from this region would be located in the 2 kb restriction fragment. 7. True or false. These data indicate t ...
Molecular Biology
Molecular Biology

... • The DNA genotype is expressed as proteins, which provides the molecular basis for phenotypic traits – DNA dictates the synthesis of proteins which determine the traits physically expressed by an organism ...
DNA Manipulation
DNA Manipulation

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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