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old strand - TeacherWeb
old strand - TeacherWeb

DNA and RNA - Midway ISD
DNA and RNA - Midway ISD

... pairing. Each strand of the original DNA molecule is used as a template for the new DNA. ...
Ch. 16 The Molecular Basis of Life
Ch. 16 The Molecular Basis of Life

... in nucleotide excision repair: Nuclease = enzyme that cuts out the damaged DNA segment ­ DNA polymerase and ligase are responsible for filling the empty  spot back in. ex. for skin cells ultraviolet light can cause thymine dimers  = two  adjacent thymines that covalently link together and cause buc ...
Nucleic acids
Nucleic acids

... The three-dimensional structure of DNA was discovered in 1953 by Watson and Crick in Cambridge, using experimental data of Wilkins and Franklin in London, for which work they won a Nobel prize. The main features of the structure are: o DNA is double-stranded, so there are two polynucleotide stands a ...
Transcription PPT
Transcription PPT

CHAPTER OUTLINE
CHAPTER OUTLINE

... Deoxyribonucleic acid (DNA) is the genetic material of life, it is able to store information that pertains to the development, structure, and metabolic activities of the cell or organism and is stable so that it can be replicated with high accuracy during cell division and be transmitted from genera ...
12.1 and 12.2 Fill
12.1 and 12.2 Fill

... o genes had to be easily copied, because all of a cell’s genetic information is replicated every time a cell _____________ Scientists now wanted to know more about the molecule DNA. The Components and Structure of DNA DNA is a polymer (long chain) made of repeating subunits called ______________. Nu ...
Chapter 12 DNA
Chapter 12 DNA

... about which was the genetic material because proteins are more complex than DNA. • Finally in 1952 DNA was proven to be the genetic material. ...
DNA as the Genetic Material
DNA as the Genetic Material

... machine) called Okazaki fragments and then connecting them together B. Step by Step Replication 1. Starting at origins of replication along DNA molecules, Helicase enzymes untwist the double helix at its replication forks, separating the two old DNA strands 2. Then molecules of single-strand binding ...
Molecular_genetics_revision_checklist
Molecular_genetics_revision_checklist

... Functions, enzyme action (lock and key / induced fit) and factors that affect enzyme action. In terms of nucleotide, complementary bases, double helix structure. In terms of unwinding, base pair matching and semi conservative construction. Include the terms chromatid, chromosome and centromere, hist ...
DNA Basics - Thermo Fisher Scientific
DNA Basics - Thermo Fisher Scientific

... C pairs with G and T pairs with A There are only four molecules in the DNA chain: adenine (A), guanine (G), thymine (T) and cytosine (C). These As, Cs, Ts and Gs are also called “bases.” These four molecules partner: C partners with G and T partners with A. Pairing is a natural state for DNA and if ...
Types of DNA Mutations - Home
Types of DNA Mutations - Home

... Not normally present in DNA ...
L 17 _PCR
L 17 _PCR

... dye terminators: instead of radioactive dNTPs, use ddNTPs with fluorescent tags, a different color in each dideoxy reaction. Then all four reactions can be run on a single lane, with the colors read by a laser as each band runs off the bottom of the gel. automated sequencers use cycle sequencing (li ...
PCR - Polymerase Chain Reaction
PCR - Polymerase Chain Reaction

... you can get enough DNA from an environment) or from PCR product • T-RFLP (terminal-RFLP) is in most respects identical except for a marker on the end of the enzyme • Works as fingerprinting technique because different organisms with different DNA sequences will have different lengths of DNA between ...
Team Publications
Team Publications

... Martin Dutertre, Sarah Lambert, Aura Carreira, Mounira Amor-Guéret, Stéphan Vagner (2014 Mar ...
Document
Document

... 1. mRNA in the cytoplasm attaches to a ribosome 2. As each codon of the mRNA moves through the ribosome an amino acid is brought to the ribosome by tRNA Each tRNA molecule only carries one type of amino acid Each tRNA molecule also has 3 ...
Piecing Together an Identity
Piecing Together an Identity

... antiserum or lectin specific for its blood group substance then most of the antibody in the antiserum will bind to the blood group substance in the saliva. So when you add the red blood cells for that type no clumping or very little clumping should be observed. This is the opposite of what you would ...
to 3
to 3

... A. It can be used to analyze only DNA B. The heavier the fragment, the slower it moves C. The fragments of DNA are negatively charge and migrate to the positive pole D. A buffer must cover the gel to allow a current to pass through the system E. Restriction enzymes cut DNA in only certain sites on t ...
b8 nucleic acids
b8 nucleic acids

... of the five bases: adenine(A), cytosine(C), guanine(G), thymine(T) and uracil (U). Nucleic acids are joined by covalent bonds between the phosphate of one nucleotide and the sugar of the next, resulting in a backbone with a repeating pattern of sugar-phosphate-sugar-phospate. Nitrogenous bases are a ...
DNA and RNA Paper Lab Answer Key 1. deoxyribose C5H10O4
DNA and RNA Paper Lab Answer Key 1. deoxyribose C5H10O4

... present; formed from nucleotides; double stranded; remains in nucleus; contains a chemical message or code. RNA should be checked for ribonucleic acid; ribose present; phosphoric acid present; A, U, G, and C present; formed from nucleotides, single stranded, moves out of nucleus, contains a message ...
Ch. 10, DNA and Proteins
Ch. 10, DNA and Proteins

3.12 Translation
3.12 Translation

Electrical induction hypothesis to explain enhancer-promoter
Electrical induction hypothesis to explain enhancer-promoter

... The three‐dimensional conformation of chromosomes in the nucleus is important for many cellular processes, including the regulation of gene expression, DNA replication, and chromatin structure (Cremer and Cremer 2001). The technique of chromosome conformation capture (3C) evaluates long‐range intera ...
PPT
PPT

... • Factors affecting PCR – the concentrations of DNA polymerase, dNTPs, MgCl2, DNA and primers – the denaturing annealing and synthesis temperatures – the length and number of cycles – ramping times and the presence of contaminating DNA and inhibitors in the sample ...
Interest Grabber
Interest Grabber

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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