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Slide 1
Slide 1

... • At the replication fork, 1 daughter strand is continuously formed (leading strand) while the other (lagging strand) is formed in fragments, 1000b long, called Okazaki fragments. • DNA pol is the main replicating enzyme. Eukaryotes contain 4 types of DNA pol. ...
Strawberry DNA Extraction
Strawberry DNA Extraction

Valhalla High School
Valhalla High School

... Using the base pairing rules, find the anticodons for the template strand. A T C G TA G C Practice: Use these top strands of DNA and convert them into two strands. ...
Nucleotides - Mrs Miller's Blog | Science Revision
Nucleotides - Mrs Miller's Blog | Science Revision

... exposed by splitting the hydrogen bonds in the particular area of the DNA molecule • RNA nucleotides from a complementary strand (mRNA) which is a copy of the DNA coding strand (or gene) • The mRNA peels away from the DNA and leaves the nucleus through a nuclear pore • The mRNA attaches to a ribosom ...
Ch 20 Lecture
Ch 20 Lecture

No Slide Title
No Slide Title

... • double-stranded DNA composed of complementary strands • hydrogen bonds (weak) • determined by specific base pairing (A:T and G:C) • template for the synthesis • specific base-pairing permits detection and analysis of DNA/RNA ...
Gene Expression
Gene Expression

...  Signal molecule binding regulates ...
File
File

... As the helicase unwinds a portion of the DNA, the other portion of the molecule is stressed, due to the increased winding of the circular molecule. In order to relieve this stress, an enzyme called topoisomerase breaks and reattaches the DNA molecule. An enzyme, DNA polymerase, begins to match the f ...
Deoxyribonucleic Acid (DNA)
Deoxyribonucleic Acid (DNA)

... DNA and Chromosomes • __________: DNA located in _______ in form of ___________. – Number _______ widely between _______. – Humans = 46 chromosomes (23 pairs). ...
DNA sequencing - Winona State University
DNA sequencing - Winona State University

RFLPs, PCR, Gel Electrophoresis
RFLPs, PCR, Gel Electrophoresis

... will undergo PCR to make sure there is enough DNA for adequate testing. ...
Topic 11 DNA intro - Manhasset Public Schools
Topic 11 DNA intro - Manhasset Public Schools

... deoxyribonucleic acid. DNA is called a nucleic acid because it was first found in the nucleus. The DNA in the nucleus that actually controls the cell's workings. Heredity is the passing of genetic information from one generation to the next through reproduction. 6. What is a chromosome? 7. Where are ...
Transcription- lecture outline
Transcription- lecture outline

... mRNA is a single stranded molecule that typically has a very short halflife, in part because there are many very stable RNAases in most cells The process of making RNA from DNA is called transcription; Transcription requires the enzyme DNA-dependent RNA polymerase. The enzyme uses one strand, the te ...
Chapter 13: DNA Within the of almost all of your cells 46 DNA
Chapter 13: DNA Within the of almost all of your cells 46 DNA

... seen that they are able to fit together. It is possible for two hydrogen bonds to form between the _________________ negative on the nitrogen and oxygen atoms and the dipole positive on hydrogen atoms on the _________________ base. With base pairs C and G three hydrogen bonds can form between the ni ...
CHAPTER 10: DNA,RNA & Protein Synthesis
CHAPTER 10: DNA,RNA & Protein Synthesis

... • 3. Pyrimidines (T, C) single C ring ...
The ability to isolate plasmid DNA is crucial to recombinant DNA
The ability to isolate plasmid DNA is crucial to recombinant DNA

... In the alkaline lysis procedure, cells from the overnight culture are pelleted rapidly in a microcentrifuge and the pellet is resuspended in a buffered medium. Then the cells are lysed with a solution of SDS (Sodium dodecyl sulfate – a detergent that will denature proteins) and NaOH (sodium hydroxid ...
The ability to isolate plasmid DNA is crucial to recombinant DNA
The ability to isolate plasmid DNA is crucial to recombinant DNA

... In the alkaline lysis procedure, cells from the overnight culture are pelleted rapidly in a microcentrifuge and the pellet is resuspended in a buffered medium. Then the cells are lysed with a solution of SDS (Sodium dodecyl sulfate – a detergent that will denature proteins) and NaOH (sodium hydroxid ...
Nucleic acids - Sakshieducation.com
Nucleic acids - Sakshieducation.com

... In this process the DNA template transfers the genetic information in the form of code words to messenger RNA. This process is called as Transcription that helps in the synthesis of proteins. ...
Reverse Transcriptase and Retro Viruses
Reverse Transcriptase and Retro Viruses

... region (consists of a promoter and an operator) mRNA regulatory gene ...
Transcription - My Teacher Pages
Transcription - My Teacher Pages

... •Ribosomes begin attaching Amino Acids together to form proteins •Proteins enter into RER to be modified and folded (shaped) into structures •Vesicles form from RER and carry proteins to Golgi apparatus •There proteins are packaged, sorted and/ or stored in Golgi •Once the proteins are ...
DNA History and Replication
DNA History and Replication

Ms Gentry`s Nucleic acids powerpoint File
Ms Gentry`s Nucleic acids powerpoint File

... called mRNA (messenger) a copy of the original DNA (TRANSCRIPTION)  The mRNA peels away and leaves the nucleus through a nuclear pore and attaches to a ribosome  tRNA (transfer) brings amino acids to the ribosome in the correct order according to the base sequence on the mRNA (TRANSLATION)  The a ...
Chapter 17 - Madeira City Schools
Chapter 17 - Madeira City Schools

... 1. RNA Polymerase II binds to DNA and initiates transcription a. Promoter = is typically 25 nucleotides upstream from the starting point. This is where the TATA box (nucleotide sequence containing TATAAAA on the nontemplate strand) is. The promoter determines where RNA polymerase II starts and which ...
Review Sheet NYS Regents Lab Activity #1 Relationships and Biodiversity
Review Sheet NYS Regents Lab Activity #1 Relationships and Biodiversity

... 1. This lab has 7 tests used to determine the relatedness of 4 plant samples. Remember that scientists use a variety of evidence to determine evolutionary relationships, including cell types, structural morphology, DNA, behavior, embryology, and fossils. The more criteria that are shared between org ...
Review Questions and Answers Topic 1: Genes and DNA Copying
Review Questions and Answers Topic 1: Genes and DNA Copying

... Transfer RNA, or IRNA, carries specific amino acids to the ribosomes. Each IRNA molecule carries the amino acid that is specified by the molecule's anticodon. 5. What is the role of rRNA in gene expression? Ribosomal RNA, or rRNA, is part of the structure of ribosomes. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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