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Restriction Enzyme
Restriction Enzyme

... for Southern Blots : single-stranded DNA for Northern Blots : single-stranded mRNA ...
Protein Synthesis and Gene Expression Common Assessment Review
Protein Synthesis and Gene Expression Common Assessment Review

... 5. Which two enzymes are involved in DNA replication and what does each one do? a. Helicase—unwinds the double helix and breaks the hydrogen bonds (unwinds and unzips the DNA) b. DNA Polymerase—uses one side of the original DNA strand as template as well as base-pairing rules to make the second side ...
File
File

... DNA strand •The process by which information from a gene is used in the synthesis of a protein (a trait) •Exons – useful DNA (what is coded) •Introns – “Junk” DNA (what is non-coded) ...
DNA
DNA

... V. How exactly does DNA replicate? Several models for DNA replication were proposed: 1. Conservative model: Two completely new strands are formed, which coil together. Original strands stay together. ...
Chapter 10: Molecular Biology of the Gene
Chapter 10: Molecular Biology of the Gene

Problem Set 4B
Problem Set 4B

1. To research…
1. To research…

... (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA). The information in DNA is stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of ...
DNA
DNA

... Before the mRNA can go to the ribosome, it needs to be spliced. – The junk (parts of the DNA that are noncoding regions) called introns need to be cut out. – Exons (coding regions) are then stuck together. This is the correct concise message. ...
Plant disease - Topic exploration pack
Plant disease - Topic exploration pack

... structure of a leaf section. On the next page is a net diagram that can be folded into a cube. Using ‘help sheet 1’ (below), draw onto the net diagram the leaf section and then fold into a cube, using the tabs. ...
Drag and Drop Protein Synthesis Name Period Type in the following
Drag and Drop Protein Synthesis Name Period Type in the following

... correct answers below 1.Transcription is the first step of Protein Synthesis and it occurs in the: 2. Translation is the second step of Protein Synthesis and it occurs in the: 3. If a DNA sequence consists of 12 nucleotides, how many mRNA codons will there be? 4. The enzyme that creates mRNA from a ...
to the PDF file.
to the PDF file.

... •Each DNA codon codes for a separate amino acids •Amino acids have the same basic form including the peptide bond •Variation occurs by having different ‘R’ groups ...
DNA
DNA

DNA - The Double Helix
DNA - The Double Helix

Dna - Quia
Dna - Quia

... 2. Phosphate molecules that alternate with sugar ...
DNA - The Double Helix
DNA - The Double Helix

... The DNA can actually "unzip" when it needs to replicate - or make a copy of itself. DNA needs to copy itself when a cell divides, so that the new cells each contain a copy of the DNA. Without these instructions, the new cells wouldn't have the correct information. The hydrogen bonds are represented ...
DNA.ELECTROPHORESISREVIEW
DNA.ELECTROPHORESISREVIEW

... Define codon and anticodon. Which one determines the amino acid? List differences between DNA and RNA. Explain what an RFLP is how it is used to diagnose sickle cell anemia. Describe how the Rice Krispies simulation is an analogy to protein synthesis. Draw a primary, secondary (alpha and beta) struc ...
nucleic acids - onlinebiosurgery
nucleic acids - onlinebiosurgery

DNA-09 - ChemConnections
DNA-09 - ChemConnections

... labeled with radioactive phosphorus (32P). DNA synthesis takes place, producing a complementary strand of the DNA strand used as a template. ...
chapter outline
chapter outline

... Structural comparison of the genome components of eukaryotes, prokaryotes, and viruses ...
Posted 1/25/07 Mary Case
Posted 1/25/07 Mary Case

... 10-6 conidial/ml. Ten ml. of this suspension is dispensed into a 150X15 mm Petri dish and placed on a magnetic stirrer at 25°C to keep the conidia in suspension. A 15-Watt General Electric germicidal lamp with approximately 90% of its ultraviolet output at 2537 A was used for irradiation. Remove the ...
No Slide Title
No Slide Title

... Bacteria have > 1 protein/mRNA (polycistronic) • Mutations can have polar effects: mutations in upstream genes may affect expression of perfectly good downstream genes! ...
Semester 2 Exam Review
Semester 2 Exam Review

... the complimentary ______________________ (consisting of a _______________,  ...
DNA and RNA Review Sheet Answers
DNA and RNA Review Sheet Answers

... 25. What two other enzymes (that we learned about) are involved in the process of DNA replication Primase – adds RNA primers on both leading and lagging Ligase – glues the backbones of sugars and phosphates together after the RNA primers are removed 26. If the sequence of nucleotides on the original ...
Part Two – Lecture I
Part Two – Lecture I

BCM301 Food Biotechnology
BCM301 Food Biotechnology

... Regulation of mRNA transcription in Eukaryotes (cont.) • A number of diverse, highly specific processes that activate or repress transcription in eukaryotic cells • Generally transcription is mediated by proteins that are collectively classified as transcription factors ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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