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103 Lecture Ch22a
103 Lecture Ch22a

... exactly duplicate the original DNA are produced • The process is called semi-conservative replication because one strand of each daughter DNA comes from the parent DNA and one strand is new • The energy for the synthesis comes from hydrolysis of phosphate groups as the phosphodiester bonds form betw ...
BIOMOLECULES: building blocks of life
BIOMOLECULES: building blocks of life

... – 12 made in the body, – other 8 must consume from nuts, beans, or meat ...
EDVOTEK 225 DNA Fingerprinting
EDVOTEK 225 DNA Fingerprinting

... • Restriction enzymes are endonucleases that catalyze cleavage of phosphate bonds • Require Mg-2 for activity • Generate 5’ phosphae and 3’ hydroxyl group • Endonuclease claves at specific sequence of bases. • Produce by bacteria ...
DNA Analysis in China
DNA Analysis in China

... Genetics Laboratory, Institute of Forensic Sciences People’s Republic of China The Genetics Laboratory of the Institute of Forensic Sciences was the first DNA analysis unit established in China and is China’s central and main DNA profiling laboratory. The laboratory, founded in 1987, began examining ...
Protein Synthesis
Protein Synthesis

Study guide
Study guide

... Favorite music, dyeing hair, choice of clothing style, language spoken • Why do identical twins have the same DNA and not the same personality? Personality is not a result of DNA (inherited traits), but environmental ones instead ...
PowerPoint
PowerPoint

... because comparisons produce a unique pattern that can identify an individual. ...
DNA Oncovirus
DNA Oncovirus

... alone, often do not need metabolic activation, can induce tumor in conjuction with a dose of an initiator that is too low to be carcinogenic alone • Chemicals capable of both initiation and promotion are called complete carcinogens: benzo[a]pyrene and 4aminobiphenyl ...
Sequencing User Guide - ASU Shared Resources
Sequencing User Guide - ASU Shared Resources

New Molecular Based Methods of Diagnosis
New Molecular Based Methods of Diagnosis

... requirements ...
Review of Advanced DNA Structure and Function PPT
Review of Advanced DNA Structure and Function PPT

... parental strand increases the affinity of the enzyme • Absolute requirement for a 3’ end – Incorrect base pairing at the 3’ end is an ineffective substrate – 3’ to 5’ exonuclease activity will remove incorrect base ...
Transcription of DNA into RNA
Transcription of DNA into RNA

... orientation of the mRNA in this subunit shown in the diagram 1 to  the right.  An initiator tRNA, with the anticodoon UAC, base‐pairs  with the start codon, AUG.  This tRNA carries the amino acid  methionine.  The large ribosomal subunit completes the intiation  complex.  In addition to a binding si ...
DNA - Hamilton Local Schools
DNA - Hamilton Local Schools

Chapter 12 Molecular Genetics
Chapter 12 Molecular Genetics

... helicase  Strands kept apart by single-stranded binding proteins  Add “starter” RNA segment by RNA primase  Add new nucleotides by DNA polymerase  This is only the highlights; there are many other enzymes involved ...
Name
Name

... Directions: Open the PowerPoint titled “Translation Tutorial” and press the F5 button to start. Place your keyboard aside (if possible) and only use the mouse. Translation 1. What happens at the ribosome? _________________________________________________________________ 2. Define TRANSLATION. ______ ...
DNA SEQUENCING SAMPLE SUBMISSION FORM
DNA SEQUENCING SAMPLE SUBMISSION FORM

DNA Replication Packet - Mr. Barrow's Science Center
DNA Replication Packet - Mr. Barrow's Science Center

... in nucleotides may occur while making the new DNA strand. ...
eprint_3_17402_800
eprint_3_17402_800

... bacterium Escherichia coli is made up of two strands of DNA that are hydrogen-bonded together to form a single circular molecule comprising 9 million nucleotides. Humans have 46 DNA molecules in each cell, each forming one chromosome. We inherit 23 chromosomes from each parent. Each set of 23 chromo ...
Modern methods in Molecular Pathology
Modern methods in Molecular Pathology

Lesson Overview
Lesson Overview

... replicated, because each base on one strand pairs with only one base on the opposite strand. Each strand of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said t ...
DNA, Mitosis & Meiosis
DNA, Mitosis & Meiosis

... plasma membrane as the cell grows. • Membranous organelles (ER, Golgi, lysosomes, peroxisomes) are made up of the same material as the plasma membrane and must also be made as the cell grows. • Mitochondria have their own DNA and replicate themselves. ...
Chapter 5 DNA and Chromosomes
Chapter 5 DNA and Chromosomes

... Genes – the information-containing elements that determine the characteristics of a species as a whole and of the individuals within it. A gene is usually defined as a segment of DNA that contains the instructions for making a particular protein (or, in some cases, a set of closely related proteins) ...
File
File

... 1. the process by which DNA is copied during the cell cycle 2. nucleus 3. S stage 4. so that every cell will have a complete set of DNA following cell division 5. something that serves as a pattern 6. ATCCATG 7. Proteins help unzip the DNA strand, hold the strands apart, and bond nucleotides togethe ...
Restriction Enzymes
Restriction Enzymes

... that cut DNA at specific sites (typically a four or a 6 base-pair sequence). Bacterial DNA is modified to be protected by methylation while foreign DNA, such as incoming viruses, are not. Usually, organisms that make restriction enzymes also make a companion modification enzyme ( DNA methyltransfera ...
system initial incubation temperature modification study
system initial incubation temperature modification study

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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