Biological Sequences: DNA, RNA, Protein
... • deoxyribonucleic acid (DNA) Basic characteristics of DNA and RNA • DNA has only one biological role, but it is a central one. The information to make all the functional macromolecules of an organism (even DNA itself) is preserved in DNA and accessed through transcription of the information into RN ...
... • deoxyribonucleic acid (DNA) Basic characteristics of DNA and RNA • DNA has only one biological role, but it is a central one. The information to make all the functional macromolecules of an organism (even DNA itself) is preserved in DNA and accessed through transcription of the information into RN ...
DNA mimicry by proteins - Biochemical Society Transactions
... method of control is the use of sequence-specific DNAbinding proteins which block access of another protein, such as RNA polymerase, to the same sequence. The binding affinity of these repressor proteins for their DNA target depends on the cellular environment. Where two such repressor proteins act ...
... method of control is the use of sequence-specific DNAbinding proteins which block access of another protein, such as RNA polymerase, to the same sequence. The binding affinity of these repressor proteins for their DNA target depends on the cellular environment. Where two such repressor proteins act ...
Recombinant DNA Technology
... short peptide called an epitope, for which very specific anitbodies exist. It also gets fused to 6 histidines, which allow easy purification on a column that has nickel ions bound to it (an affinity tag). For growth in mammalian cells, it has an SV40 viral origin of replication (SV40ori), and a zeoc ...
... short peptide called an epitope, for which very specific anitbodies exist. It also gets fused to 6 histidines, which allow easy purification on a column that has nickel ions bound to it (an affinity tag). For growth in mammalian cells, it has an SV40 viral origin of replication (SV40ori), and a zeoc ...
CH_17_6_Genetic_Mutations
... A substitution or point mutation is the replacement of one base in the template strand of DNA with another. If a substitution or point mutation changes the nucleotide, a different amino acid may be inserted into the polypeptide. If this produces no change in the amino acid sequence, it is called a s ...
... A substitution or point mutation is the replacement of one base in the template strand of DNA with another. If a substitution or point mutation changes the nucleotide, a different amino acid may be inserted into the polypeptide. If this produces no change in the amino acid sequence, it is called a s ...
PicoPure DNA Extraction Kit
... Extract DNA for any PCR application DNA extracted with the PicoPure kit is compatible with endpoint PCR performed in the same 0.5 mL tube used for DNA extraction. For gene copy number quantitation, DNA from very small samples can be directly amplified by qPCR without further purification, using plat ...
... Extract DNA for any PCR application DNA extracted with the PicoPure kit is compatible with endpoint PCR performed in the same 0.5 mL tube used for DNA extraction. For gene copy number quantitation, DNA from very small samples can be directly amplified by qPCR without further purification, using plat ...
DNA THIS ONE
... If given a strand of DNA you should be able to: - Identify the other strand of DNA - Determine the mRNA - Determine the amino acids the mRNA will code for Problem: If you are given the following strand of DNA what is the other strand? What will the mRNA look like after transcription of the given str ...
... If given a strand of DNA you should be able to: - Identify the other strand of DNA - Determine the mRNA - Determine the amino acids the mRNA will code for Problem: If you are given the following strand of DNA what is the other strand? What will the mRNA look like after transcription of the given str ...
Principles of BIOCHEMISTRY
... to change their phenotype • Purification of proteins begins with overproduction of the protein in a cell containing the expression vector ...
... to change their phenotype • Purification of proteins begins with overproduction of the protein in a cell containing the expression vector ...
Transcription AND Translation
... • The nucleotides of RNA form hydrogen bonds with the nucleotide bases that are on the strand of DNA. • RNA nucleotides are then linked by RNA polymerase, the transcription enzyme. ...
... • The nucleotides of RNA form hydrogen bonds with the nucleotide bases that are on the strand of DNA. • RNA nucleotides are then linked by RNA polymerase, the transcription enzyme. ...
Chapter 4 Molecular Cloning Methods
... replication has stopped at the tip of the arrowhead. At the end of cycle 1, two DNA duplexes are present, including the region to be amplified, whereas we started with only one. The 5’→3’ polarities of all DNA strands and primers are indicated throughout cycle 1. The same principles apply in cycle 2 ...
... replication has stopped at the tip of the arrowhead. At the end of cycle 1, two DNA duplexes are present, including the region to be amplified, whereas we started with only one. The 5’→3’ polarities of all DNA strands and primers are indicated throughout cycle 1. The same principles apply in cycle 2 ...
Untitled
... Transcription unit has :a promoter, an RNA-coding sequence, and a terminator Promoter: A DNA sequence that the transcription apparatus recognizes and binds. It indicates which of the two DNA strands is to be read as the template. The promoter also determines the transcription start site, the first ...
... Transcription unit has :a promoter, an RNA-coding sequence, and a terminator Promoter: A DNA sequence that the transcription apparatus recognizes and binds. It indicates which of the two DNA strands is to be read as the template. The promoter also determines the transcription start site, the first ...
Communication: Formation of Knots in Partially Replicated DNA
... (Figure 1(b) -(d)) we used the RecA coating method in order to distinguish different types of DNA knots by electron microscopy (Krasnow et al., 1983; Sogo et al., 1987). Figure 2 shows different types of observed knots formed on the isolated replication bubbles (notice the protruding non-replicated ...
... (Figure 1(b) -(d)) we used the RecA coating method in order to distinguish different types of DNA knots by electron microscopy (Krasnow et al., 1983; Sogo et al., 1987). Figure 2 shows different types of observed knots formed on the isolated replication bubbles (notice the protruding non-replicated ...
Chapter 10
... resulting in a hybrid molecule that can be transferred to a host cell, and get replicated in it ...
... resulting in a hybrid molecule that can be transferred to a host cell, and get replicated in it ...
DNA (Deoxyribonucleic acid) is an amazing and fantasizing part of
... Some categories of DNA system damage consist of complexity, mechanism and the fate of damaged DNA (DNA Repair, 1999). There are three major ways in which the cell repairs the DNA. The first system which repairs damaged DNA is the damage reversal systems. They are the simplest; one single enzyme dire ...
... Some categories of DNA system damage consist of complexity, mechanism and the fate of damaged DNA (DNA Repair, 1999). There are three major ways in which the cell repairs the DNA. The first system which repairs damaged DNA is the damage reversal systems. They are the simplest; one single enzyme dire ...
Eukaryotic Transcription
... and small, stable RNAs – RNA polymerase II: synthesis of mRNAs and four small nuclear RNAs that take part in RNA splicing ...
... and small, stable RNAs – RNA polymerase II: synthesis of mRNAs and four small nuclear RNAs that take part in RNA splicing ...
What`s the Point - Biology Junction
... Did this base substitution change the sequence of amino acids --- yes or no? 3. If the base substitution for this same base had been cytosine, not guanine, would the amino acid sequence have been different --- yes or no? Explain why this did or did not produce a change in the amino acid sequence. ...
... Did this base substitution change the sequence of amino acids --- yes or no? 3. If the base substitution for this same base had been cytosine, not guanine, would the amino acid sequence have been different --- yes or no? Explain why this did or did not produce a change in the amino acid sequence. ...
letters The homing endonuclease I-CreI uses three metals
... also important in the LAGLIDADG homing endonuclease ICeuI7. These residues primarily interact with a network of solvent molecules that surround the nucleophilic water molecule (Fig. 4) and extend around the scissile phosphate to the 3′ oxygen leaving group. This network includes a water molecule (nu ...
... also important in the LAGLIDADG homing endonuclease ICeuI7. These residues primarily interact with a network of solvent molecules that surround the nucleophilic water molecule (Fig. 4) and extend around the scissile phosphate to the 3′ oxygen leaving group. This network includes a water molecule (nu ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.