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Practical Applications of DNA Technology
Practical Applications of DNA Technology

... same sequence of four to eight nucleotides is found on both strands, but run in opposite directions  Usually cut the bonds of both strands in a staggered manner, so that he resulting doublestranded DNA fragments have single-stranded ends called sticky ends  The single stranded short extensions for ...
Session 2 – Origin of Life
Session 2 – Origin of Life

... machine can’t get here without the DNA… and the DNA is useless without the machine When it came to the software, he obtained the information from preexisting cells and then modified it and then synthesized DNA of his own (still very impressive, just not creating life in the lab) ...
Messenger RNA
Messenger RNA

... able to produce hundreds or thousands of the same RNA molecules? Proteins must be continuously synthesized in the cell, so the instructions coded in genes must be used over and over again. A single gene must be able to produce hundreds or thousands of the same RNA molecules for protein synthesis. ...
Chapter 13 Chromatin Structure and its Effects on
Chapter 13 Chromatin Structure and its Effects on

... Chromatin Structure and its Effects on Transcription ...
Practice MC Exam - Waterford Union High School
Practice MC Exam - Waterford Union High School

... 25. A DNA Fingerprint is where… a. We examine the patterns on your fingertips to determine what genes you have in your body b. We examine your DNA to determine the patterns created by your fingerprints c. We examine variations in a person’s DNA to determine what aspects are unique to an individual d ...
Chapter 7A
Chapter 7A

... alternative sigma factor, s54, acts very differently in that it binds to its promoters in the absence of the polymerase. s54-RNA polymerase further requires activators that bind to upstream enhancer sequences for initiation. As shown in Fig. 7.4, these "enhancer binding proteins" (e.g., NtrC) contac ...
DNA Damage - Columbus Labs
DNA Damage - Columbus Labs

... code. A decoding, or translation, system exists today as the ribosome and associated factors that are responsible for essentially all polypeptide synthesis from RNA templates in modern organisms. The essence of this mode of polypeptide synthesis is illustrated in Figure 2.8. An RNA molecule (messeng ...
Chapter 16 The Molecular Basis of Inheritance Multiple
Chapter 16 The Molecular Basis of Inheritance Multiple

... 6) In trying to determine whether DNA or protein is the genetic material, Hershey and Chase made use of which of the following facts? A) DNA contains sulfur, whereas protein does not. B) DNA contains phosphorus, but protein does not. C) DNA contains nitrogen, whereas protein does not. D) DNA contain ...
THE GENE: DNA
THE GENE: DNA

... of growth and cell division, the chromosomes, which are made-up of genes, must divide. What features about DNA replication causes each new DNA molecules to be exactly like the original? ...
Chapter 9
Chapter 9

... polytene chromosomes) contains a single giant molecule of DNA. What different levels of organization of this DNA molecule are apparent in chromosomes of eukaryotes at various times during the cell cycle? ANS: DNA during interphase is not yet organized into individual chromosomes, but consists of a s ...
MOLECULAR GENETICS You Are Here* Genes --
MOLECULAR GENETICS You Are Here* Genes --

... Model of Replication is bacterial with DNA polymerase III... several enzymes* form a Replication Complex (Replisome) & include: helicase - untwists DNA topoisomerase [DNA gyrase] - removes supercoils, single strand binding proteins - stabilize replication fork, Primase - makes RNA primer POL III - s ...
02/04
02/04

... Termination RNA polymerase recognizes signals for chain termination. (1) Intrinsic: Termination site on template DNA consists of GC-rich sequences followed by A’s. Intramolecular hydrogen bonding causes formation of hairpin loop. ...
if on the Internet, Press  on your browser to
if on the Internet, Press on your browser to

... A discovery dating back to 1957 (only 4 years after the discovery of the DNA double helix) provides another approach. That year, Gary Felsenfeld, Alexander Rich, and David Davies (all then at the National Institute of Mental Health) created triple helix structures in which a nucleic acid strand atta ...
PART I
PART I

... A molecule of a DNA polymerase binds to one strand of the DNA and begins moving along it in the 3' to 5' direction, using it as a template for assembling a leading strand of nucleotides and reforming a double helix. ...
AP Protein Sythesis
AP Protein Sythesis

... APBiology build RNA 53 ...
DNA RESTRICTION ANALYSIS
DNA RESTRICTION ANALYSIS

... into space marked with red line. There is a leveling bubble which can be used to level the gel casting tray (by turning knobs at bottom). 2. Place tray FLAT where agarose can be poured and allowed to set UNDISTURBED. 3. Carefully pour the entire contents of bottle (40ml of agarose solution) liquifie ...
Chapter 29 DNA as the Genetic Material Recombination of DNA
Chapter 29 DNA as the Genetic Material Recombination of DNA

... • Acridine orange and other aromatic molecules • Intercalation between bases causes added or skipped bases during replication ...
Algorithms for Bioinformatics Autumn 2010
Algorithms for Bioinformatics Autumn 2010

...  It can be argued that penalty of insertion + deletion should be always greater than penalty for one mismatch. ...
Slide 1
Slide 1

... Gel is stained in order to show the location of the DNA fragments and plasmids. ...
Chapter 10 Notes
Chapter 10 Notes

... 19. genetic code- The set of rules that dictates the correspondence between RNA codons in an mRNA molecule and amino acids in protein. 20. RNA polymerase- An enzyme that links together the growing chain of RNA nucleotides during transcription, using a DNA strand as a template. 21. terminator- A spec ...
The Cell Nucleus
The Cell Nucleus

...  deoxyribose - five-carbon sugar  nitrogenous bases • purine – adenine and guanine • pyrimidine – thymine and cytosine ...
chapter 17 from gene to protein
chapter 17 from gene to protein

... Specifically, the polymerase transcribes a DNA sequence called the polyadenylation signal sequence that codes for a polyadenylation sequence (AAUAAA) in the pre-mRNA. ...
DETERMINATION OF NUCLEOTIDE SEQUENCES IN DNA
DETERMINATION OF NUCLEOTIDE SEQUENCES IN DNA

... right for 5 hr with the same polymerisation mixtures. ...
Name: Pd.: ____ Section 11.1 The Work of Gregor Mendel (p. 308
Name: Pd.: ____ Section 11.1 The Work of Gregor Mendel (p. 308

... 5. If T represents the allele for tall and t represents the allele for short and you cross a TT plant with a Tt plant: a. Which parent is homozygous dominant? _________________________________ b. Which parent is heterozygous? __________________________________________ c. What is (are) the genotype(s ...
Protein Synthesis - Austin Community College
Protein Synthesis - Austin Community College

... Specifically, the polymerase transcribes a DNA sequence called the polyadenylation signal sequence that codes for a polyadenylation sequence (AAUAAA) in the pre-mRNA. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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