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Protein Synthesis - Austin Community College
Protein Synthesis - Austin Community College

... Specifically, the polymerase transcribes a DNA sequence called the polyadenylation signal sequence that codes for a polyadenylation sequence (AAUAAA) in the pre-mRNA. ...
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DETERMINATION OF NUCLEOTIDE SEQUENCES IN DNA

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chapter 12 - Fullfrontalanatomy.com
chapter 12 - Fullfrontalanatomy.com

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Chapter 27
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... 4. Triploid: having 1 extra of every homologous pair (69) chromosomes) 5. Polyploidy- sometimes all 22 chromosomal pairs fail to separate. The resulting 2n gamete fuses with the normal n gamete, producing a 3n zygote. This is common in plants but rare in humans ...
MLPA Assay using GSP Kit
MLPA Assay using GSP Kit

... By analysing the fluorescence levels for the gene of interest with reference to a series of control peaks and a reference DNA sample, it is possible to accurately quantify the copy number of the gene/exon of interest. This therefore detects events such as duplications and deletions in individual gen ...
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DNA Recombination
DNA Recombination

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Chapter10_Outline
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... • Typically, a genomic sequence contains many gaps that prevent the contigs from being assembled. • BAC clones are important because the sequences at the extreme ends of the cloned fragments give long-range information that allows adjacent contigs to be recognized and assembled in the correct orient ...
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... (osmotic pressure), temperature, and pH. Which of these terms pertain most to microorganisms found on or in human beings? What do obligate and facultative mean? Know specific terms most likely to describe the environmental preferences of bacteria prevalent in human disease. Know the bacterial growth ...
Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... – mating pair formation (Mpf) component: proteins involved in forming transfer structure • Membrane associated • Sex pilus ...
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ANSWER - EdWeb

... a. Point mutation ANSWER: a. Point mutations mean one base is substituted for the wrong base (Ex: Adenine is accidentally put where Guanine should be) – it may or may not affect the organism. ...
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... • Experiments with bacteria and viruses in the 1940s began to implicate DNA as the genetic material • Beadle and Tatum formulated the one gene-one enzyme concept (each gene is responsible for the production of a single protein) • 1953 - Watson and Crick, with assistance from Rosalind Franklin, propo ...
Chapter 8: From DNA to Proteins
Chapter 8: From DNA to Proteins

... Proteins carry out the process of replication.  DNA only stores information, enzymes and other proteins do the actual work of replication.  DNA polymerase helps bond nucleotides together during replication. The Replication Process 1. Enzymes unzip the double helix at various points. 2. Bases on ea ...
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Gene expression (central dogma)

... During translation, the nucleotide sequence of an mRNA is translated into the amino acid sequence of a polypeptide. Specifically, the nucleotides of the mRNA are read in triplets (groups of three) called codons. There are 616161codons that specify amino acids. One codon is a "start" codon that indic ...
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Chapter 17 Gene To Protein

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... 48. Crossing over is such a potent source of genetic variation because it always leads to the gain or loss of chromatin. a. T b. F 49. The X and Y sex chromosomes typically show a. Higher levels of recombination than autosomes b. The same levels of recombination as autosomes c. Less recombination th ...
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Plant RNA/DNA Purification Kit

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Structure and Analysis of DNA - Circle

... The bases in DNA will only pair in very specific ways, G with C and A with T In short DNA sequences, imprecise base pairing will not be tolerated Long sequences can tolerate some mispairing only if -G of the majority of bases in a sequence exceeds the energy required to keep mispaired bases togethe ...
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... When a virus insert its DNA into the bacteria, the transcribed ‘spacers’, which are about 60 nucleotides long RNA molecules complimentary to the piece of viral DNA, will bind to the invading viral DNA and by using the endonuclease‐like Cas9 (CRISPR associated protein 9) it can make double strand bre ...
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Nucleotides and nucleic acids - Delivery guide

... • Once the nucleotides are made, join these to form a single-stranded polynucleotide. (The strand should contain at least 10 nucleotides which can be joined in random sequence to provide some interest in later activities involving translation and transcription). • Using this strand as a template ...
aneuploidy
aneuploidy

... sequence of DNA nucleotide bases ...
< 1 ... 173 174 175 176 177 178 179 180 181 ... 657 >

Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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