Recombinant DNA - Rose
... which are relatively short regions containing large numbers of unique restriction sites. A well-designed example of a cloning vector is pUC18, which is distantly related to pBR322, but is somewhat smaller. Vectors such as pUC18 are high copy number plasmids, because they contain highly efficient ori ...
... which are relatively short regions containing large numbers of unique restriction sites. A well-designed example of a cloning vector is pUC18, which is distantly related to pBR322, but is somewhat smaller. Vectors such as pUC18 are high copy number plasmids, because they contain highly efficient ori ...
Select one of your Biology instructors from another class and look
... 3.1 Emmer wheat (Triticum dicoccum) has a somatic chromosome number of 28, and rye (Secale cereale) has a somatic chromosome number of 14. Hybrids produced by crossing these cereal grasses are highly sterile and have many characteristics intermediate between the parental species. How many chromosome ...
... 3.1 Emmer wheat (Triticum dicoccum) has a somatic chromosome number of 28, and rye (Secale cereale) has a somatic chromosome number of 14. Hybrids produced by crossing these cereal grasses are highly sterile and have many characteristics intermediate between the parental species. How many chromosome ...
DNA Extraction Lab
... enough alcohol so that you have equal parts of both liquids. 10. Dip the bamboo skewer into the test tube where the alcohol and strawberry layers meet. Pull up the skewer. The whitish, stringy stuff is DNA containing strawberry genes! ...
... enough alcohol so that you have equal parts of both liquids. 10. Dip the bamboo skewer into the test tube where the alcohol and strawberry layers meet. Pull up the skewer. The whitish, stringy stuff is DNA containing strawberry genes! ...
Shotgun sequencing
... special methods are required. An obvious solution is to sequence as far as you can from your primer; then synthesize a new primer near the end of the known sequence; and repeat. Works, but at best you’d be able to sequence maybe 500 bases a day—making it impossible to sequence something like the hum ...
... special methods are required. An obvious solution is to sequence as far as you can from your primer; then synthesize a new primer near the end of the known sequence; and repeat. Works, but at best you’d be able to sequence maybe 500 bases a day—making it impossible to sequence something like the hum ...
Bacteria - REMC 8 / Kent ISD Moodle VLE
... •If the F factor is found on the bacterial chromosome, the cell containing the F factor is called an Hfr cell. (Hfr = High frequency of recombination) •An Hfr cell serves as a donor during conjugation, and the recipient cell is still called an F- cell. •When DNA from the Hfr cell enters, it i ...
... •If the F factor is found on the bacterial chromosome, the cell containing the F factor is called an Hfr cell. (Hfr = High frequency of recombination) •An Hfr cell serves as a donor during conjugation, and the recipient cell is still called an F- cell. •When DNA from the Hfr cell enters, it i ...
Chapter 17.
... determined mRNA–amino acid match added fabricated mRNA to test tube of ribosomes, tRNA & amino acids ...
... determined mRNA–amino acid match added fabricated mRNA to test tube of ribosomes, tRNA & amino acids ...
Structure of the DNA-binding motifs of activators
... – General TF cause a stepwise build-up of preinitiation complex – General TF and other proteins are already bound to polymerase in a complex called RNA polymerase holoenzyme ...
... – General TF cause a stepwise build-up of preinitiation complex – General TF and other proteins are already bound to polymerase in a complex called RNA polymerase holoenzyme ...
Notes Protein Synthesis 2016
... • The order of the nitrogen bases tell your cells to make certain proteins. • There are ~ 3 billion bases in the DNA code in each cell of your body. Each cell has enough bases to fill 200 pages of this book in small font. ...
... • The order of the nitrogen bases tell your cells to make certain proteins. • There are ~ 3 billion bases in the DNA code in each cell of your body. Each cell has enough bases to fill 200 pages of this book in small font. ...
DNA to Protein
... • A tRNA molecule has 75 to 80 nucleotides and a threedimensional shape (conformation). • The shape is maintained by complementary base pairing and hydrogen bonding. • The three-dimensional shape of the tRNAs allows them to combine with the binding sites of the ribosome. ...
... • A tRNA molecule has 75 to 80 nucleotides and a threedimensional shape (conformation). • The shape is maintained by complementary base pairing and hydrogen bonding. • The three-dimensional shape of the tRNAs allows them to combine with the binding sites of the ribosome. ...
Editable PPT - Science Prof Online
... Q: What base does RNA have that DNA doesn’t have? Q: What base does DNA ...
... Q: What base does RNA have that DNA doesn’t have? Q: What base does DNA ...
Robust PCR amplification of GC-rich targets with Hot Start 7
... amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of the DNA polymerase to negotiate through regions of strong secondary structure, such as hairpins, often results in the formation of ...
... amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of the DNA polymerase to negotiate through regions of strong secondary structure, such as hairpins, often results in the formation of ...
Diapositive 1
... PPP generates NADPH, involved in the majority of anabolic pathways: 1 mole of Glucose 6 phosphate 2 moles of NADPH NADPH allows methionine to be recycled from homocysteine, ( methylene tetrahydrofolate reductase) This pathway influences imprinting process and is involved in thymidine synthesis (5 Me ...
... PPP generates NADPH, involved in the majority of anabolic pathways: 1 mole of Glucose 6 phosphate 2 moles of NADPH NADPH allows methionine to be recycled from homocysteine, ( methylene tetrahydrofolate reductase) This pathway influences imprinting process and is involved in thymidine synthesis (5 Me ...
Aalborg Universitet profiling of anaerobic digesters
... Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. ? Users may download a ...
... Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. ? Users may download a ...
DNA Purity Instruments
... Traditionally, UV lamps such as xenon flash or mercury have been used as light sources for these measurements since they have a broad spectrum and sufficient intensity in the desired wavelengths. ...
... Traditionally, UV lamps such as xenon flash or mercury have been used as light sources for these measurements since they have a broad spectrum and sufficient intensity in the desired wavelengths. ...
"Polymerase Chain Reaction (PCR)". In: Encyclopedia of Life Sciences
... This technique involves using RNA rather than DNA as the template for amplification. The procedure is very similar to conventional PCR but includes an initial step in which a DNA copy of the RNA template is produced using the enzyme reverse transcriptase. This enzyme, which is of viral origin, is a ...
... This technique involves using RNA rather than DNA as the template for amplification. The procedure is very similar to conventional PCR but includes an initial step in which a DNA copy of the RNA template is produced using the enzyme reverse transcriptase. This enzyme, which is of viral origin, is a ...
Modeling Meiosis with Pop Beads
... Assemble two strands of yellow beads connected to magnetic centromeres and two strands of red beads connected to magnetic centromeres. One of the red strands represents the chromosome contribution of the female parent, and one of the yellow strands represents the chromosome contribution of the male ...
... Assemble two strands of yellow beads connected to magnetic centromeres and two strands of red beads connected to magnetic centromeres. One of the red strands represents the chromosome contribution of the female parent, and one of the yellow strands represents the chromosome contribution of the male ...
9.1 Manipulating DNA - SBI4u Biology Resources
... • You have a vial of undescribed DNA fragments—now what? • Samples pipetted into wells on one end of a gel (e.g., agarose) • Electricity is added to the gel • DNA fragments move through the gel at different rates, away from the negative and toward the positive end – Smaller fragments move easier and ...
... • You have a vial of undescribed DNA fragments—now what? • Samples pipetted into wells on one end of a gel (e.g., agarose) • Electricity is added to the gel • DNA fragments move through the gel at different rates, away from the negative and toward the positive end – Smaller fragments move easier and ...
Biotechnology, Part I
... In our example, the human gene for insulin production is combined with a plasmid. ...
... In our example, the human gene for insulin production is combined with a plasmid. ...
Genomic DNA & cDNA Libraries
... walking, it is possible to order to genomic clones. As I mentioned earlier the human genome contains 3 × 10E9 base pairs. Each vector contains 20kb that means that it is necessary to generate about 1.5 x 10E5 phages. You can screen 5 × 10E4 plaques on each petri dish meaning that you can contain all ...
... walking, it is possible to order to genomic clones. As I mentioned earlier the human genome contains 3 × 10E9 base pairs. Each vector contains 20kb that means that it is necessary to generate about 1.5 x 10E5 phages. You can screen 5 × 10E4 plaques on each petri dish meaning that you can contain all ...
fall break, take home exam
... purification of nucleic acids. Use all of the following words: silica, DEAE, positive charge, pH, salt concentration, negative charge, bind, rinse, elute (9 points). ...
... purification of nucleic acids. Use all of the following words: silica, DEAE, positive charge, pH, salt concentration, negative charge, bind, rinse, elute (9 points). ...
Chapter 12
... § Plasmid DNA is isolated § DNA containing the gene of interest is isolated § Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle § DNA with the target gene is treated with the same enzyme and many fragments are produced § Plasmid and target DNA are mixed and as ...
... § Plasmid DNA is isolated § DNA containing the gene of interest is isolated § Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle § DNA with the target gene is treated with the same enzyme and many fragments are produced § Plasmid and target DNA are mixed and as ...
Thermophiles_12May06..
... The temperatures at which thermophiles grow optimally start at 45 oC (113oF) and extend well beyond that. Scientists have known about thermophiles for over 40 years. It has been only recently that microbiologists have discovered that the temperatures at which some life can thrive far exceeds 45 oC. ...
... The temperatures at which thermophiles grow optimally start at 45 oC (113oF) and extend well beyond that. Scientists have known about thermophiles for over 40 years. It has been only recently that microbiologists have discovered that the temperatures at which some life can thrive far exceeds 45 oC. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.