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PDF - Available Technologies
PDF - Available Technologies

... Production of recombinant protein or gene products in bacteria or other cells is dramatically increased when the TEnBOX is incorporated into the plasmid vector. The efficiency of the protein expression from the TEnBOX is much higher than reported with the best commercial vectors (pET-21 and pTriEX-3 ...
The cost of life is energy.
The cost of life is energy.

... price” of living by CATALYZING (or helping) reactions they need to stay alive. These reactions are called the METABOLISM. • Enzymes work to SYNTHESIZE molecules and break them apart. ...
Analytical Sciences, Poster AS-101 Kinetics and identification of non
Analytical Sciences, Poster AS-101 Kinetics and identification of non

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Biological Building Blocks Andrew Rylaarsdam

... After synthesizing and purifying these peptides, we were able to characterize them. First we verified that they were of the correct size and sequence, and second that they folded as we had hoped. With these peptides synthesized and characterized, we are beginning to study how they bind to metals, an ...
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From gene to protein 2

... To assemble correctly with other proteins To bind with small-molecule cofactors that are required for their activity To be appropriately modified by protein kinases or other proteinmodifying enzymes ...
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Competition
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...  small units called amino acids bonded together There are only 20 types of amino acids, but there are millions of proteins. Therefore the number of amino acids, and their sequence determine the protein. Only 12 of the amino acids can be made by our body, the rest have to come from our food (meat, n ...
BioCore II lecture6
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... Here, the caramel takes on a tapioca-like structure, with large visible aggregates of protein structures (Figure 1), as it loses its desirable smooth texture. There are two general categories of proteins in milk — the caseins (≈80%) and the serum proteins (≈20%). The various casein proteins form int ...
Proceedings of a meeting held at Allerton House, Monticello, Illinois
Proceedings of a meeting held at Allerton House, Monticello, Illinois

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Macromolecules of life: Structure-function and Bioinformatics 356
Macromolecules of life: Structure-function and Bioinformatics 356

... Perspectives on the flow of information from nucleic acids to proteins, the structure and functions of nucleic acids and proteins and their organisation into hierarchical, interdependent systems. Nucleic acid structure as observed in fibres and crystals as well as global DNA and RNA analyses (method ...
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Biochemistry H Silent Tea Party Name_______________ 1. What is

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Proteins & Nucleic Acids - St. Mary Catholic Secondary School
Proteins & Nucleic Acids - St. Mary Catholic Secondary School

... Tertiary shape is held together by R-group bonding within the chain and R-group interactions with the environment. Tertiary structure is also aided by prosthetic groups that are inorganic compounds that act as a central point for bonding within the protein. Quaternary structure occurs when a few ter ...
Characterization of head-hunter proteins for exchange of genetic information between cells.
Characterization of head-hunter proteins for exchange of genetic information between cells.

... information is highly conserved among bacteria. During evolution, the machinery has been modified to export other substrates, like DNA and proteins into a wide variety of organisms, including bacterial and eukaryotic cells. Agrobacterium tumefaciens VirB/D4 secretion system is an excellent model to ...
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Q24 Compare and contrast peptide and steroid hormones. Give four

... Range  from  small  peptides  (3-­‐200  amino  acids).   Highly  lipid  soluble;  consist  of  three  cyclohexal   Generally  referred  to  as  peptides  if  <100  amino   ring  and  one  cyclopental  ring  in  a  single   acids,  and   ...
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Protein Structure

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Intro to Biochem - Winston Knoll Collegiate
Intro to Biochem - Winston Knoll Collegiate

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L2_Principle of protein folding in the cellular environment

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Chapter 20 Amino acids and proteins

... 2. Briefly, summarize the process of electrophoresis. 3. Given three or four amino acids, their pIs, and the pH of the buffer, determine the movement of the amino acids on an electrophoresis gel. 20.4 formation of peptides 1. Draw the structure of a dipetide from the zwitterions of two or more amino ...
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Cyclol



The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.
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