Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... poliovirus 2A protein which occurs while the polypeptide is still nascent on the ribosome. The peptide bond hydrolysed in this initial processing step is between a tyrosine and a glycine residue which connect the P1 and P2 regions (Nicklin et al., 1987; Toyoda et al., 1986a, b) (Fig. 1) whereas most ...
... poliovirus 2A protein which occurs while the polypeptide is still nascent on the ribosome. The peptide bond hydrolysed in this initial processing step is between a tyrosine and a glycine residue which connect the P1 and P2 regions (Nicklin et al., 1987; Toyoda et al., 1986a, b) (Fig. 1) whereas most ...
A chitin-binding domain in a marine bacterial
... only one cysteine and is not similar to that of plants (Watanabe et al., 1994). Recently, Morimoto et al. (1997)found several other similar residues among chitinbinding domains from bacterial chitinases and observed that removal of the binding domain decreased hydrolysis of unprocessed chitin by a c ...
... only one cysteine and is not similar to that of plants (Watanabe et al., 1994). Recently, Morimoto et al. (1997)found several other similar residues among chitinbinding domains from bacterial chitinases and observed that removal of the binding domain decreased hydrolysis of unprocessed chitin by a c ...
Malonyl-CoA and AMP-activated protein kinase: An expanding
... utilization as well as synthesis may be regulated. Since muscle does not use malonyl CoA for fatty acid synthesis, as does liver, a principal focus has been on malonyl CoA decarboxylase (MCD). Evidence has been presented that MCD is present in both cardiac [36–40] and skeletal muscle [8, 41–43]. In ...
... utilization as well as synthesis may be regulated. Since muscle does not use malonyl CoA for fatty acid synthesis, as does liver, a principal focus has been on malonyl CoA decarboxylase (MCD). Evidence has been presented that MCD is present in both cardiac [36–40] and skeletal muscle [8, 41–43]. In ...
reprint - Charles Brenner Laboratory
... coA for the a-ketoglutarate dehydrogenase (a-KGDH) complex to form succinyl-coA. In addition, there must be GDP or ADP for the succinyl-coA synthetase reaction to form succinate, and there must be a supply of each citric acid cycle intermediate to keep the cycle running. Fuel oxidation is coupled to ...
... coA for the a-ketoglutarate dehydrogenase (a-KGDH) complex to form succinyl-coA. In addition, there must be GDP or ADP for the succinyl-coA synthetase reaction to form succinate, and there must be a supply of each citric acid cycle intermediate to keep the cycle running. Fuel oxidation is coupled to ...
Ketone Body Metabolism
... zAfter the diet has been changed to lower blood glucose for 3 days, the brain gets 30% of its energy from ketone bodies. zAfter 40 days, this goes up to 70% (during the initial stages the brain does not burn ketones, since they are an important substrate for lipid synthesis in the brain). zThe brain ...
... zAfter the diet has been changed to lower blood glucose for 3 days, the brain gets 30% of its energy from ketone bodies. zAfter 40 days, this goes up to 70% (during the initial stages the brain does not burn ketones, since they are an important substrate for lipid synthesis in the brain). zThe brain ...
The introduction and use of PKU sphere™, a Glycomacropeptide
... In the 1950’s, the first protein substitute (PS), which was based on protein hydrolysate, was developed to treat the first patient with PKU managed with diet therapy1. For the last 30 years, the most commonly used PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originall ...
... In the 1950’s, the first protein substitute (PS), which was based on protein hydrolysate, was developed to treat the first patient with PKU managed with diet therapy1. For the last 30 years, the most commonly used PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originall ...
Metabolism of bile acids
... into hepatocytes, thereby completing the enterohepatic cycle. Bile acids serve a number of functions: (i) they are the main solutes in bile and, as such, they are important for the generation of the so-called bile salt-dependent bile flow; (ii) bile salts are indispensable for the secretion of chole ...
... into hepatocytes, thereby completing the enterohepatic cycle. Bile acids serve a number of functions: (i) they are the main solutes in bile and, as such, they are important for the generation of the so-called bile salt-dependent bile flow; (ii) bile salts are indispensable for the secretion of chole ...
Glycolic acid production in the engineered yeasts Saccharomyces
... compounds such as ethanol are naturally converted into glyoxylate, which can further be converted into glycolic acid by metabolic engineering. Although the main function of the glyoxylate cycle is to ensure the availability of four carbon compounds by utilising two carbon substrates, the naturally e ...
... compounds such as ethanol are naturally converted into glyoxylate, which can further be converted into glycolic acid by metabolic engineering. Although the main function of the glyoxylate cycle is to ensure the availability of four carbon compounds by utilising two carbon substrates, the naturally e ...
Note - EtoosIndia
... ATPase activity is found in F1 (head) which is protruded towards matrix of the mitochondria. F0 (base) which is embedded in inner membrane has proton channels. ATPase becomes active only when proton gradient develops. Passage of 2e– from NADH2 pushes out three pairs of protons to outer chamber ...
... ATPase activity is found in F1 (head) which is protruded towards matrix of the mitochondria. F0 (base) which is embedded in inner membrane has proton channels. ATPase becomes active only when proton gradient develops. Passage of 2e– from NADH2 pushes out three pairs of protons to outer chamber ...
Peer-reviewed Article PDF
... of 437 amino acid residues are organized into two main subunits, each consisting of 20 alpha-helices. These alpha helices compose approximately 75% of citrate synthase’s tertiary structure. Between these two subunits, a single cleft exists containing the active site. Two binding sites can be found t ...
... of 437 amino acid residues are organized into two main subunits, each consisting of 20 alpha-helices. These alpha helices compose approximately 75% of citrate synthase’s tertiary structure. Between these two subunits, a single cleft exists containing the active site. Two binding sites can be found t ...
32_Metabolism of ammonia. Biosynthesis of urea and its disorders
... Deficiency of any of the five enzymes in the urea cycle results in the accumulation of ammonia and leads to encephalopathy. Episodes of encephalopathy and associated systems are unpredictable and, if untreated, are lethal or produce devastating neurologic sequelae in long-term survivors. Although th ...
... Deficiency of any of the five enzymes in the urea cycle results in the accumulation of ammonia and leads to encephalopathy. Episodes of encephalopathy and associated systems are unpredictable and, if untreated, are lethal or produce devastating neurologic sequelae in long-term survivors. Although th ...
Molecular Characterisation of the 76 kDa Iron
... Universitdt Ulm, Allgemeine Botanik, Albert-Einstein-Allee, D-89069 Vim, Germany Max-Volmer-Institut fur Biophysikalische Chemie und Biochemie, Technische Universitdt Berlin, Strasse des 17. Juni 135, D-10623 Berlin, Germany ...
... Universitdt Ulm, Allgemeine Botanik, Albert-Einstein-Allee, D-89069 Vim, Germany Max-Volmer-Institut fur Biophysikalische Chemie und Biochemie, Technische Universitdt Berlin, Strasse des 17. Juni 135, D-10623 Berlin, Germany ...
Chapter 5 Endocrine Regulation of Glucose - Rose
... involved are easily reversible, and are common to both pathways. However, the reactions catalyzed by phosphofructokinase and pyruvate kinase are not reversible. The gluconeogenic pathway must use different enzymes to reverse these steps. These enzymes (phosphoenolpyruvate carboxykinase and fructose- ...
... involved are easily reversible, and are common to both pathways. However, the reactions catalyzed by phosphofructokinase and pyruvate kinase are not reversible. The gluconeogenic pathway must use different enzymes to reverse these steps. These enzymes (phosphoenolpyruvate carboxykinase and fructose- ...
Isolation of Rat Transferrin Using CNBr-Activated
... Immuno adsorbent columns, loaded with transferrin or antitransferrin, were used over a period of more than two years without loss of activity. ...
... Immuno adsorbent columns, loaded with transferrin or antitransferrin, were used over a period of more than two years without loss of activity. ...
The activity and kinetic properties of cellulases in substrates
... The first two enzymes in this category act directly on cellulose, yielding mainly cellobiose and glucose as reaction products, and then the cellobiose is hydrolyzed into glucose by cellobiase [3,6]. Although the crude cellulase has a lower specific activity than pure cellulase containing only one of ...
... The first two enzymes in this category act directly on cellulose, yielding mainly cellobiose and glucose as reaction products, and then the cellobiose is hydrolyzed into glucose by cellobiase [3,6]. Although the crude cellulase has a lower specific activity than pure cellulase containing only one of ...
Eram_SeyedMohammad - UWSpace
... respectively, while the T. maritima enzyme had a specific activity of 90.8±11 and 1.4±0.15 Umg-1 for POR and PDC, respectively. A novel enzyme capable of catalyzing the production of acetaldehyde from pyruvate in hyperthermophiles was also characterized. This enzyme is a TPP- and flavin-containing a ...
... respectively, while the T. maritima enzyme had a specific activity of 90.8±11 and 1.4±0.15 Umg-1 for POR and PDC, respectively. A novel enzyme capable of catalyzing the production of acetaldehyde from pyruvate in hyperthermophiles was also characterized. This enzyme is a TPP- and flavin-containing a ...
2. Citric acid cycle
... III. Steps of Respiration IV. Cellular Respiration A. Glycolysis B. Coenzyme Junction C. Citric Acid Cycle (aka Krebs/TCA cycle) D. Electron Transport Chain (ETC) E. Chemiosmosis ...
... III. Steps of Respiration IV. Cellular Respiration A. Glycolysis B. Coenzyme Junction C. Citric Acid Cycle (aka Krebs/TCA cycle) D. Electron Transport Chain (ETC) E. Chemiosmosis ...
Natural Carbon Isotope Abundance of Plasma
... (mainly from ingestion of C3 and C4 metabolism plants) and to the discrimination between ‘light’ and ‘heavy’ isotopes during biochemical reactions (isotope effects and isotopic fractionation). Methodology/Principal Findings: Carbon isotopic abundance was measured in ZDF (fa/+) and ZDF (fa/fa), (lean ...
... (mainly from ingestion of C3 and C4 metabolism plants) and to the discrimination between ‘light’ and ‘heavy’ isotopes during biochemical reactions (isotope effects and isotopic fractionation). Methodology/Principal Findings: Carbon isotopic abundance was measured in ZDF (fa/+) and ZDF (fa/fa), (lean ...
Calculation of Biochemical Net Reactions and Pathways by Using
... The calculation of a biochemical pathway is based on the stoichiometric number matrix for the biochemical reactions that can occur in the system. A stoichiometric number matrix has a column for each reaction and a row for each reactant. Reactants on the left have stoichiomctric numbers with negative ...
... The calculation of a biochemical pathway is based on the stoichiometric number matrix for the biochemical reactions that can occur in the system. A stoichiometric number matrix has a column for each reaction and a row for each reactant. Reactants on the left have stoichiomctric numbers with negative ...
Fat to the fire: the regulation of lipid oxidation
... area of active investigation. Indeed, the integration between cellular regulation of lipid metabolism and whole-body exercise performance is a fascinating but often overlooked research area. Additionally, the interaction between environmental stress, exercise, and lipid oxidation has not been suffic ...
... area of active investigation. Indeed, the integration between cellular regulation of lipid metabolism and whole-body exercise performance is a fascinating but often overlooked research area. Additionally, the interaction between environmental stress, exercise, and lipid oxidation has not been suffic ...
Src protein–tyrosine kinase structure and regulation
... R. Roskoski Jr. / Biochemical and Biophysical Research Communications 324 (2004) 1155–1164 ...
... R. Roskoski Jr. / Biochemical and Biophysical Research Communications 324 (2004) 1155–1164 ...
The Structure of the PanD/PanZ Protein Complex
... key enzymes (Farrar et al., 2010), or metabolite-binding riboswitches (Winkler and Breaker, 2005). The pathway from pantothenate onward to CoA is regulated by feedback inhibition of pantothenate kinase (Rock et al., 2003; Yun et al., 2000) in all organisms, including bacteria, but no evidence for re ...
... key enzymes (Farrar et al., 2010), or metabolite-binding riboswitches (Winkler and Breaker, 2005). The pathway from pantothenate onward to CoA is regulated by feedback inhibition of pantothenate kinase (Rock et al., 2003; Yun et al., 2000) in all organisms, including bacteria, but no evidence for re ...
Evaluation of Corn Distillers Grains and Ruminally Protected Lysine
... meal, 2 ) soybean meal plus ruminally protected Lys and Met, 3 ) corn distillers grains, and 4 ) corn distillers grains plus ruminally protected Lys and Met. Dry matter intakes were lower for cows fed diets containing soybean meal than for cows fed diets containing corn distillers grains. Milk yield ...
... meal, 2 ) soybean meal plus ruminally protected Lys and Met, 3 ) corn distillers grains, and 4 ) corn distillers grains plus ruminally protected Lys and Met. Dry matter intakes were lower for cows fed diets containing soybean meal than for cows fed diets containing corn distillers grains. Milk yield ...