Malonate decarboxylase of Pseudomonas putida is composed of
... duced amino acid sequence of the gene encoding malonate decarboxylase from K. pneumoniae. The Klebsiella enzyme, however, was composed of the four subunits K (MdcA), L (MdcD), Q (MdcE), and N (MdcC) lacking MdcH [6]. There are several discrepancies between Pseudomonas enzyme and Klebsiella enzyme in ...
... duced amino acid sequence of the gene encoding malonate decarboxylase from K. pneumoniae. The Klebsiella enzyme, however, was composed of the four subunits K (MdcA), L (MdcD), Q (MdcE), and N (MdcC) lacking MdcH [6]. There are several discrepancies between Pseudomonas enzyme and Klebsiella enzyme in ...
Hydrophobins from Aspergillus species cannot be clearly divided
... Several groups are revealed in the phylogenetic tree (Additional file 1) and it seems that hydrophobins cluster according to their cysteine spacing pattern. A common feature in 44 of the 50 hydrophobins is a conserved spacing of five amino acids between the fifth and sixth cysteines, while the remai ...
... Several groups are revealed in the phylogenetic tree (Additional file 1) and it seems that hydrophobins cluster according to their cysteine spacing pattern. A common feature in 44 of the 50 hydrophobins is a conserved spacing of five amino acids between the fifth and sixth cysteines, while the remai ...
Chapter 27 Slides
... First step: ribose-5-P must be activated - by PPi PRPP is limiting substance for purine synthesis But PRPP is a branch point so next step is the committed step - Gln PRPP amidotransferase Note that second step changes C-1 configuration G- and A-nucleotides inhibit this step - but at distinct sites! ...
... First step: ribose-5-P must be activated - by PPi PRPP is limiting substance for purine synthesis But PRPP is a branch point so next step is the committed step - Gln PRPP amidotransferase Note that second step changes C-1 configuration G- and A-nucleotides inhibit this step - but at distinct sites! ...
The Metabolism of Acetate by the Blue-green Algae
... in the presence of sodium acetate and absence of carbon dioxide were unsuccessful. The failure of acetate and other substrates to stimulate respiration in blue-green algae has been known for some time (Kratz & Myers, 1955b)and in our experiments A . variabilis was no exception. Over a range of p H v ...
... in the presence of sodium acetate and absence of carbon dioxide were unsuccessful. The failure of acetate and other substrates to stimulate respiration in blue-green algae has been known for some time (Kratz & Myers, 1955b)and in our experiments A . variabilis was no exception. Over a range of p H v ...
Chapter 10: Anorexia Nervosa and Bulimia Nervosa
... Organic compound found in foods, noncaloric Required in small amounts Required in the diet (essential) Proven to be required for health, growth, and reproduction ; deficiency syndrome identified There are 4 fat soluble vitamins & 9 water soluble vitamins for a total of 13. Your text also lists choli ...
... Organic compound found in foods, noncaloric Required in small amounts Required in the diet (essential) Proven to be required for health, growth, and reproduction ; deficiency syndrome identified There are 4 fat soluble vitamins & 9 water soluble vitamins for a total of 13. Your text also lists choli ...
A Survey of Left-handed Helices in Protein Structures
... University, Box 596, SE-751 24 Uppsala, Sweden ...
... University, Box 596, SE-751 24 Uppsala, Sweden ...
Inducible uptake and metabolism of glucose by the phosphorylative
... and then subsequently to 6-phosphogluconate by extracellular, high affinity, glucose dehydrogenase and gluconate dehydrogenase (Quay et al., 1972; Midgley & Dawes, 1973; Roberts et al., 1973; Lessie et al., 1979), and (ii) the intracellular, low affinity, nucleotide-dependent phosphorylative pathway ...
... and then subsequently to 6-phosphogluconate by extracellular, high affinity, glucose dehydrogenase and gluconate dehydrogenase (Quay et al., 1972; Midgley & Dawes, 1973; Roberts et al., 1973; Lessie et al., 1979), and (ii) the intracellular, low affinity, nucleotide-dependent phosphorylative pathway ...
An Introduction to Enzyme Science
... substance results in a metabolically significant conformational change. In most cases, modulating effects are negative (i.e., they result in inhibition), but positive effects (i.e., those resulting in activation) are also known. Feedback regulation has proven to be a highly effective strategy for co ...
... substance results in a metabolically significant conformational change. In most cases, modulating effects are negative (i.e., they result in inhibition), but positive effects (i.e., those resulting in activation) are also known. Feedback regulation has proven to be a highly effective strategy for co ...
Chapter 9 Cellular Respiration: Harvesting Chemical
... 30) Why is coenzyme A, a sulfur containing molecule derived from a B vitamin, added? A) because sulfur is needed for the molecule to enter the mitochondrion B) in order to utilize this portion of a B vitamin which would otherwise be a waste product from another pathway C) to provide a relatively uns ...
... 30) Why is coenzyme A, a sulfur containing molecule derived from a B vitamin, added? A) because sulfur is needed for the molecule to enter the mitochondrion B) in order to utilize this portion of a B vitamin which would otherwise be a waste product from another pathway C) to provide a relatively uns ...
Metabolic significance of inorganic triphosphate, thiamine
... this superfamily have been characterized and they all have in common that they act on triphosphorylated substrates. Some bacterial members (the N. europeae specifically) hydrolyze inorganic triphosphate (PPPi) which raises the question of the physiological significance of this compound. We first stu ...
... this superfamily have been characterized and they all have in common that they act on triphosphorylated substrates. Some bacterial members (the N. europeae specifically) hydrolyze inorganic triphosphate (PPPi) which raises the question of the physiological significance of this compound. We first stu ...
Isolation and Characterization of Protease Inhibitors from Animal
... Protease inhibitors (PIs) are ubiquitous in nature and are produced by diverse animals as well as plants. There are different types of proteases such as cysteine proteases (such as papain from papayas), metalloproteases (such as thermolysin from bacteria), serine proteases (such as trypsin, chymotry ...
... Protease inhibitors (PIs) are ubiquitous in nature and are produced by diverse animals as well as plants. There are different types of proteases such as cysteine proteases (such as papain from papayas), metalloproteases (such as thermolysin from bacteria), serine proteases (such as trypsin, chymotry ...
Unusual ADP-forming acetyl-coenzyme A synthetases from the
... and catalytic features, from the mesophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. ACD from H. marismortui was purified and characterized as a saltdependent, mesophilic ACD of homodimeric structure (166 kDa). The encoding gene was ident ...
... and catalytic features, from the mesophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. ACD from H. marismortui was purified and characterized as a saltdependent, mesophilic ACD of homodimeric structure (166 kDa). The encoding gene was ident ...
Unconstrained Structure Formation in Coarse
... simulations. To cope with this difficulty, increasingly more accurate and sophisticated coarse-grained models are currently being developed. In the present thesis, we introduce a solvent-free coarse-grained model for proteins. Proteins are modeled by four beads per amino acid, providing enough backb ...
... simulations. To cope with this difficulty, increasingly more accurate and sophisticated coarse-grained models are currently being developed. In the present thesis, we introduce a solvent-free coarse-grained model for proteins. Proteins are modeled by four beads per amino acid, providing enough backb ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... order to obtain pure protein. More puzzling was the fact that the partially pure enzyme, which we did obtain, was always in th e b form rather than the a form. Insofar as we could determine we had followed the Coris’ procedure exactly except that we had clarified the original muscle extract by centr ...
... order to obtain pure protein. More puzzling was the fact that the partially pure enzyme, which we did obtain, was always in th e b form rather than the a form. Insofar as we could determine we had followed the Coris’ procedure exactly except that we had clarified the original muscle extract by centr ...
Structure and Function of Mammalian Ribosomes
... such an incubation mixture with 0.5 M-KCl, followed by sucrose gradient centrifugation in 0.3 M-KC1 and 0.002 or 0.003 M-magnesium acetate, 80 to 90% of all ribosomea are found as 60 s and 40 s subunits. These subunits can be concentrated from the gradient fractions by ethanol precipitation. The pur ...
... such an incubation mixture with 0.5 M-KCl, followed by sucrose gradient centrifugation in 0.3 M-KC1 and 0.002 or 0.003 M-magnesium acetate, 80 to 90% of all ribosomea are found as 60 s and 40 s subunits. These subunits can be concentrated from the gradient fractions by ethanol precipitation. The pur ...
1. dia
... The solubility of Fmoc-Asn-OH and Fmoc-Gln-OH is extremely bad. The Trt protecting group increases the solubility and prevents the dehydratation as well as ring closure side reactions during the synthesis. N-terminal Gln or Asn-Gly (Arg, Ser, Ala, Asn) sequence may cause problems after the cleavage ...
... The solubility of Fmoc-Asn-OH and Fmoc-Gln-OH is extremely bad. The Trt protecting group increases the solubility and prevents the dehydratation as well as ring closure side reactions during the synthesis. N-terminal Gln or Asn-Gly (Arg, Ser, Ala, Asn) sequence may cause problems after the cleavage ...
Synergistic Inhibitory Effects of Hypoxia and Iron
... were 33% higher in hypoxic (H) mice fed the FeA diet (H-FeA) compared with normoxic (N) mice fed the same diet (N-FeA). Hypoxic mice fed the FeD (H-FeD) became anemic, as indicated by reduced hemoglobin relative to that of N-FeA and H-FeA mice (Fig. 2A). Total liver iron concentrations trended to be ...
... were 33% higher in hypoxic (H) mice fed the FeA diet (H-FeA) compared with normoxic (N) mice fed the same diet (N-FeA). Hypoxic mice fed the FeD (H-FeD) became anemic, as indicated by reduced hemoglobin relative to that of N-FeA and H-FeA mice (Fig. 2A). Total liver iron concentrations trended to be ...
Fastness in Mycobacterium tuberculosis
... kinases (STPK) [2,3], suggesting that these two phospho-based signaling systems are of comparable importance in this microorganism. Knowledge of the substrates of each of the Mtb STPK is essential for understanding their function. Several kinase-substrates pairs have been identified and characterize ...
... kinases (STPK) [2,3], suggesting that these two phospho-based signaling systems are of comparable importance in this microorganism. Knowledge of the substrates of each of the Mtb STPK is essential for understanding their function. Several kinase-substrates pairs have been identified and characterize ...
Ethanol-soluble Intermediates and Products of
... acetate metabolism results in almost quantitative conversion of acetate to two products, CO, and the reserve polysaccharide, paramylon. Six hours after addition of p4C]acetate, these two products accounted for some 88 yo of the acetate carbon consumed, and much of the other 12% was in the form of et ...
... acetate metabolism results in almost quantitative conversion of acetate to two products, CO, and the reserve polysaccharide, paramylon. Six hours after addition of p4C]acetate, these two products accounted for some 88 yo of the acetate carbon consumed, and much of the other 12% was in the form of et ...
Unit One: Introduction to Physiology: The Cell and General
... into fatty acids and inhibits gluconeogenesis in the liver e. In the brain most of the cells are permeable to glucose and can use glucose without the intermediation of insulin ...
... into fatty acids and inhibits gluconeogenesis in the liver e. In the brain most of the cells are permeable to glucose and can use glucose without the intermediation of insulin ...
Fate of ammonium15N in a Norway spruce forest under long
... [%] of the microbial biomass, the fumigated extract and the unfumigated extract. Amino acids Hydrolysable amino acids (HAA) were extracted from ground soil (\200 lm), and quantified as the sum of enantiomers of individual amino acids following the method of Amelung and Zhang (2001). Briefly, the ami ...
... [%] of the microbial biomass, the fumigated extract and the unfumigated extract. Amino acids Hydrolysable amino acids (HAA) were extracted from ground soil (\200 lm), and quantified as the sum of enantiomers of individual amino acids following the method of Amelung and Zhang (2001). Briefly, the ami ...
Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea
... technological importance. The follicle is a convenient small-scale.structure for the study of the processes of growth, differentiation and keratinization, and as such is the subject of active biochemical and morphological investigations. This paper describes work on the tryptic digest of a urea-solu ...
... technological importance. The follicle is a convenient small-scale.structure for the study of the processes of growth, differentiation and keratinization, and as such is the subject of active biochemical and morphological investigations. This paper describes work on the tryptic digest of a urea-solu ...
The kinetics of the conversion of DHA to MGO
... Commun. 34, 3881-3894. Both acid (H+) and base (OH-) catalysis Note that the reaction that forms MGO is irreversible ...
... Commun. 34, 3881-3894. Both acid (H+) and base (OH-) catalysis Note that the reaction that forms MGO is irreversible ...
Metabolic Engineering for Production of Complex Lipids in Tobacco
... esterified to a glycerol backbone at the sn-1, sn-2 and sn-3 positions (Figure 3c). The fatty acids in a TAG molecule can differ, but are naturally a mixture of two or three different fatty acids. Most natural fats are composed of a mixture of simple and mixed triacylglycerols. In most organisms, TA ...
... esterified to a glycerol backbone at the sn-1, sn-2 and sn-3 positions (Figure 3c). The fatty acids in a TAG molecule can differ, but are naturally a mixture of two or three different fatty acids. Most natural fats are composed of a mixture of simple and mixed triacylglycerols. In most organisms, TA ...