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DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA
DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA

... Here we use the notation T/U, because genetic code is read from mRNA, and so we will not differ their determinative ability (“power”) in what follows. Now we introduce a numerical characteristics of the empirical “power” — determinative degree dx of nucleotide x and make transition from qualitative ...
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... k. Adjust the position of the Drop Counter so that the Microstirrer on the pH Sensor is just touching the bottom of the beaker. l. Adjust the reagent reservoir so its tip is just above the Drop Counter slot. 7. Turn on the magnetic stirrer so that the Microstirrer is stirring at a fast rate. 8. You ...
Amino acid transporters: roles in amino acid sensing and signalling
Amino acid transporters: roles in amino acid sensing and signalling

... gene transcripts (notably those involved in the biosynthesis or transport of amino acids) may be increased upon amino acid deprivation [13,20] by specific mechanisms which oppose the global changes in gene expression [21]. Our knowledge of the downstream signalling and geneexpression mechanisms modu ...
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Novel Specific Halogenating Enzymes from Bacteria
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... no substrate specificity at all (Franssen, 1994). However, as haloperoxidases were the only halogenating enzymes known, with the exception of some Sadenosyl methionine transferases that are involved in the formation of methyl halides (Wuosmaa & Hager, 1990), it was gene rally accepted th at these en ...
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... e. The size of the R-group determines the number of carbons left after deamination. B. Anabolism 1. ATP produced during catabolism drives anabolism. 2. Substrates making up pathways can be used as starting materials for synthetic reactions. 3. The molecules used for biosynthesis constitute the cell’ ...
C) the gain of electrons.
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... interfere with the cell's abilities to catalyze various reactions. C) Elevated body temperatures will increase the energy of activation needed to start various chemical reactions in the body. This will interfere with the ability of enzymes to catalyze vital chemical reactions. D) Elevated body tempe ...
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... • During glycolysis, ATP is produced by substrate level phosphorylation—by direct enzymatic transfer of a phosphate to ADP. Only a small amount of ATP is produced this way. • The enzyme that catalyzes the third step, phosphofructokinase (PFK), is an allosteric enzyme. It inhibits glycolysis when the ...
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... e. The size of the R-group determines the number of carbons left after deamination. B. Anabolism 1. ATP produced during catabolism drives anabolism. 2. Substrates making up pathways can be used as starting materials for synthetic reactions. 3. The molecules used for biosynthesis constitute the cell’ ...
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... B- β-Oxidation of fatty acids c. Sources of carnitine:  Carnitine can be obtained from the diet, where it is found primarily in meat products.  Carnitine can also be synthesized from the amino acids lysine and methionine by an enzymatic pathway found in the liver and kidney but not in skeletal or ...
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... An inorganic phosphate group from the cytosol is bonded to each of the newly created 1,3-bisphosphoglycerate. This process is mediated by triosephosphate Dehydrogenase. 7. The 2 molecules of 1,3-bisphosphoglycerate now will release one phosphate each to phosphorylate ADP to ATP (substrate-level phos ...
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... (NYITR-NH2 ) was allowed to react with DBEP. Brominecontaining ions (291/293 m/z) appeared again in the MS/MS spectrum together with a 70 m/z ion (immonium ion of N) and a series of *b ions (832, 676, 576, 463 m/z corresponding to labeled NYITR, NYIT, NYI, NY sequences) along with a series of *a ion ...
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Tutorial: Sequence-Based Analysis
Tutorial: Sequence-Based Analysis

... Positive Score: Conservative Substitution (e.g., Lys/Arg, +3) High Score for Identical Matches: Rare Amino Acids (e.g., Trp, Cys) ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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