Practical part
... Mix and incubate at the laboratory temperature for 10 minutes. Read the optical density of the sample at 510 nm against the blank. Calculate the quantity of pyruvate (in g) according to the formula: X = D / 0.09 , where X - quantity of pyruvate in g, D - Optical density. The calculation of transam ...
... Mix and incubate at the laboratory temperature for 10 minutes. Read the optical density of the sample at 510 nm against the blank. Calculate the quantity of pyruvate (in g) according to the formula: X = D / 0.09 , where X - quantity of pyruvate in g, D - Optical density. The calculation of transam ...
Embden-Meyerhof-Parnas Pathway
... • AMP is a better indicator than ADP due to the activity of adenylate kinase: ...
... • AMP is a better indicator than ADP due to the activity of adenylate kinase: ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
... Thus, 12 mutants resulting from site-directed mutagenesis have been characterized that have codon substitutions in a distinct site of the ecoRII gene. The expression of the mutant genes in E. coli cells resulted in mutant EcoRII proteins with point amino acid substitutions: Asp(110) → Lys, Asn, Thr, ...
... Thus, 12 mutants resulting from site-directed mutagenesis have been characterized that have codon substitutions in a distinct site of the ecoRII gene. The expression of the mutant genes in E. coli cells resulted in mutant EcoRII proteins with point amino acid substitutions: Asp(110) → Lys, Asn, Thr, ...
G5. Strategies for Stabilization of Enzymes in Organic
... Biodiversity prospecting can be utilized to isolate the enzymes that are staying functional under harsh conditions from living organisms. These so-called extremozymes are collected from microorganisms that can grow under extreme conditions. In these cases nature employs many different structural str ...
... Biodiversity prospecting can be utilized to isolate the enzymes that are staying functional under harsh conditions from living organisms. These so-called extremozymes are collected from microorganisms that can grow under extreme conditions. In these cases nature employs many different structural str ...
23 Amino Acids, Peptides, and Proteins
... essential amino acids from our diets because we either cannot synthesize them at all or cannot synthesize them in adequate amounts. For example, we must have a dietary source of phenylalanine because we cannot synthesize benzene rings. However, we do not need tyrosine in our diets, because we can sy ...
... essential amino acids from our diets because we either cannot synthesize them at all or cannot synthesize them in adequate amounts. For example, we must have a dietary source of phenylalanine because we cannot synthesize benzene rings. However, we do not need tyrosine in our diets, because we can sy ...
Chap. 4. "Proteins: Three-Dimensional Structure and Function
... pitch is 0.94 nm. Thus, the collagen helix is much more extended than an α helix. Individual polypeptides associate in a triple-helical supercoil called a collagen rod (3,000 Å long) in which the collagen molecules are coiled about each other in a right-handed triple helix (Fig. 4.36). Because colla ...
... pitch is 0.94 nm. Thus, the collagen helix is much more extended than an α helix. Individual polypeptides associate in a triple-helical supercoil called a collagen rod (3,000 Å long) in which the collagen molecules are coiled about each other in a right-handed triple helix (Fig. 4.36). Because colla ...
Lesson Overview
... What are the functions of each of the four groups of macromolecules? Living things use carbohydrates as their main source of energy. Plants, some animals, and other organisms also use carbohydrates for structural purposes. ...
... What are the functions of each of the four groups of macromolecules? Living things use carbohydrates as their main source of energy. Plants, some animals, and other organisms also use carbohydrates for structural purposes. ...
Pentose Phosphate Pathway - Berkeley MCB
... Ribulose 5-phosphate + Xylulose 5-phosphate Sedoheptulose 7-phosphate + Glyceraldehyde 3-phosphate (G3P) ...
... Ribulose 5-phosphate + Xylulose 5-phosphate Sedoheptulose 7-phosphate + Glyceraldehyde 3-phosphate (G3P) ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... employed. Furthermore, extracellular microbial metabolites, particularly of anaerobic bacteria, have been studied by GC. The resulting chromatographic patterns are usually specific as to genus or sometimes as to species (Larsson & Miirdh, 1977). One approach to the GC study of microbial constituents ...
... employed. Furthermore, extracellular microbial metabolites, particularly of anaerobic bacteria, have been studied by GC. The resulting chromatographic patterns are usually specific as to genus or sometimes as to species (Larsson & Miirdh, 1977). One approach to the GC study of microbial constituents ...
Potential Value of the Mormon Cricket (Orthoptera: Tettigoniidae
... Note that the chicks on the conventional corn-soybean meal diet (Diet l) grew more slowly than those on either corn-cricket diet (diets 2 and 3). Even without the addition of an antibiotic to the cricket diets some of this difference is probably due to the higher metabolizable energy content of the ...
... Note that the chicks on the conventional corn-soybean meal diet (Diet l) grew more slowly than those on either corn-cricket diet (diets 2 and 3). Even without the addition of an antibiotic to the cricket diets some of this difference is probably due to the higher metabolizable energy content of the ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... employed. Furthermore, extracellular microbial metabolites, particularly of anaerobic bacteria, have been studied by GC. The resulting chromatographic patterns are usually specific as to genus or sometimes as to species (Larsson & Miirdh, 1977). One approach to the GC study of microbial constituents ...
... employed. Furthermore, extracellular microbial metabolites, particularly of anaerobic bacteria, have been studied by GC. The resulting chromatographic patterns are usually specific as to genus or sometimes as to species (Larsson & Miirdh, 1977). One approach to the GC study of microbial constituents ...
Eris - Molecules in Action, LLC
... the protein as a weighted sum of van der Waals (VDW) interaction, solvation energy, hydrogen bonding, and backbone-‐ dependent statistical energy for any given amino acid and rotamer state. The unfolded stat ...
... the protein as a weighted sum of van der Waals (VDW) interaction, solvation energy, hydrogen bonding, and backbone-‐ dependent statistical energy for any given amino acid and rotamer state. The unfolded stat ...
free energy - Thunderbird High School
... Regulation of enzyme activity helps control metabolism • Chemical chaos would result if a cell’s metabolic pathways were not tightly regulated • A cell does this by switching on or off the genes that encode specific enzymes or by regulating the activity of enzymes ...
... Regulation of enzyme activity helps control metabolism • Chemical chaos would result if a cell’s metabolic pathways were not tightly regulated • A cell does this by switching on or off the genes that encode specific enzymes or by regulating the activity of enzymes ...
Sphingolipid homeostasis in the web of metabolic routes
... and, to a larger extent, for multicellular organisms. Indeed, many biosynthetic intermediates, in addition to the SL end products, are bioactive molecules whose accumulation or absence can severely influence cell functions [1–3]. As a consequence, several mechanisms contribute to the control of enzym ...
... and, to a larger extent, for multicellular organisms. Indeed, many biosynthetic intermediates, in addition to the SL end products, are bioactive molecules whose accumulation or absence can severely influence cell functions [1–3]. As a consequence, several mechanisms contribute to the control of enzym ...
Learning Objectives
... The Process of Cellular Respiration 7. Name the three stages of cellular respiration and state the region of the eukaryotic cell where each stage occurs. 8. Describe how glucose changes as it proceeds through glycolysis. 9. Explain why ATP is required for the preparatory steps of glycolysis. 10. Ide ...
... The Process of Cellular Respiration 7. Name the three stages of cellular respiration and state the region of the eukaryotic cell where each stage occurs. 8. Describe how glucose changes as it proceeds through glycolysis. 9. Explain why ATP is required for the preparatory steps of glycolysis. 10. Ide ...
The Tricarboxylic Acid Cycle in Thiobacillus
... Our results further substantiate the hypothesis that in most of the autotrophic organisms the tricarboxylic acid cycle is incomplete. In agreement with Taylor, Hoare & Hoare (I 969) we were unable to detect the a-ketoglutarate dehydrogenase in Thiobacillus denitrijicans. We also failed to demonstrat ...
... Our results further substantiate the hypothesis that in most of the autotrophic organisms the tricarboxylic acid cycle is incomplete. In agreement with Taylor, Hoare & Hoare (I 969) we were unable to detect the a-ketoglutarate dehydrogenase in Thiobacillus denitrijicans. We also failed to demonstrat ...
Synthesis characterization and pharmacological
... Reaction will be monitored by TLC and product purified by recrystllisation. b) Spectroscopic data: IR of all new compounds will be obtained by using spectrophotometers available in our college, NMR and mass spectroscopic data will obtain from I.I.Sc Bangalore and I.I.T. Madras etc. c) In vitro Anti- ...
... Reaction will be monitored by TLC and product purified by recrystllisation. b) Spectroscopic data: IR of all new compounds will be obtained by using spectrophotometers available in our college, NMR and mass spectroscopic data will obtain from I.I.Sc Bangalore and I.I.T. Madras etc. c) In vitro Anti- ...
Planta
... six-carbon citrate. The reaction is catalyzed by citrate synthase in the mitochondria, as part of the TCA cycle (Sadka et al. 2001). Regulation of the isomerization of citrate to isocitrate by aconitase, in the following metabolic step, plays a major role in citrate homeostasis (Bogin and Wallace 19 ...
... six-carbon citrate. The reaction is catalyzed by citrate synthase in the mitochondria, as part of the TCA cycle (Sadka et al. 2001). Regulation of the isomerization of citrate to isocitrate by aconitase, in the following metabolic step, plays a major role in citrate homeostasis (Bogin and Wallace 19 ...
Creation/Evolution - Geoscience Research Institute
... representing a few amino acids or possibly one codon representing a “group” of amino acids More precise codon meaning evolves perhaps with only the first two bases having meaning with discrimination at the third position evolving later The code becomes “frozen” when the system becomes so complex tha ...
... representing a few amino acids or possibly one codon representing a “group” of amino acids More precise codon meaning evolves perhaps with only the first two bases having meaning with discrimination at the third position evolving later The code becomes “frozen” when the system becomes so complex tha ...
Photosynthesis
... channel proteins (ATP synthase) in the cristae generate energy to drive the formation of ATP’s by allowing the protons to flow back into the matrix from the cristae. The process in which ATP is produced by the flow of protons across the channel is called oxidative phosphorylation. - NADH produces 3 ...
... channel proteins (ATP synthase) in the cristae generate energy to drive the formation of ATP’s by allowing the protons to flow back into the matrix from the cristae. The process in which ATP is produced by the flow of protons across the channel is called oxidative phosphorylation. - NADH produces 3 ...
Cellular Respiration: Harvesting Chemical Energy
... – Occurs on the inner membrane of the mitochondrion ...
... – Occurs on the inner membrane of the mitochondrion ...