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OVERVIEW OF LIPID METABOLISM
OVERVIEW OF LIPID METABOLISM

... The triglycerides in chylomicrons can be cleared by lipoprotein lipase (L) at the endothelial surface of capillaries. The resulting fatty acids can be: a) stored as fat in adipose tissue (Note: Triglycerides (fat) can also be made from excess glucose in the fed state); b) used for energy in any tiss ...
Cellular Respiration: Harvesting Chemical Energy
Cellular Respiration: Harvesting Chemical Energy

... – Occurs on the inner membrane of the mitochondrion ...
The effect of end product, phosphate, on the enzyme phosphatase
The effect of end product, phosphate, on the enzyme phosphatase

... Phosphatases are thus key enzymes in cell metabolism.There are two main groups of phosphatases, acid or alkaline depending on their optimum pH. This experiment involves an acid phosphatase extracted from germinating mung beans (beansprouts). The enzyme is also found in potatoes, tomato leaves, wheat ...
Protein Biosynthesis Translation
Protein Biosynthesis Translation

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a peptide bond forms that adds an amino acid
a peptide bond forms that adds an amino acid

... Ribosomes and the Mechanism of Translation • The ribosome is a molecular machine that synthesizes proteins in a three-step sequence. 1. An aminoacyl tRNA carrying the correct anticodon for the mRNA codon enters the A site. 2. A peptide bond forms between the amino acid on the aminoacyl tRNA in the ...
The Permeability Properties of Rat Liver Lysosomes to Nucleosides
The Permeability Properties of Rat Liver Lysosomes to Nucleosides

... whereas some smaller but less hydrophobic molecules probably cannot. It seems likely therefore that nucleic acids are broken down by lysosomal enzymes to the nucleosides, which then escape intact from the lysosome. It is extremely unlikely that nucleotides could pass across the lysosomal membrane [m ...
Energy Conversion Pathways 1. Substrate level phosphorylation
Energy Conversion Pathways 1. Substrate level phosphorylation

... 28. a) The radioactive carbon became incorporated into all of the cycle intermediates at atom positions that could only be explained by products also serving as substrates. The metabolism of oxaloacetate in one round of the cycle produces a different radioactively labeled product depending on the nu ...
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Protein and Glycoprotein Characterisation by Mass
Protein and Glycoprotein Characterisation by Mass

... scrambling due to oxido-reductive events, and also the sheer complexity of possible structures in cases where the protein Cysteine content is high causes interpretation problems of its own. Figure 5A, 5B and 5C illustrates the strategy developed for disulphide bridge analysis, where it should be app ...
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Stable nitrogen isotopic fractionation associated with transamination

Final Report
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4 Dr. M. Alzaharna 2016 Dr. M. Alzaharna 2016 II. REACTIONS OF
4 Dr. M. Alzaharna 2016 Dr. M. Alzaharna 2016 II. REACTIONS OF

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08_Lecture_Presentation_PC

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Origin and evolution of peptide-modifying
Origin and evolution of peptide-modifying

... derived secondary metabolites. Based on this, we propose that peptide-modifying activities are likely to be the ancestral feature of the jumonji-related superfamily. Three distinct lineages of jumonji-like enzymes emerged in bacteria within the context of these biosynthetic systems and were transfer ...
PDF | 816.8KB - New Jersey Center for Teaching and Learning
PDF | 816.8KB - New Jersey Center for Teaching and Learning

... 37. Fat, waxes, oils, and steroids all fall under the category of lipids. Two common lipids are phospholipids and triacylglycerol. Explain how their structure differs and how this affects the molecules when they come in contact with water. Homework 38. How does hydrolysis participate in energy stor ...
Enzymes - HKEdCity
Enzymes - HKEdCity

... substrates, increasing the substrate concentration cannot increase the rate of reaction. This is because any extra substrate has to wait for the E- S complex dissociated into products and free enzyme. Ø Therefore at high substrate levels, both enzyme concentration and the dissociation time are the l ...
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Engineering of Aromatic Amino Acid Metabolism in

... The main application field of amino acids nowadays is in food. About fifty percent of amino acids manufactured worldwide are applied in human food production (Fig. 2). The amino acids with the largest market volumes are glutamic acid, lysine and methionine (64) (Table 1). The microbial production of ...
Calculation of the Free Energy of Solvation for Neutral Analogs of
Calculation of the Free Energy of Solvation for Neutral Analogs of

... a few.1 A common feature of many of these biophysical processes is the partitioning of specific functional groups between different environments. For example, during folding, amino acids within a protein must partition between a highly polarizable aqueous environment and the predominantly hydrophobi ...
Conceptual Questions C1. Answer: The start codon begins at the
Conceptual Questions C1. Answer: The start codon begins at the

... anticodon sequence that recognizes the codon sequence in mRNA. At the 3′ end, there is an acceptor stem, with the sequence CCA, that serves as an attachment site for an amino acid. Most tRNAs also have base modifications that occur within their nucleotide sequences. C13. Answer: They are very far ap ...
rll 24.5 The citric ocid cycle
rll 24.5 The citric ocid cycle

... This is what happens in the citric acid cycle: 1. Acetyl CoA and oxaloacetatecombine to form citrate. 2. Citric acid eventually loses two carbon atoms as carbon dioxide. The carbons in the two molecules of carbon dioxide are not the same carbons that entered the citric acid cycle as acetyl groups of ...
biomass composition
biomass composition

Movsumov I.S., Garayev E.A. STUDYING OF CHEMICAL
Movsumov I.S., Garayev E.A. STUDYING OF CHEMICAL

... The influence of addition of amino acids before the drying of mushroom (Agaricus bisporus L.) on the composition of aroma compounds was studied by the capillary gas chromatography and chromatography – mass spectrometry methods. It was found that concentration of carbonyls and heterocyclic volatile c ...
Nature: Serine is a natural ligand and allosteric activator of pyruvate
Nature: Serine is a natural ligand and allosteric activator of pyruvate

... deprivation decreases PKM2 activity in cells, such that more glucosederived carbon is channelled into serine and glycine biosynthesis. PKM2 is a tightly regulated enzyme that responds not only to the availability of PEP and ADP substrates, but also to the upstream ...
DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA
DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA

... Here we use the notation T/U, because genetic code is read from mRNA, and so we will not differ their determinative ability (“power”) in what follows. Now we introduce a numerical characteristics of the empirical “power” — determinative degree dx of nucleotide x and make transition from qualitative ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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