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Lecture-1-molbio
Lecture-1-molbio

... Translation of mRNA to Protein • Each triplet is called a codon • The code is degenerate –61 codons map to 20 amino acids –Between 1 and 6 codons per amino acid –3 codons stop translation (TAA, TGA, TAG) –Codons for the same amino acid are called synonymous –DNA mutations that do not change the ami ...
NucleicAcids
NucleicAcids

... • During preparations for cell division each of the strands serves as a template to order nucleotides into a new complementary strand. • This results in two identical copies of the original double-stranded DNA molecule. • The copies are then distributed to the daughter cells. ...
Slide 1
Slide 1

... Figure 1 Genes used to study RNA-mediated genetic interference in C.elegans. Intron–exon structure for genes used to test RNA-mediated inhibition are shown (grey and filled boxes, exons; open boxes, introns; patterned and striped boxes, 5' and 3' untranslated regions. unc-22. ref. 9, unc-54, ref. 1 ...
Genetic Diseases
Genetic Diseases

... – Gene is only on X chromosome, males are affected more than females because they only have on X chromosome ...
Introduction
Introduction

... PCR to amplify a 132bp region of exon 8 containing the mutation causative for achondroplasia was carried out on 5, 10 or 20µl of DNA extracted from 400µl or 800µl of plasma, as well as on genomic DNA from an unaffected and a positive control. On an unaffected DNA sample, restriction digest of the PC ...
Chapter 7 Manipulating Proteins, DNA, and RNA
Chapter 7 Manipulating Proteins, DNA, and RNA

... To prepare tagged knockout mutants ...
The present genetic tests
The present genetic tests

... sorbose-complete. on ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 9. Particle gun bombardment technique cannot be used for gene transfer in plants 10. Haploid set of chromosome (n) of an organism is termed as genome ...
Pre-exam 2
Pre-exam 2

... follow  along.  Before  you  begin,  ask  yourself  these  questions:   • Do  you  understand  how  to  identify  homologous  chromosomes?     • Do  you  understand  the  relationship  between  the  two  chromatids  that  make  up  a  rep ...
Untitled
Untitled

... Most retrovirus-induced cancers occur when an onc gene is in their genome. ...
CENTRAL DOGMA AND GENE REGULATION
CENTRAL DOGMA AND GENE REGULATION

... Codon: the triplicate code found on mRNA that codes for each of the 20 amino acids, for start (methionine) and stop Genetic Code: the inventory of linkages between nucleotide triplets and the amino acids they code for: GENE REGULATION: Determines when a protein is expressed (produced) in a cell. Som ...
Answers to Mastering Concepts Questions
Answers to Mastering Concepts Questions

... production. If proteins are made they can be inactivated by not folding correctly or by being degraded. 16. What events occur in each of the five stages of viral replication? 1) Attachment -- virus adheres to host cell receptor. 2) Penetration -- virus enters the cell. 3) Synthesis -- multiple copie ...
Index case of acute myeloid leukemia in a family
Index case of acute myeloid leukemia in a family

... Given the persistence of CEBPA c.442G.T at the time of CR, we postulated that it was a germ line mutation. We confirmed this by conducting Sanger sequencing of the CEBPA gene in genomic DNA isolated from skin fibroblasts (Figure 1A). This is a nonsense mutation located downstream of the p30 start co ...
Recombinant DNA - Richmond School District
Recombinant DNA - Richmond School District

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Genomic differences study questions
Genomic differences study questions

... Addition/deletion of DNA Point mutation Keeping track of gene variants – Concepts of ortholog / paralog ...
Document
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... c. is caused by the transfer of cells from one organism to another. d. results from mutations that destroy normal gene functioning. _____ 2. Homeoboxes are a. found only in prokaryotes. b. found only in Drosophila. ...
Endocrinology 3
Endocrinology 3

... Viral Rearrangement - viruses can become lysogenic and excise and carry genes or foreign promoter DNA to subsequent cellular hosts where these insert into nonhomologous sites and are expressed in a non-regulated or inappropriately regulated fashion, often leading to oncogenesis via disruption of the ...
CM - Overview of HL7V2 genetic report lite for LOINC Lab commitee
CM - Overview of HL7V2 genetic report lite for LOINC Lab commitee

... • We have divided the world into simple, complex, structural and pharmacogenomic variants. • The simple variant is a change in the DNA that is contiguous over a small (< 50 nucleotide) range of the genome. Mostly these are changes in one or two nucleotides. A major share of the clinically important ...
2. If 20% of the DNA in a guinea pig cell is adenine, what
2. If 20% of the DNA in a guinea pig cell is adenine, what

... make the remaining three types of mRNA made up of only one type of nucleotide—that is, poly G, poly U, or poly C. Then make all possible combinations of the nucleotides taken two at a time—for example, GCGC, CGCG, AGAG, and so on. Next, make other combinations of nucleotides taken three at a time— f ...
HONORS BIOLOGY FINAL EXAM REVIEW PACKET AND FORMAT
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... DNA REPLICATIONOCCURS AT REPLICATION FORKS, USES DNA POLYMERASE WHICH CAN ONLY WORK IN ONE DIRECTION, NITROGEN BASES ARE ADDED AT REPLICATION FORKS, ERRORS CAN BE CORRECTED USING DNA LIGASE AND PROOFREADER ENZYMES, THE DNA STRAND IS NOT DISSEMBLED WHEN CORRECTING FOR ERRORS MDOUBLE CHROMOSOMES ARE ...
Genetic engineering
Genetic engineering

... well as among different species. DNA sequences within most genes contain the information to direct the order of amino acids within polypeptides according to the genetic code. In the code, a three-base sequence specifies one particular amino acid among the 20 possible choices. One or more polypeptide ...
Slide 1
Slide 1

... There are 4 types of base molecules: Adenine--Thiamine Cytosine--Guanine ...
DNA Transcription and Translation
DNA Transcription and Translation

... Translation ­ After transcription, the mRNA leaves the nucleus and enters the cytoplasm of the cell  to be translated ...
DNA, RNA, and Protein
DNA, RNA, and Protein

... living things. Some have modifications. o o o • Amino acids form 1 , 2 & 3 protein structures – Structures are essential to protein function ...
200 THINGS TO KNOW AP Biology TEST
200 THINGS TO KNOW AP Biology TEST

... 5. Adaptive radiation: organisms adapt to new niches w/in the same habitat due to Selective pressures 6. Spermatogenesis vs Oogenesis 7. Cohesive and Adhesive factors of water (also high specific heat) 8. Respiration vs Transpiration vs perspiration 9. BOD and Dissolved oxygen 10. Agonistic behavior ...
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Point mutation



A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.
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