What is Sequence Alignment?
... DNA or protein sequences • Not good for: generating alignment of closely related sequences Global and local alignments are fundamentally similar and differ only in optimization strategy used in aligning similar residues BCB 444/544 F07 ISU ...
... DNA or protein sequences • Not good for: generating alignment of closely related sequences Global and local alignments are fundamentally similar and differ only in optimization strategy used in aligning similar residues BCB 444/544 F07 ISU ...
Genetic Transformation of Poinsettia (Euphórbia
... chimera plants with the L1-layer colourless (pink), L1 and L2 colourless (marbled) or all three cell layers colourless (white) (Preil, W 1994). A light pink variety of poinsettia, ‘Princettia’, is already promoted as an autumn plant (Ladstein, pers. comm.). As poinsettia is a traditional Christmas p ...
... chimera plants with the L1-layer colourless (pink), L1 and L2 colourless (marbled) or all three cell layers colourless (white) (Preil, W 1994). A light pink variety of poinsettia, ‘Princettia’, is already promoted as an autumn plant (Ladstein, pers. comm.). As poinsettia is a traditional Christmas p ...
Natural Computing - Computer Science
... of a regular grid of cells • Space and time and discrete • Each cell can be in a finite number of states • Each cell changes its state according to a list of transition rules, based on its current state and the states of its neighbours • The grid updates its configuration synchronously Lila Kari, Un ...
... of a regular grid of cells • Space and time and discrete • Each cell can be in a finite number of states • Each cell changes its state according to a list of transition rules, based on its current state and the states of its neighbours • The grid updates its configuration synchronously Lila Kari, Un ...
BIOCHEMISTRY Which of the following single
... B. A-T base pairs exhibit less electron clouds around them C. G-C base pairs contain more hydrogen bonds than A-T pairs D. the phosphodiester bonds among G-C base pairs are weaker ...
... B. A-T base pairs exhibit less electron clouds around them C. G-C base pairs contain more hydrogen bonds than A-T pairs D. the phosphodiester bonds among G-C base pairs are weaker ...
BIOSENSOR (General principles and applications)
... charge is detected using ion-selective electrodes, such as pH-meters. ...
... charge is detected using ion-selective electrodes, such as pH-meters. ...
Structure-Guided Site-Directed Mutagenesis of the Bacterial ATP
... analyze the epsilon-inhibited structure of the F1 complex. Individual amino acids in the sequence were studied based on distances between individual atoms, angles/torsions, hydrogen bonds, and any clashes/contacts that may be important in the structure and function of the F1 complex. I identified tw ...
... analyze the epsilon-inhibited structure of the F1 complex. Individual amino acids in the sequence were studied based on distances between individual atoms, angles/torsions, hydrogen bonds, and any clashes/contacts that may be important in the structure and function of the F1 complex. I identified tw ...
DNA cytosine methylation in plant development
... H3K9 methyltransferase that stabilizes cytosine methylation, particularly in CHG trinucleotides. Although SUVH4/KYP is the major H3K9 methyltransferase in Arabidopsis, two related histone methyltransferases, SUVH5 and SUVH6, are needed for maintaining non-CG methylation at specific loci (Huettel et ...
... H3K9 methyltransferase that stabilizes cytosine methylation, particularly in CHG trinucleotides. Although SUVH4/KYP is the major H3K9 methyltransferase in Arabidopsis, two related histone methyltransferases, SUVH5 and SUVH6, are needed for maintaining non-CG methylation at specific loci (Huettel et ...
New lysosomal acid lipase gene mutants explain the phenotype of
... point mutations resulting in single amino acid substitutions, and small insertions or deletions that introduce stop codons have also been reported (4, 7, 9–14). These different mutations and/or their heteroallelic combinations could be responsible for the variable phenotypes observed. Although it ha ...
... point mutations resulting in single amino acid substitutions, and small insertions or deletions that introduce stop codons have also been reported (4, 7, 9–14). These different mutations and/or their heteroallelic combinations could be responsible for the variable phenotypes observed. Although it ha ...
Rapid enzyme assays investigating the variation in the glycolytic
... (in triplicate with their controls) at 25°C for 3 rain using a temperaturecontrolled 96-wetl microplate spectrophotometer (Molecular Devices, Menlo Park, CA). Enzyme activity was measured at 25°C because we are using maximal activity solely as an index of enzyme concentration. Thus it is necessary o ...
... (in triplicate with their controls) at 25°C for 3 rain using a temperaturecontrolled 96-wetl microplate spectrophotometer (Molecular Devices, Menlo Park, CA). Enzyme activity was measured at 25°C because we are using maximal activity solely as an index of enzyme concentration. Thus it is necessary o ...
Evolutionary origin and consequences of uniparental mitochondrial
... but no viable pollen. Much genetic and population genetic research on this trait has been carried out in natural populations of Plantago and Thymus, as well as in several agricultural crops. At first sight, the presence of appreciable frequencies of plants deficient in their male function is puzzlin ...
... but no viable pollen. Much genetic and population genetic research on this trait has been carried out in natural populations of Plantago and Thymus, as well as in several agricultural crops. At first sight, the presence of appreciable frequencies of plants deficient in their male function is puzzlin ...
Comparison of the Functional Differences for the Homologous Residues within... Carboxy Phosphate and Carbamate Domains of Carbamoyl Phosphate Synthetase
... was constructed based upon amino acid sequence alignments and the previously determined threedimensional structures of two mechanistically related proteins, biotin carboxylase and D-alanine:D-alanine ligase. The model was tested by mutation of ten amino acid residues predicted to be important for bi ...
... was constructed based upon amino acid sequence alignments and the previously determined threedimensional structures of two mechanistically related proteins, biotin carboxylase and D-alanine:D-alanine ligase. The model was tested by mutation of ten amino acid residues predicted to be important for bi ...
Lecture-Mic 623-Plasmids-Listeria - Home
... into four groups, the smallest being 5% of cultures resistant to both agents, and the largest (53%) being sensitive to cadmium and resistant to arsenic. The resistance patterns to these agents and the presence of plasmid DNA varied markedly between the serotypes of the cultures. The detection of pla ...
... into four groups, the smallest being 5% of cultures resistant to both agents, and the largest (53%) being sensitive to cadmium and resistant to arsenic. The resistance patterns to these agents and the presence of plasmid DNA varied markedly between the serotypes of the cultures. The detection of pla ...
401 Prosiding Forum Inovasi Teknologi Akuakultur 2015 DIGESTIVE
... couldaffected the production of enzymes produced by a gland found in the digestive organs such as intestines, pancreas, stomach, and intestinal wall. The time where high digestive enzymes activity occured could be used as a basic references to applyartificial feed. Accordingto Gawlickka et al. (2000 ...
... couldaffected the production of enzymes produced by a gland found in the digestive organs such as intestines, pancreas, stomach, and intestinal wall. The time where high digestive enzymes activity occured could be used as a basic references to applyartificial feed. Accordingto Gawlickka et al. (2000 ...
biology 160 laboratory objectives practical one
... BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO ...
... BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO ...
(lip) that - Repositories
... Exogenous lipoic acid may be taken up and incorporated into intracellular pools by a constitutive, energy-dependent active transport system which is similar to those for the amino acids glycine and proline. The membrane-bound transport proteins are capable of concentrating lipoic acid up to 100-fold ...
... Exogenous lipoic acid may be taken up and incorporated into intracellular pools by a constitutive, energy-dependent active transport system which is similar to those for the amino acids glycine and proline. The membrane-bound transport proteins are capable of concentrating lipoic acid up to 100-fold ...
Introduction to Bioinformatics
... • building blocks for proteins (20 different) • vary by side chain groups • Hydrophilic amino acids are water soluable • Hydrophobic are not • Linked via a single chemical bond (peptide bond) • Peptide: Short linear chain of amino acids (< 30) polypeptide: long chain of amino acids (which can be upw ...
... • building blocks for proteins (20 different) • vary by side chain groups • Hydrophilic amino acids are water soluable • Hydrophobic are not • Linked via a single chemical bond (peptide bond) • Peptide: Short linear chain of amino acids (< 30) polypeptide: long chain of amino acids (which can be upw ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... This SALSA® MLPA® kit is for basic research! This kit enables you to detect aberrant methylation of CpG islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible ...
... This SALSA® MLPA® kit is for basic research! This kit enables you to detect aberrant methylation of CpG islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible ...
02 Cholesterol Metabolism2012-03-18 01:50617 KB
... • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage-activating protein (SCAP) is an intracellular cholesterol sensor ...
... • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage-activating protein (SCAP) is an intracellular cholesterol sensor ...
Redalyc.Influence of Mg2+ ions on the interaction between 3,5
... inhibitors have bee reported. However, up-to-date, there are no reports on the successful control of infection (9). HTLV-I Integrase (IN) is the enzyme responsible for the integration of reversely transcribed viral DNA (cDNA) into host cell DNA through two-step metal dependent reactions (10). In add ...
... inhibitors have bee reported. However, up-to-date, there are no reports on the successful control of infection (9). HTLV-I Integrase (IN) is the enzyme responsible for the integration of reversely transcribed viral DNA (cDNA) into host cell DNA through two-step metal dependent reactions (10). In add ...
BIOCHEMISTRY
... Biochemistry can be defined as the science concerned with the chemical basis of life. The cell is the structural unit of living systems. Thus, biochemistry can also be described as the science concerned with the chemical constituents of living cells and with reactions and processes they undergo. By ...
... Biochemistry can be defined as the science concerned with the chemical basis of life. The cell is the structural unit of living systems. Thus, biochemistry can also be described as the science concerned with the chemical constituents of living cells and with reactions and processes they undergo. By ...
Artificial selection shifts flowering phenology and other correlated
... number of days from the end of the vernalization treatment to first flower. For each base population, the earliest flowering 20% (E), the latest flowering 20% (L) and a random control group 20% (C) were used to produce six selection lines: one upward, one downward and one control per replicate. The ...
... number of days from the end of the vernalization treatment to first flower. For each base population, the earliest flowering 20% (E), the latest flowering 20% (L) and a random control group 20% (C) were used to produce six selection lines: one upward, one downward and one control per replicate. The ...
Questions & Answer keys Test 2 Genetic engg.
... a. Pasta filata - type cheese b. Blue - veined cheese c. Swiss cheese d. Hard cheese ...
... a. Pasta filata - type cheese b. Blue - veined cheese c. Swiss cheese d. Hard cheese ...
Molecular Cloning of Dog Mast Cell Tryptase and a Related Protease
... Cloning of Dog Mast Cell Tryptase. The BR dog mastocytoma cDNA library contains 2 X lo5 unique recombinants. Approximately lo6 plaques from the amplified library were screened with an oligonucleotide probe based on the N-terminal amino acid sequence of dog tryptase. Of 27 hybridizing clones, 10 were ...
... Cloning of Dog Mast Cell Tryptase. The BR dog mastocytoma cDNA library contains 2 X lo5 unique recombinants. Approximately lo6 plaques from the amplified library were screened with an oligonucleotide probe based on the N-terminal amino acid sequence of dog tryptase. Of 27 hybridizing clones, 10 were ...
Metabolism of Members of the Spiroplasmataceae
... contaminating and interfering enzymes, or perhaps, in certain cases, failure to induce an inducible enzyme. Furthermore, we cannot be certain that the reaction sequences which we detected in our in vitro studies with cell-free extracts are functional in actively metabolizing whole cells; such functi ...
... contaminating and interfering enzymes, or perhaps, in certain cases, failure to induce an inducible enzyme. Furthermore, we cannot be certain that the reaction sequences which we detected in our in vitro studies with cell-free extracts are functional in actively metabolizing whole cells; such functi ...
Deoxyribozyme
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.