EVMS Biosafety Procedure Manual
... the containment and biosafety practices outlined in the NIH Guidelines must be reported. Minor spills of low-risk agents not involving a breach of containment that were properly cleaned and decontaminated generally will not require reporting to a federal agency but is at the discretion of the IBC. C ...
... the containment and biosafety practices outlined in the NIH Guidelines must be reported. Minor spills of low-risk agents not involving a breach of containment that were properly cleaned and decontaminated generally will not require reporting to a federal agency but is at the discretion of the IBC. C ...
Assaying … promoter activity
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
video slide - Dublin City Schools Home
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
Optimal estimation of diffusion coefficients from single
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
Kinetic studies of human tyrosyl
... Wild-type and mutant human TDP enzymes were expressed in E. coli TOP10 cells bearing pBAD/Thio-huTDP, pBAD/Thio-huTDPND39 and pBAD/Thio-huTDPND174, respectively. After induction with 0.02% arabinose for 2 h, E. coli cells were pelleted and broken in lysis buffer by sonication. Cell lysate was separa ...
... Wild-type and mutant human TDP enzymes were expressed in E. coli TOP10 cells bearing pBAD/Thio-huTDP, pBAD/Thio-huTDPND39 and pBAD/Thio-huTDPND174, respectively. After induction with 0.02% arabinose for 2 h, E. coli cells were pelleted and broken in lysis buffer by sonication. Cell lysate was separa ...
PDF File - Computational Biochemistry Group
... the protein was confirmed by N-terminal sequencing and screening against the unfinished genome sequence from C. difficile strain 630 (epidemic type X). The high-score ORF coded for a 902 amino acid protein with a predicted molecular mass of 101,294 Da and corroborated the value obtained for the puri ...
... the protein was confirmed by N-terminal sequencing and screening against the unfinished genome sequence from C. difficile strain 630 (epidemic type X). The high-score ORF coded for a 902 amino acid protein with a predicted molecular mass of 101,294 Da and corroborated the value obtained for the puri ...
CURRICULUM VITAE Name: Antonella Spinazzola Nationality
... mitochondrial disorder. Science. 1999,83:689-92. The study showed that MNGIE results from a deficiency of thymidine phosphorylase, an enzyme that catalyzes the reversible breakdown of thymidine to thymine plus deoxyribose-1-phosphate. The report underlies the importance of understanding the nature a ...
... mitochondrial disorder. Science. 1999,83:689-92. The study showed that MNGIE results from a deficiency of thymidine phosphorylase, an enzyme that catalyzes the reversible breakdown of thymidine to thymine plus deoxyribose-1-phosphate. The report underlies the importance of understanding the nature a ...
Ribotyping of Clostridium perfringens from industrially produced
... Among 111 Cl. perfringens isolates from ground meat 107 distinctly different ribotype patterns were detected. In only four cases two Cl. perfringens isolates showed an identical ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl ...
... Among 111 Cl. perfringens isolates from ground meat 107 distinctly different ribotype patterns were detected. In only four cases two Cl. perfringens isolates showed an identical ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl ...
1 The Empirical Non-Equivalence of Genic and Genotypic Models of
... comparing genotypic s and genic s*. It is not. We can make the same sort of comparison between two different sorts of genotypic equilibria. At the sort of equilibrium relevant to our discussion selection actively maintains the equilibrium value. By that we mean that selection is acting at the equili ...
... comparing genotypic s and genic s*. It is not. We can make the same sort of comparison between two different sorts of genotypic equilibria. At the sort of equilibrium relevant to our discussion selection actively maintains the equilibrium value. By that we mean that selection is acting at the equili ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
... The biogenesis of heterochromatic siRNA requires specific members of the RDR, DCL, and AGO gene families. In the current model, plant-specific PolIV generates an aberrant RNA from methylated DNA templates (Figure 1.2) (Mosher et al. 2008). This aberrant RNA is then converted into dsRNA by RDR2 and ...
... The biogenesis of heterochromatic siRNA requires specific members of the RDR, DCL, and AGO gene families. In the current model, plant-specific PolIV generates an aberrant RNA from methylated DNA templates (Figure 1.2) (Mosher et al. 2008). This aberrant RNA is then converted into dsRNA by RDR2 and ...
NAD (H) Linked Enzyme Catalyzed Reactions using Coupled
... found to be of much use at commercial scale. Efficient regeneration and reuse of cofactors are essential for large scale synthetic applications of cofactor based enzymes [4]. Therefore, to maintain cost effectiveness in the use of cofactor-based enzymes at commercial scales, it is necessary to devel ...
... found to be of much use at commercial scale. Efficient regeneration and reuse of cofactors are essential for large scale synthetic applications of cofactor based enzymes [4]. Therefore, to maintain cost effectiveness in the use of cofactor-based enzymes at commercial scales, it is necessary to devel ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... Biology of Picornaviruses (Urbino, Italy, 1983) (Rueckert & Wimmer, 1984) has been used in this paper. Where reference is made to papers that used the earlier nomenclature, the former names of the respective proteins are given in parentheses for ease of reference.] The genomic RNA acts as a monocist ...
... Biology of Picornaviruses (Urbino, Italy, 1983) (Rueckert & Wimmer, 1984) has been used in this paper. Where reference is made to papers that used the earlier nomenclature, the former names of the respective proteins are given in parentheses for ease of reference.] The genomic RNA acts as a monocist ...
Nanosecond Time-Dependent Stokes Shift at the Tunnel
... was happening on the shorter time scales. The cause for such disparate results may originate from the fact that some fraction of the dye molecules was nonspecifically bound to the investigated protein. As the recorded TDSS is highly dependent on the distribution of the probe locations, the labeling ...
... was happening on the shorter time scales. The cause for such disparate results may originate from the fact that some fraction of the dye molecules was nonspecifically bound to the investigated protein. As the recorded TDSS is highly dependent on the distribution of the probe locations, the labeling ...
Kinetics and mechanisms of reactions catalyzed by
... stability and activity of lipases under the alkaline conditions normally prevailing in washing processes. Protein design and genetic engineering have made it possible to produce various types of lipases in economically attractive ways, and the detergent industry has benefited from such commercial de ...
... stability and activity of lipases under the alkaline conditions normally prevailing in washing processes. Protein design and genetic engineering have made it possible to produce various types of lipases in economically attractive ways, and the detergent industry has benefited from such commercial de ...
Predictions of Patterns of Response to Artificial Selection
... something of the genetic architecture in the natural population from which they were derived. We modeled artificial selection in samples drawn from natural populations in which variation had been maintained by recurrent mutation, with genes having an effect on the trait, which was subject to real st ...
... something of the genetic architecture in the natural population from which they were derived. We modeled artificial selection in samples drawn from natural populations in which variation had been maintained by recurrent mutation, with genes having an effect on the trait, which was subject to real st ...
Couchioplanes caeruleus - International Journal of Systematic and
... Nacalai Tesque, Inc., Kyoto, Japan); 0.2 M ammonium phosphate-acetonitrile (40:l) was used as the mobile phase. DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate ...
... Nacalai Tesque, Inc., Kyoto, Japan); 0.2 M ammonium phosphate-acetonitrile (40:l) was used as the mobile phase. DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate ...
Vapor phase alkylation of toluene using various
... Vapor phase alkylation of toluene using various alcohols over..., A. ABBASPOURRAD, et al., of effluent problems. 4 Non-conventional solid acid catalysts such as MCM-41 have been used in this area and they showed good thermal stability. 5,6 The use of zeolites as non-conventional Friedel-Crafts cataly ...
... Vapor phase alkylation of toluene using various alcohols over..., A. ABBASPOURRAD, et al., of effluent problems. 4 Non-conventional solid acid catalysts such as MCM-41 have been used in this area and they showed good thermal stability. 5,6 The use of zeolites as non-conventional Friedel-Crafts cataly ...
History of Discoveries in Molecular Biology
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
In-situ Protocol for larval/pupal/adult tissues
... would produce the sense or antisense probe, and then pick a unique restriction site in the T7 linker arm to cut. 2. Cut ~20 µL (10 ng) of a miniprep with a single enzyme to linearize. We typically incubate for 2 hours at 37°C to ensure the digestion goes to completion. The digestion volume should be ...
... would produce the sense or antisense probe, and then pick a unique restriction site in the T7 linker arm to cut. 2. Cut ~20 µL (10 ng) of a miniprep with a single enzyme to linearize. We typically incubate for 2 hours at 37°C to ensure the digestion goes to completion. The digestion volume should be ...
X chromosome inactivation- Review
... region in Xist gene and is required for X inactivation Model- Mutually exclusive binding of blocking factor to Xce on one X, and of initiator factor to Xist on other X Marsupials and in mice extraembyonic tissues- paternal X always inactivated in females ...
... region in Xist gene and is required for X inactivation Model- Mutually exclusive binding of blocking factor to Xce on one X, and of initiator factor to Xist on other X Marsupials and in mice extraembyonic tissues- paternal X always inactivated in females ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.