Isoenzymes in Clinical Diagnosis
... Two groups of phosphatase were originally detected by their different pH optima and called "acid" and "alkaline" phosphatase. Further "isoenzymes" of phosphatase were differentiated by susceptibility to inhibition by tartrate and other chemicals.'6 Different pepsins were separated by differences in ...
... Two groups of phosphatase were originally detected by their different pH optima and called "acid" and "alkaline" phosphatase. Further "isoenzymes" of phosphatase were differentiated by susceptibility to inhibition by tartrate and other chemicals.'6 Different pepsins were separated by differences in ...
... factors. We know now that variation in epigenetic marks between two MZ twins [63–65] can also explain phenotypic differences. MZ twins are derived from the same one-cell zygote, thus, share not only their genomic sequence but also the same initial epigenetic factors except for egg cleavage asymmetry. ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... gene from Pseudomonas putida ATCC11172 by PCR for constructing the chimeric HPO library (HPO11172). HPO11172 showed each single open-reading frame of 828 base pairs coding for 276 amino acids, respectively, and showed 87% similarity with P. putida IF-3 sequences. Approximately 600 transformants scre ...
... gene from Pseudomonas putida ATCC11172 by PCR for constructing the chimeric HPO library (HPO11172). HPO11172 showed each single open-reading frame of 828 base pairs coding for 276 amino acids, respectively, and showed 87% similarity with P. putida IF-3 sequences. Approximately 600 transformants scre ...
An rpoB signature sequence provides unique resolution for the
... level of analysis because of automatic gap removal. The procedure was repeated on each new well-supported subgroup (bootstrap value .50 %) until all the sequences realigned possessed exactly the same signature sequence and thus maximal resolution was achieved. For cluster V, the number of nucleotide ...
... level of analysis because of automatic gap removal. The procedure was repeated on each new well-supported subgroup (bootstrap value .50 %) until all the sequences realigned possessed exactly the same signature sequence and thus maximal resolution was achieved. For cluster V, the number of nucleotide ...
Dot Plot - APBioNET
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
... the intermolecular transglycosylation activity of ASase has gained increasing attention because it uses a relatively cheap substrate, sucrose, as well as its broad range of acceptor specificity [7, 8]. ASase can employ not only various glycones such as salicin [9] and arbutin [10] but also numerous ...
... the intermolecular transglycosylation activity of ASase has gained increasing attention because it uses a relatively cheap substrate, sucrose, as well as its broad range of acceptor specificity [7, 8]. ASase can employ not only various glycones such as salicin [9] and arbutin [10] but also numerous ...
Putting Process and Product Conceptions of Natural Selection and
... It might be thought that only a priori philosophical work could adjudicate the question of which class of definitions of selection and drift we should adopt. The argument would go something like this: If we’ve done our jobs as philosophers of biology correctly, the extensions of scientific concepts ...
... It might be thought that only a priori philosophical work could adjudicate the question of which class of definitions of selection and drift we should adopt. The argument would go something like this: If we’ve done our jobs as philosophers of biology correctly, the extensions of scientific concepts ...
File - Respiratory Therapy Files
... improve the ability to expectorate purulent secretions. Medications that increase airway water or the volume of airway secretions, including secretagogues that are meant to increase the hydration of luminal secretions (eg, hypertonic saline or mannitol) and abhesives that decrease the adhesivity o ...
... improve the ability to expectorate purulent secretions. Medications that increase airway water or the volume of airway secretions, including secretagogues that are meant to increase the hydration of luminal secretions (eg, hypertonic saline or mannitol) and abhesives that decrease the adhesivity o ...
Conformational changes in actin–myosin isoforms probed by Ni(II
... confirming the data obtained with the nucleotide-free myosin motor domain, and its relevance to actin binding, we have taken a solution-based approach to examine actin–myosin complexes through the use of Ni(II)Gly–Gly–His metallopeptides. Peptides of the general form Xaa–Xaa–His bind avidly to Cu(II ...
... confirming the data obtained with the nucleotide-free myosin motor domain, and its relevance to actin binding, we have taken a solution-based approach to examine actin–myosin complexes through the use of Ni(II)Gly–Gly–His metallopeptides. Peptides of the general form Xaa–Xaa–His bind avidly to Cu(II ...
Pseudouridine at position 55 in tRNA controls the contents of other
... that the 55 in archaeal tRNA is formed by Cbf5 (18– 20) and Pus10 (18,21), and that 55 in eukaryotic cytoplasmic and mitochondrial tRNA is formed by Pus4 (22). Although the truB genes are found in almost all eubacterial genomes (23,24), the gene has been experimentally identified in E. coli (17), B ...
... that the 55 in archaeal tRNA is formed by Cbf5 (18– 20) and Pus10 (18,21), and that 55 in eukaryotic cytoplasmic and mitochondrial tRNA is formed by Pus4 (22). Although the truB genes are found in almost all eubacterial genomes (23,24), the gene has been experimentally identified in E. coli (17), B ...
TIB_34 - Stored Product Insect and Engineering Research
... conditions for both sexes in Reps 1-3 (see Table 5 and 6). Additionally, disregarding the effect of the environment in which the selections were practiced, the crowded testing conditions produced a significantly greater mean in both sexes for Reps 1,3 and 4 (see Tables 5 and 7). These results imply ...
... conditions for both sexes in Reps 1-3 (see Table 5 and 6). Additionally, disregarding the effect of the environment in which the selections were practiced, the crowded testing conditions produced a significantly greater mean in both sexes for Reps 1,3 and 4 (see Tables 5 and 7). These results imply ...
Supplementary Figure Legends (doc 34K)
... Supplementary Figure S2. Flow chart for NF1 comprehensive mutation screening. Prescreening of large NF1 deletions by four intragenic microsatellites genotyping leads to a complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of ...
... Supplementary Figure S2. Flow chart for NF1 comprehensive mutation screening. Prescreening of large NF1 deletions by four intragenic microsatellites genotyping leads to a complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of ...
DNA Methylation Analysis
... multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or uracils (derived from unmethylated cytosine). This differentially converted DNA is interrogated ...
... multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or uracils (derived from unmethylated cytosine). This differentially converted DNA is interrogated ...
Lesson Overview - Enfield High School
... Plant and animal breeders often make use mutations. For example, when a complete set of chromosomes fails to separate during meiosis, the gametes that result may produce triploid (3N) or tetraploid (4N) organisms. The condition in which an organism has extra sets of chromosomes is called polyploidy. ...
... Plant and animal breeders often make use mutations. For example, when a complete set of chromosomes fails to separate during meiosis, the gametes that result may produce triploid (3N) or tetraploid (4N) organisms. The condition in which an organism has extra sets of chromosomes is called polyploidy. ...
PREIMPLANTATION GENETIC DIAGNOSIS
... Preimplantation testing of embryos is also used routinely in animal husbandry to produce animals of the preferred sex5. However, the clinical application of this type of technology, in an attempt to prevent transmission of genetic disease in humans, is still evolving. Measuring cytoplasmic enzyme ac ...
... Preimplantation testing of embryos is also used routinely in animal husbandry to produce animals of the preferred sex5. However, the clinical application of this type of technology, in an attempt to prevent transmission of genetic disease in humans, is still evolving. Measuring cytoplasmic enzyme ac ...
Sequence variation characteristics of D
... |.im. Time-lapse microcinematography of living cells, however, shows that mitochondria are remarkably mobile and plastic organelles, constantly changing their shape and even fijsing with one another and then separating again. As they move about in the cytoplasm, they often appear to be associated wi ...
... |.im. Time-lapse microcinematography of living cells, however, shows that mitochondria are remarkably mobile and plastic organelles, constantly changing their shape and even fijsing with one another and then separating again. As they move about in the cytoplasm, they often appear to be associated wi ...
Write on zinc fingers
... The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity. The structure of the zinc finger protein Zif268 bound to DNA described by Pavletich and Pabo in their 1991 publication has been key to much of this work and describes ...
... The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity. The structure of the zinc finger protein Zif268 bound to DNA described by Pavletich and Pabo in their 1991 publication has been key to much of this work and describes ...
Malate Dehydrogenases – Structure and Function
... structure of Escherichia coli MDH-citrate complex with pig cytoplasmic MDHNAD complex shows that the structures are essentially identical, although there is only about 20 % sequence identity (Hall et al. 1992). In general, MDHs are stable as dimers, which indicate the important connection of protein ...
... structure of Escherichia coli MDH-citrate complex with pig cytoplasmic MDHNAD complex shows that the structures are essentially identical, although there is only about 20 % sequence identity (Hall et al. 1992). In general, MDHs are stable as dimers, which indicate the important connection of protein ...
The trp Operon - aandersonbiology
... expression. When the puzzle is completed, it will have a rectangular shape. The center piece is the one with the DNA diagram on it. The blackline master to the term puzzle will serve as your answer key. The students are to signal you when they have completed their puzzle so that you can initial thei ...
... expression. When the puzzle is completed, it will have a rectangular shape. The center piece is the one with the DNA diagram on it. The blackline master to the term puzzle will serve as your answer key. The students are to signal you when they have completed their puzzle so that you can initial thei ...
Organic Molecules chapt03
... Some proteins contain more than one polypeptide chain. Each of these polypeptides has its own unique tertiary structure. ...
... Some proteins contain more than one polypeptide chain. Each of these polypeptides has its own unique tertiary structure. ...
Epigenetic Regulation ofbdnfGene Transcription in the
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
Identification of two amino acid residues which - Wiley-VCH
... a crystallized enzyme-substrate complex of the related enzyme chitobiase. The model contains a central hydrophilic region referred to as the “substrate cavity”. Figure 2 shows only this portion of the proposed structure. The three residues implicated in active site function cluster on the “left” sid ...
... a crystallized enzyme-substrate complex of the related enzyme chitobiase. The model contains a central hydrophilic region referred to as the “substrate cavity”. Figure 2 shows only this portion of the proposed structure. The three residues implicated in active site function cluster on the “left” sid ...
Nucleotide sequence and taxonomic value of the
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
Deoxyribozyme
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.