Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... (Seikagaku). For activity experiments, 2 µl of enzyme was placed in 248 µl of 50 mM Tris/HCl and 50 mM sodium acetate, pH 8.0, with 1 mg/ml substrate (0.25 mg/ml for hyaluronan) at 37 ◦C. Product formation was monitored as an increase in absorbance at 232 nm as a function of time in our semi-high-th ...
... (Seikagaku). For activity experiments, 2 µl of enzyme was placed in 248 µl of 50 mM Tris/HCl and 50 mM sodium acetate, pH 8.0, with 1 mg/ml substrate (0.25 mg/ml for hyaluronan) at 37 ◦C. Product formation was monitored as an increase in absorbance at 232 nm as a function of time in our semi-high-th ...
Single-stranded heteroduplex intermediates in l Red homologous
... mediated by the endogenous HR machinery [6-9]. Recombineering mainly refers to the engineering of recombinant DNA by HR [7,10-12]. Both gene targeting and recombineering usually involve the generation of a linear dsDNA copy of a segment of the chosen target DNA. A stretch of heterologous dsDNA, whic ...
... mediated by the endogenous HR machinery [6-9]. Recombineering mainly refers to the engineering of recombinant DNA by HR [7,10-12]. Both gene targeting and recombineering usually involve the generation of a linear dsDNA copy of a segment of the chosen target DNA. A stretch of heterologous dsDNA, whic ...
Comparison of the Detection Limits of the Culture and PCR Methods
... The prevalence levels of Campylobacter, Y. enterocolitica, and C. difficile are about 10.8%, 1.2%, and 21%, respectively, in stool samples of patients with gastroenteritis in developing countries (28-30). These rates are lower than those reported for developed countries (4, 31). Despite differences ...
... The prevalence levels of Campylobacter, Y. enterocolitica, and C. difficile are about 10.8%, 1.2%, and 21%, respectively, in stool samples of patients with gastroenteritis in developing countries (28-30). These rates are lower than those reported for developed countries (4, 31). Despite differences ...
icsi sperm selection by ha binding
... sperm morphology, is of limited predictive value. Different fertilization rates were assessed via IVF or ICSI even with sybling oocytes. No semen parameters are identified that would predict whether IVF or ICSI is more beneficial for a particular couple. ...
... sperm morphology, is of limited predictive value. Different fertilization rates were assessed via IVF or ICSI even with sybling oocytes. No semen parameters are identified that would predict whether IVF or ICSI is more beneficial for a particular couple. ...
Induced-fit of the peptidyl-transferase center of the
... transfer reaction (Johansson et al. 2008). This is consistent with the result that the induced-fit is not critical with Pm. The methyl tyrosine side-chain of this minimal substrate is very similar to that of phenylalanine, an aspect that is central to our analysis. Notwithstanding some uncertainties ...
... transfer reaction (Johansson et al. 2008). This is consistent with the result that the induced-fit is not critical with Pm. The methyl tyrosine side-chain of this minimal substrate is very similar to that of phenylalanine, an aspect that is central to our analysis. Notwithstanding some uncertainties ...
IntroS09
... small molecules that serve in some functional role in biological organisms. Many are vitamins or are derived from vitamins; a vitamin is defined as an organic molecule that is necessary for metabolism but cannot be synthesized by the organism. Thus the same compound may be a vitamin for one organism ...
... small molecules that serve in some functional role in biological organisms. Many are vitamins or are derived from vitamins; a vitamin is defined as an organic molecule that is necessary for metabolism but cannot be synthesized by the organism. Thus the same compound may be a vitamin for one organism ...
Prying Into Prions - Colorado Parks and Wildlife
... activities found in most high school biology textbooks that address the chemistry of life, DNA and the genetic code, and protein synthesis. It is the third module developed by the DOW to address the specific learning objectives of high school students. Materials are inquiry based, develop critical t ...
... activities found in most high school biology textbooks that address the chemistry of life, DNA and the genetic code, and protein synthesis. It is the third module developed by the DOW to address the specific learning objectives of high school students. Materials are inquiry based, develop critical t ...
A new defense of adaptationism
... The fundamental challenge, then, is to find some empirical test for general adaptive hypotheses.6 If there cannot be done, then how can the practice of giving adaptive explanations be a normal and legitimate part of empirical science? In other words, the thesis of adaptationism would seem to be fals ...
... The fundamental challenge, then, is to find some empirical test for general adaptive hypotheses.6 If there cannot be done, then how can the practice of giving adaptive explanations be a normal and legitimate part of empirical science? In other words, the thesis of adaptationism would seem to be fals ...
Thiele et al.: `Genome-scale reconstruction of E. coli`s transcriptional
... GidA has FAD bound (data were not shown but mentioned in paper that they analyzed it) [31]. Based on this evidence, this was assumed to be a monomer. From cmnm5s2U over nm5s2U to mnm5s2U is done by mnmC [32]. Described as a monomer based on the MW [33] and their comments. 78 molecules/genome equival ...
... GidA has FAD bound (data were not shown but mentioned in paper that they analyzed it) [31]. Based on this evidence, this was assumed to be a monomer. From cmnm5s2U over nm5s2U to mnm5s2U is done by mnmC [32]. Described as a monomer based on the MW [33] and their comments. 78 molecules/genome equival ...
Selection for TnlO Tet Repressor Binding to tet Operator
... and RICHMOND1975; KLECKNERet al. 1975). Expression of resistance is regulated very tightly at the level of transcription (BECK et al. 1982). The regulatory tet region contains the tet promoters aswellastwo operators O1 and 0 2 (BERTRANDet al. 1983) which are bound by Tet repressor preventing transcr ...
... and RICHMOND1975; KLECKNERet al. 1975). Expression of resistance is regulated very tightly at the level of transcription (BECK et al. 1982). The regulatory tet region contains the tet promoters aswellastwo operators O1 and 0 2 (BERTRANDet al. 1983) which are bound by Tet repressor preventing transcr ...
The aconitase of Escherichia cok purification of the
... used with the oligonucleotide probes. The citB probe was labelled by incorporating digoxigenin-1 1-dUTP into a complementary strand of M13mplO-KJ9 by primer extension (1.2 pmol universal primer per pg template) and the 456 bp PstI-EcoRI fragment was released and then purified by electrophoresis in 0 ...
... used with the oligonucleotide probes. The citB probe was labelled by incorporating digoxigenin-1 1-dUTP into a complementary strand of M13mplO-KJ9 by primer extension (1.2 pmol universal primer per pg template) and the 456 bp PstI-EcoRI fragment was released and then purified by electrophoresis in 0 ...
2004-009_-Draft-Anne..
... serological methods may facilitate a presumptive diagnosis of symptomatic plant material; however, an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in thi ...
... serological methods may facilitate a presumptive diagnosis of symptomatic plant material; however, an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in thi ...
document
... codon-anticodon interaction (regardless of which amino acid is attached to the tRNA) •Each tRNA is matched with its amino acid long before it reaches the ribosome. •The match is made by a collection of remarkable enzymes, the aminoacyl-tRNA synthetases. •These enzymes charge each tRNA with the prope ...
... codon-anticodon interaction (regardless of which amino acid is attached to the tRNA) •Each tRNA is matched with its amino acid long before it reaches the ribosome. •The match is made by a collection of remarkable enzymes, the aminoacyl-tRNA synthetases. •These enzymes charge each tRNA with the prope ...
Molecular evolution of the melanocortin 1-receptor - Funpec-RP
... and Ctenomyidae) were obtained from GenBank. These families included the three major currently recognized lineages: 1) “mouse-related clade” (Muridae + Cricetidae + Heteromyidae + Geomyidae), 2) “squirrel-related clade” (Sciuridae), and 3) Ctenohystrica (Ctenomyidae) (Table 1 and Figure 1) (Blanga-K ...
... and Ctenomyidae) were obtained from GenBank. These families included the three major currently recognized lineages: 1) “mouse-related clade” (Muridae + Cricetidae + Heteromyidae + Geomyidae), 2) “squirrel-related clade” (Sciuridae), and 3) Ctenohystrica (Ctenomyidae) (Table 1 and Figure 1) (Blanga-K ...
Lecture 1: Key Concepts in Stereoselective Synthesis
... different metals as catalysts for enantioselective Mukaiyama aldol addition. Diverse stereo outcomes can be obtained based on the metal and ligand used in the complex. Bidentate chelation leads to the formation of a square pyramidal complex and the re aldehyde enantioface is shielded -> High level o ...
... different metals as catalysts for enantioselective Mukaiyama aldol addition. Diverse stereo outcomes can be obtained based on the metal and ligand used in the complex. Bidentate chelation leads to the formation of a square pyramidal complex and the re aldehyde enantioface is shielded -> High level o ...
Histones - scientia.global
... complete or are rapidly degraded during the S phase if DNA replication is cut short. This type of regulation also requires specialised factors made specifically for these histone mRNAs. As mentioned above, since they have a unique 3′ end, histone mRNAs require a different set of factors for their sy ...
... complete or are rapidly degraded during the S phase if DNA replication is cut short. This type of regulation also requires specialised factors made specifically for these histone mRNAs. As mentioned above, since they have a unique 3′ end, histone mRNAs require a different set of factors for their sy ...
Catalytic and transport cycles of ABC exporters
... ers, other topologies ranging from five to ten α‑helices per TMD have been observed [13,14]. Thus the TMDs of importers and exporters cannot be easily interchanged in models of transport. It is best to consider the coupling mechanisms of these proteins in three distinct groups on the basis of their ...
... ers, other topologies ranging from five to ten α‑helices per TMD have been observed [13,14]. Thus the TMDs of importers and exporters cannot be easily interchanged in models of transport. It is best to consider the coupling mechanisms of these proteins in three distinct groups on the basis of their ...
Structure and function of the GINS complex, a key component of the
... initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting thes ...
... initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting thes ...
as a PDF - CiteSeerX
... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
Biology Final Exam Review Questions Answer Section SHORT
... A cell that lacked cyclins would probably not undergo mitotic division, and then it would continue to grow, have DNA overload, and exchange materials inefficiently until it dies. PTS: 1 DIF: L3 REF: p. 286 OBJ: 10.3.1 Describe how the cell cycle is regulated. STA: CA.BIO.1.h BLM: evaluation 10. ANS: ...
... A cell that lacked cyclins would probably not undergo mitotic division, and then it would continue to grow, have DNA overload, and exchange materials inefficiently until it dies. PTS: 1 DIF: L3 REF: p. 286 OBJ: 10.3.1 Describe how the cell cycle is regulated. STA: CA.BIO.1.h BLM: evaluation 10. ANS: ...
SENSOR NETWORKS - Accueil | www.eurecom.fr
... communications in the Terahertz Band (0.1-10 THz) – Uniqueness of the Terahertz band: * Terahertz channel is seriously affected by the presence of different molecules present in the medium * High molecular absorption attenuates the travelling wave and introduces noise into the channel IFA’2010 ...
... communications in the Terahertz Band (0.1-10 THz) – Uniqueness of the Terahertz band: * Terahertz channel is seriously affected by the presence of different molecules present in the medium * High molecular absorption attenuates the travelling wave and introduces noise into the channel IFA’2010 ...
Answers set 7
... In most organisms, fatty acid synthase is a closely associated complex of seven catalytic centres surrounding acyl carrier protein (ACP) which carries a long pantetheine arm terminating in the HS- group that bonds to the substrate. In bacteria, the complex consists of independent polypeptides held t ...
... In most organisms, fatty acid synthase is a closely associated complex of seven catalytic centres surrounding acyl carrier protein (ACP) which carries a long pantetheine arm terminating in the HS- group that bonds to the substrate. In bacteria, the complex consists of independent polypeptides held t ...
Design principles for oxygen-reduction activity on perovskite oxide
... riven by growing concerns about global warming and the depletion of petroleum resources, developing renewable energy production and storage technologies represents one of the major scientific challenges of the twenty-first century. A critical element in the pursuit of this quest is the discovery of ef ...
... riven by growing concerns about global warming and the depletion of petroleum resources, developing renewable energy production and storage technologies represents one of the major scientific challenges of the twenty-first century. A critical element in the pursuit of this quest is the discovery of ef ...
Deoxyribozyme
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.