Chromatin Condensing Functions of the Linker Histone C
... extensively in recent years (see ref 20 and references therein). Intrinsically disordered protein domains mostly or completely lack classical secondary structure but undergo disorder to order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has be ...
... extensively in recent years (see ref 20 and references therein). Intrinsically disordered protein domains mostly or completely lack classical secondary structure but undergo disorder to order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has be ...
PDF - Biochemical Society Transactions
... whilst the latter becomes aromatized, and hence inert to cleavage, whilst bound to the pyridoxal. ...
... whilst the latter becomes aromatized, and hence inert to cleavage, whilst bound to the pyridoxal. ...
Evolutionary History of Free-Swimming and
... several authors, including Darwin (1871) and, more recently, Tokioka (1971), Jollie (1973), and Jefferies (1986). In contrast, Garstang (1928) proposed that the ancestral chordates had sessile adults and that the tadpole-like body plan evolved in the larval stage of these ancestors. Cephalochordates ...
... several authors, including Darwin (1871) and, more recently, Tokioka (1971), Jollie (1973), and Jefferies (1986). In contrast, Garstang (1928) proposed that the ancestral chordates had sessile adults and that the tadpole-like body plan evolved in the larval stage of these ancestors. Cephalochordates ...
Multiple Manner Transposons in Flatworms and Hydras Are Related
... in Table 1. Genomic DNA was extracted from parts of specimens of large organisms such as the anemones, individual animals of small species such as the sea walnut, or from multiple Individuals such as the hydra species using denaturation of proteins with SDS, precipitation of proteins with high salt, ...
... in Table 1. Genomic DNA was extracted from parts of specimens of large organisms such as the anemones, individual animals of small species such as the sea walnut, or from multiple Individuals such as the hydra species using denaturation of proteins with SDS, precipitation of proteins with high salt, ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... About 0.2/~g of protein from isolated gap junctions was separated on a 15% polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane (Millipore Continental Water Systems, Bedford, MA). The blots were blocked for 1.5 h at 37"C in TBS (0.01 M Tris, pH 7.4, 0.9% NaCI) containing 3 % ...
... About 0.2/~g of protein from isolated gap junctions was separated on a 15% polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane (Millipore Continental Water Systems, Bedford, MA). The blots were blocked for 1.5 h at 37"C in TBS (0.01 M Tris, pH 7.4, 0.9% NaCI) containing 3 % ...
Sexual selection can constrain sympatric speciation
... deviate. This initial condition is implausible when selection is initially stabilizing, as we discussed in § 3. We chose this initial condition to maximize the potential for sympatric speciation, so the results are conservative in the sense that we expect sympatric speciation to be even less likely ...
... deviate. This initial condition is implausible when selection is initially stabilizing, as we discussed in § 3. We chose this initial condition to maximize the potential for sympatric speciation, so the results are conservative in the sense that we expect sympatric speciation to be even less likely ...
Pet_SUMO manual
... proteins; however, unlike ubiquitin modification, SUMO modification leads to changes in protein function and activity rather than protein degradation. Studies at Invitrogen have shown that fusion of a heterologous protein to SUMO can lead to increased expression levels as well as enhanced solubility ...
... proteins; however, unlike ubiquitin modification, SUMO modification leads to changes in protein function and activity rather than protein degradation. Studies at Invitrogen have shown that fusion of a heterologous protein to SUMO can lead to increased expression levels as well as enhanced solubility ...
A New Sequencing Primer and Workflow Increase 5` Resolution and
... quality readable bases by base 25 after the sequencing primer while the new primers produced high quality bases by base 5, and by base 1 in many cases. Because of improved resolution, basecalling accuracy was increased. This simplified process without a separate PCR clean-up step reduced the overall ...
... quality readable bases by base 25 after the sequencing primer while the new primers produced high quality bases by base 5, and by base 1 in many cases. Because of improved resolution, basecalling accuracy was increased. This simplified process without a separate PCR clean-up step reduced the overall ...
PPT
... frameshift site between ORF1 (93 AAs) and ORF 2 (92 AAs) in S. cerevisiae and also many other yeast species. The frameshift site has the slippery sequence 5´-CUU AGU U-3´. AGU is encoded by a low abundance tRNA (sometimes referred to as a “hungry codon”), which frequently induces a ribosomal pau ...
... frameshift site between ORF1 (93 AAs) and ORF 2 (92 AAs) in S. cerevisiae and also many other yeast species. The frameshift site has the slippery sequence 5´-CUU AGU U-3´. AGU is encoded by a low abundance tRNA (sometimes referred to as a “hungry codon”), which frequently induces a ribosomal pau ...
Mutating the second glutamate in the amidase active site
... in a different manner than it does in the wildtype enzyme. The result is that the double bond of the acrylamide rather than the amide carbonyl carbon is adjacent to the active site cysteine. This demonstrates the role of the hydrogen bond between E142 Oε2 and the substrate amino group in positioning ...
... in a different manner than it does in the wildtype enzyme. The result is that the double bond of the acrylamide rather than the amide carbonyl carbon is adjacent to the active site cysteine. This demonstrates the role of the hydrogen bond between E142 Oε2 and the substrate amino group in positioning ...
CHAPTER 16 - CITRIC ACID CYCLE Introduction:
... activated by the high-energy indicator, ATP). Phosphorylation of E1 results in its inactivation. Note that insulin, which we have previously seen to have antagonistic effects with respect to glucagon and epinephrine, and which terminates glycogen breakdown and initiates glycogen synthesis, reverses ...
... activated by the high-energy indicator, ATP). Phosphorylation of E1 results in its inactivation. Note that insulin, which we have previously seen to have antagonistic effects with respect to glucagon and epinephrine, and which terminates glycogen breakdown and initiates glycogen synthesis, reverses ...
Introduction - Stellenbosch University
... 4. name - Defines the name of the BED line. This label is displayed to the left of the BED line in the Genome Browser window when the track is open to full display mode or directly to the left of the item in pack mode. 5. score - A score between 0 and 1000. 6. strand - Defines the strand. Either "." ...
... 4. name - Defines the name of the BED line. This label is displayed to the left of the BED line in the Genome Browser window when the track is open to full display mode or directly to the left of the item in pack mode. 5. score - A score between 0 and 1000. 6. strand - Defines the strand. Either "." ...
On the explanatory roles of natural selection
... so explain why particular individuals have them (1995b). Replies to the “positive” position – that selection is part of the explanation for why individuals have the traits they do – usually draw upon the distinction between variational and developmental explanations. Due to certain explanatory or me ...
... so explain why particular individuals have them (1995b). Replies to the “positive” position – that selection is part of the explanation for why individuals have the traits they do – usually draw upon the distinction between variational and developmental explanations. Due to certain explanatory or me ...
Alu - Environmental
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
A Physical Gene Map of the Bacteriophage P22 Late
... chromosome fragments. While restriction enzyme cleavage generates specific fragments which can be separated by gel electrophoresis, preparations of such fragments are often contaminated by heterogeneoussized pieces derived from permuted chromosome ends. In addition, identification of the genes prese ...
... chromosome fragments. While restriction enzyme cleavage generates specific fragments which can be separated by gel electrophoresis, preparations of such fragments are often contaminated by heterogeneoussized pieces derived from permuted chromosome ends. In addition, identification of the genes prese ...
Biological Inorganic Chemistry
... Printer & Binder: Maple-Vail Book Manufacturing Group This book is printed on acid-free paper. Copyright 6 2007 by University Science Books ISBN 10: 1-891389-43-2 ISBN 13: 978-1-891389-43-6 Reproduction or translation of any part of this work beyond that permitted by Section 107 or 108 of the 1976 U ...
... Printer & Binder: Maple-Vail Book Manufacturing Group This book is printed on acid-free paper. Copyright 6 2007 by University Science Books ISBN 10: 1-891389-43-2 ISBN 13: 978-1-891389-43-6 Reproduction or translation of any part of this work beyond that permitted by Section 107 or 108 of the 1976 U ...
MIDWESTERN ASSOCIATION OF FORENSIC SCIENTISTS, INC
... opportunity to examine a variety of dusts, including those which they themselves bring with them. The manner in which a dust sample has been collected has an important bearing on the techniques used to examine it. Dust may be received as a loose powder, from vacuuming, as scrapings, adhering to adhe ...
... opportunity to examine a variety of dusts, including those which they themselves bring with them. The manner in which a dust sample has been collected has an important bearing on the techniques used to examine it. Dust may be received as a loose powder, from vacuuming, as scrapings, adhering to adhe ...
Organic Chemistry/Fourth Edition: e-Text
... amino acids, but analogous principles apply. Because they incorporate different numbers of amino acids and because their side chains are different, two peptides will have slightly different acid–base properties and slightly different net charges at a particular pH. Thus, their mobilities in an elect ...
... amino acids, but analogous principles apply. Because they incorporate different numbers of amino acids and because their side chains are different, two peptides will have slightly different acid–base properties and slightly different net charges at a particular pH. Thus, their mobilities in an elect ...
Trichothiodystrophy: Update on the sulfur
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
Scholarly Interest Report
... The discipline of bioenergetics attempts to characterize the biochemical processes whereby the chemical free energy that originates with our diet is made available to living organisms. In eucaryotic systems the relevant processes are catalyzed by enzyme complexes present in the inner membrane of the ...
... The discipline of bioenergetics attempts to characterize the biochemical processes whereby the chemical free energy that originates with our diet is made available to living organisms. In eucaryotic systems the relevant processes are catalyzed by enzyme complexes present in the inner membrane of the ...
Isoenzymes in Clinical Diagnosis
... Two groups of phosphatase were originally detected by their different pH optima and called "acid" and "alkaline" phosphatase. Further "isoenzymes" of phosphatase were differentiated by susceptibility to inhibition by tartrate and other chemicals.'6 Different pepsins were separated by differences in ...
... Two groups of phosphatase were originally detected by their different pH optima and called "acid" and "alkaline" phosphatase. Further "isoenzymes" of phosphatase were differentiated by susceptibility to inhibition by tartrate and other chemicals.'6 Different pepsins were separated by differences in ...
Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.