1 - UCSB C.L.A.S.
... isomerase indicates that one of the amino acid side chains at the active site has a pKa of 9.3. Identify the amino acid side chain. With a pKa of 9.3 we look at AAs with side chains that have similar pKas: Lys (10.79), Cys (8.35), Tyr (10.07) Since that is the pKa of the group on the descending leg ...
... isomerase indicates that one of the amino acid side chains at the active site has a pKa of 9.3. Identify the amino acid side chain. With a pKa of 9.3 we look at AAs with side chains that have similar pKas: Lys (10.79), Cys (8.35), Tyr (10.07) Since that is the pKa of the group on the descending leg ...
Metabolism of Macromolecules in Bacteria Treated
... reversible phase, which was characterized by a long lag in colony formation. Very short incubation with single virginiamycin components and their combination suddenly and completely blocked protein synthesis, whereas the rate of incorporation of labelled bases and nucleosides into polynucleotides wa ...
... reversible phase, which was characterized by a long lag in colony formation. Very short incubation with single virginiamycin components and their combination suddenly and completely blocked protein synthesis, whereas the rate of incorporation of labelled bases and nucleosides into polynucleotides wa ...
Isoprenoid metabolism: cholesterol and the others
... mitochondrial matrix while steroid synthesis takes place in the cytosol. The next step is both important and somewhat unusual. It is the rate limiting step of the whole pathway and as such it determines how much cholesterol (and other isoprenoids) we can make per unit time. hmg-CoA is reduced by the ...
... mitochondrial matrix while steroid synthesis takes place in the cytosol. The next step is both important and somewhat unusual. It is the rate limiting step of the whole pathway and as such it determines how much cholesterol (and other isoprenoids) we can make per unit time. hmg-CoA is reduced by the ...
Functional Domains within Fusion Proteins: Prospectives for
... Pritsker et al., 1999), as well as from studies with synthetic fusion peptides (Lear and DeGrado, 1987; Slepushkin et al., 1990; Burger et al., 1991; Clague et al., 1991; Martin et al., 1991; Yeagle et al., 1991; Epand et al., 1994; Nieva et al., 1994; Rapaport & Shai, 1994; Gray et al., 1996; Klige ...
... Pritsker et al., 1999), as well as from studies with synthetic fusion peptides (Lear and DeGrado, 1987; Slepushkin et al., 1990; Burger et al., 1991; Clague et al., 1991; Martin et al., 1991; Yeagle et al., 1991; Epand et al., 1994; Nieva et al., 1994; Rapaport & Shai, 1994; Gray et al., 1996; Klige ...
Protein targeting, translocation and Escherichia coli Proteomic analysis of substrate-pathway relationships
... envelope of chloroplasts. β-barrel membrane proteins are composed of an even number of anti-parallel β-strands arranged in a barrel like structure. The amide and carbonyl groups of the polypeptide backbone are stabilized by hydrogen bonds between neighboring strands. Amino acid side chains of mixed ...
... envelope of chloroplasts. β-barrel membrane proteins are composed of an even number of anti-parallel β-strands arranged in a barrel like structure. The amide and carbonyl groups of the polypeptide backbone are stabilized by hydrogen bonds between neighboring strands. Amino acid side chains of mixed ...
Examination #2 1) Which of the following is not one of the four major
... Skill: Knowledge 40) The R group or side chain of the amino acid serine is –CH2-OH. The R group or side chain of the amino acid alanine is –CH3. Where would you expect to find these amino acids in a globular protein in aqueous solution? A) Serine would be in the interior, and alanine would be on the ...
... Skill: Knowledge 40) The R group or side chain of the amino acid serine is –CH2-OH. The R group or side chain of the amino acid alanine is –CH3. Where would you expect to find these amino acids in a globular protein in aqueous solution? A) Serine would be in the interior, and alanine would be on the ...
STEROIDS, BILE ACIDS, STEROID HORMONES
... role in calcium and phosphate metabolism. D Vitamins are generated from the provitamins ergosterol and 7-dehydrocholesterol by the action of sunlight. Ergosterol occurs in plants and 7-dehydrocholesterol in animals. UV light from sunlight cleaves the B ring of both compounds. Ergocalciferol (vit ...
... role in calcium and phosphate metabolism. D Vitamins are generated from the provitamins ergosterol and 7-dehydrocholesterol by the action of sunlight. Ergosterol occurs in plants and 7-dehydrocholesterol in animals. UV light from sunlight cleaves the B ring of both compounds. Ergocalciferol (vit ...
Page 1
... Cui et al., "The Permissive Effect of Zinc Deficiency on Uroguanylin and Inducible Nitric Oxide Synthase Gene Upregulation in Rat Intestine Induced by Interleukin la is Rapidly Reversed by Zinc Repletion", J. Nutri.,133(l): 51-56 (2003). Currie et al., "Guanylin: an endogenous activator of intestina ...
... Cui et al., "The Permissive Effect of Zinc Deficiency on Uroguanylin and Inducible Nitric Oxide Synthase Gene Upregulation in Rat Intestine Induced by Interleukin la is Rapidly Reversed by Zinc Repletion", J. Nutri.,133(l): 51-56 (2003). Currie et al., "Guanylin: an endogenous activator of intestina ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
Biomarker discovery for psychiatric disorders
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
148 - Blue Ridge Institute for Medical Research
... kinases contain a K/E/D/D signature sequence that plays important structural and catalytic roles. Protein kinases contain hydrophobic catalytic and regulatory spines and collateral shell residues that are required to assemble the active enzyme. There are two general kinds of conformational changes a ...
... kinases contain a K/E/D/D signature sequence that plays important structural and catalytic roles. Protein kinases contain hydrophobic catalytic and regulatory spines and collateral shell residues that are required to assemble the active enzyme. There are two general kinds of conformational changes a ...
Protein Acetylation as an Integral Part of Metabolism in Cancer
... cell cycle genes and Sir2 regulates mostly amino-acid biosynthesis [32]. HDAC1 in Drosophila appears to be the major “housekeeping” enzyme regulating diverse sets of genes most significantly those involved in cell proliferation and mitochondrial energy metabolism [12]. From studies on model systems ...
... cell cycle genes and Sir2 regulates mostly amino-acid biosynthesis [32]. HDAC1 in Drosophila appears to be the major “housekeeping” enzyme regulating diverse sets of genes most significantly those involved in cell proliferation and mitochondrial energy metabolism [12]. From studies on model systems ...
Design of Tight-Binding Human Immunodeficiency
... disease to mankind. Until this day, human immune deficiency virus, HIV, has caused the death of many humans and infected patients have little chance to become healthy. In the beginning, people become exposed to HIV mainly by unsafe sex with HIV positive person of the same sex. But the situation has ...
... disease to mankind. Until this day, human immune deficiency virus, HIV, has caused the death of many humans and infected patients have little chance to become healthy. In the beginning, people become exposed to HIV mainly by unsafe sex with HIV positive person of the same sex. But the situation has ...
Supplementary Text and Figures
... way that it encompasses the product as it exists in nature. For example, if a claim defines protein X, bacteria X or gene X, where protein X, bacteria X or gene X are naturally occuring, then the claim would be considered to ...
... way that it encompasses the product as it exists in nature. For example, if a claim defines protein X, bacteria X or gene X, where protein X, bacteria X or gene X are naturally occuring, then the claim would be considered to ...
fermentation?
... Human beings are known to have made fermented foods since Neolithic times. The earliest types were beer, wine, and leavened bread (made primarily by yeasts) and cheeses (made by bacteria and molds). These were soon followed by East Asian fermented foods, yogurt and other fermented milk products, pic ...
... Human beings are known to have made fermented foods since Neolithic times. The earliest types were beer, wine, and leavened bread (made primarily by yeasts) and cheeses (made by bacteria and molds). These were soon followed by East Asian fermented foods, yogurt and other fermented milk products, pic ...
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a
... polioviruses sequenced to date. Interestingly, the single nucleotide substitution in the 3' noncoding region of strain 23127 destroys the second of these stop codons, indicating that this is not required for the viability of the virus. In addition to the 5' non-coding region, the other seemingly ano ...
... polioviruses sequenced to date. Interestingly, the single nucleotide substitution in the 3' noncoding region of strain 23127 destroys the second of these stop codons, indicating that this is not required for the viability of the virus. In addition to the 5' non-coding region, the other seemingly ano ...
PowerPoint 演示文稿
... mRNA has the same function in all cells, but there are important differences in the details of the synthesis and structure between prokaryotic and eukaryotic mRNA. ...
... mRNA has the same function in all cells, but there are important differences in the details of the synthesis and structure between prokaryotic and eukaryotic mRNA. ...
Distinct C-terminal Amino Acid Sequence Motifs Serve
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
ppt - eweb.furman.edu
... 2. Polymerization of monomers into polymers (proteins, RNA, sugars, fats, etc.) - strong evidence; Fox and Cairns-Smith experiments. 3. Formation of membranes - strong evidence; behavior of phospholipids in solution. 4. Evolution of metabolic systems - reasonable hypotheses, and genetic similarity i ...
... 2. Polymerization of monomers into polymers (proteins, RNA, sugars, fats, etc.) - strong evidence; Fox and Cairns-Smith experiments. 3. Formation of membranes - strong evidence; behavior of phospholipids in solution. 4. Evolution of metabolic systems - reasonable hypotheses, and genetic similarity i ...
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a
... polioviruses sequenced to date. Interestingly, the single nucleotide substitution in the 3' noncoding region of strain 23127 destroys the second of these stop codons, indicating that this is not required for the viability of the virus. In addition to the 5' non-coding region, the other seemingly ano ...
... polioviruses sequenced to date. Interestingly, the single nucleotide substitution in the 3' noncoding region of strain 23127 destroys the second of these stop codons, indicating that this is not required for the viability of the virus. In addition to the 5' non-coding region, the other seemingly ano ...
allosteric inhibition
... • Vmax is unchanged: At high levels of substrate all of the inhibitor is displaced by substrate. • KM is increased: Higher substrate concentrations are required to reach the maximal velocity. ...
... • Vmax is unchanged: At high levels of substrate all of the inhibitor is displaced by substrate. • KM is increased: Higher substrate concentrations are required to reach the maximal velocity. ...
pdf file - 366KB
... represented by hydrogen bond may be assessed and inferred in terms of electrostatic character and steric orientation. Figure 4a shows the bent conformation tetrahydrofolic acid with its PABA residue above the pteridine ring, allowing tight enzyme and nucleotide binding. In this thymidylate synthase- ...
... represented by hydrogen bond may be assessed and inferred in terms of electrostatic character and steric orientation. Figure 4a shows the bent conformation tetrahydrofolic acid with its PABA residue above the pteridine ring, allowing tight enzyme and nucleotide binding. In this thymidylate synthase- ...
NUCLEOTIDE and PROTEIN databases
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.