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European Journal of Biochemistry
European Journal of Biochemistry

... incorporation of phospholipid into protein d/lipopolysaccharide complexes, indicating that phospholipids are part of the environment of the phage K3 receptor in cells of this mutant, but not in wildtype cells ...
Hydrolyzed Source Proteins as Used in Cosmetics
Hydrolyzed Source Proteins as Used in Cosmetics

... The definitions of these ingredients are presented in Table 1. The ingredients in this group are interrelated because they each are prepared from proteins by partial hydrolysis to yield cosmetically acceptable raw materials. The definitions exclude the complete hydrolysis products of proteins, the a ...
Enzymatic Activities for the Synthesis of Chlorophyll in Pigment
Enzymatic Activities for the Synthesis of Chlorophyll in Pigment

... Quantitation of the rate of porphyrin synthesis—The rate of porphyrin synthesis was determined by a modified version of the procedure of Pardo et al. (1980). Tissues were homogenized in the same buffer as that used for assays of PBG synthase, and extracts were centrifuged at 10,000xg for 15 min at 4 ...
post- translational modifications
post- translational modifications

... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
clinical biochemistry
clinical biochemistry

... test; Renal function test; Liver function test ...
Pain-Free Biochemistry. An Essential Guide for the Health Sciences Brochure
Pain-Free Biochemistry. An Essential Guide for the Health Sciences Brochure

... Pain-Free Biochemistry. An Essential Guide for the Health Sciences Description: ...
Properties of ATP - BioWiki
Properties of ATP - BioWiki

... Likewise we make about the same amount from the turnover products. When energy is needed, carbohydrates and lipids are oxidized and ATP is produced, which can then be immediately used for motility, biosynthesis, etc. It is very important to realize that although ATP is converted to ADP in a thermody ...
Purification and Partial Characterization of an Acid
Purification and Partial Characterization of an Acid

... SDS-gel electrophoresis of the purified acid phosphatase revealed two protein bands, a slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sepha ...
AP Biology Cell Unit Exam - Speedway High School
AP Biology Cell Unit Exam - Speedway High School

... originated before the last universal common ancestor of life on Earth? A) Glycolysis is found in all eukaryotic cells. B) Ancient prokaryotic cells, the most primitive of cells, made extensive use of glycolysis long before oxygen was present in Earth's atmosphere. C) Glycolysis is widespread and is ...
FREE Sample Here
FREE Sample Here

... 53) Lipophobic molecules that are to be released by cells are stored in membrane-bound structures called ________. A) secretory vesicles B) inclusions C) the Golgi apparatus D) excretory vesicles E) the endoplasmic reticulum Answer: A Diff: 4 Page Ref: 32 54) Continuous with the outer portion of the ...
Gq-dependent signalling by the lysophosphatidic acid receptor
Gq-dependent signalling by the lysophosphatidic acid receptor

... and Rho kinase (Rho-associated kinase) activities: stimulation of all three enzymes was inhibited by expression of the Gαq , but not the Gαi , minigene. Initial contraction and MLC20 (20 kDa regulatory light chain of myosin II) phosphorylation induced by LPA were abolished by inhibitors of PLC (phos ...
Microbial degradation of chloroaromatics Mars, Astrid E.
Microbial degradation of chloroaromatics Mars, Astrid E.

... Pseudomonas putida GJ31 is able to simultaneously grow on toluene and chlorobenzene. When cultures of this strain were inhibited with 3-fluorocatechol while growing on toluene or chlorobenzene, 3-methylcatechol or 3-chlorocatechol, respectively, accumulated in the medium. To establish the catabolic ...
Purification and Partial Characterization of an Acid
Purification and Partial Characterization of an Acid

... SDS-gel electrophoresis of the purified acid phosphatase revealed two protein bands, a slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sepha ...
Standard PDF - Wiley Online Library
Standard PDF - Wiley Online Library

... rapidly become a powerful tool in studies of food web architecture, resource use, and biogeochemical cycling. However, applications to avian ecology have been limited because no controlled studies have examined the patterns in AA isotope fractionation in birds. We conducted a controlled CSIA feeding ...
A Diurnal Component to the Variation in Sieve Tube Amino Acid
A Diurnal Component to the Variation in Sieve Tube Amino Acid

AMINO ACID SEQUENCES AND EVOLUTIONARY RELATIONSHIPS
AMINO ACID SEQUENCES AND EVOLUTIONARY RELATIONSHIPS

... AMINO ACID SEQUENCES AND EVOLUTIONARY RELATIONSHIPS BIO H LAB DR WEINER Background Homologous structures, those structures believed to have a common origin but not necessarily a common function, provide some of the most significant evidence supporting the theory of evolution. For example, the foreli ...
Ser/Thr-specific protein phosphatases are
Ser/Thr-specific protein phosphatases are

... U5 snRNPs. The data indicate that reversible protein phosphorylation may play an Important role in regulating the pre-mRNA splicing mechanism. INTRODUCTION Reversible phosphorylation of proteins is one of the major mechanisms used to regulate cellular processes, including metabolism, initiation of m ...
On the Uniqueness of the Standard Genetic Code
On the Uniqueness of the Standard Genetic Code

... DNA replication, protein manufacture and biochemical pathways. Code stability since its formation on the early Earth has contributed to preserving evidence of the transition from an RNA World to a protein-dependent world. The transfer RNA (tRNA) is perhaps the most important molecule in the origin a ...
Cholesterol And Sterol Metabolism
Cholesterol And Sterol Metabolism

... Glucagon stimulates adenyl cyclase producing cAMP cAMP activates protein kinase A Inactivates HMG-CoA reductase ...
acyl-CoA
acyl-CoA

Metabolic flux profiling of recombinant protein secreting Pichia
Metabolic flux profiling of recombinant protein secreting Pichia

Developement of strategies for the isotopic labeling of methyl
Developement of strategies for the isotopic labeling of methyl

... on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using this protocol, assignment of the methyl groups signals is mandatory. Two strategies were then proposed. The first is suitable for systems whose molecular weight does not exceed 100 kDa. It relies o ...
Blood Sugar is Stable
Blood Sugar is Stable

... of cortisol is that, while synthesis of most proteins decreases with increased cortisol, synthesis of enzymes involved in gluconeogenesis increases in the liver. We get a situation where amino acids are released as substrates for glucose formation and increased gluconeogenetic enzyme levels to do th ...
New lysosomal acid lipase gene mutants explain the phenotype of
New lysosomal acid lipase gene mutants explain the phenotype of

... esterification (1). The importance of LAL in the regulation of intracellular cholesterol flux is further supported by the fact that an altered LAL function has been implicated in the development of atherosclerosis in the population at large (2, 3). LAL is a 378-amino acid protein containing a 27-ami ...
Diabetes Mellitus Overview and Treatments
Diabetes Mellitus Overview and Treatments

... The bulk of the pancreas is an exocrine gland secreting pancreatic fluid into the duodenum after a meal. Inside the pancreas are millions of clusters of cells called islets of Langerhans. The islets are endocrine tissue containing four types of cells. In order of abundance, they are: beta cells, wh ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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