Molecular cloning, characterization and gene expression of an
... Keywords: Catalase Macrobrachium rosenbergii IHHNV Gene expression Protein characterization ...
... Keywords: Catalase Macrobrachium rosenbergii IHHNV Gene expression Protein characterization ...
Gene expression profiling of mice with genetically modified muscle
... different GLUTs (glucose transporters) to mediate glucose entry into the cell, as well as different forms of hexokinase/glucokinase for the phosphorylation of glucose. There are two genes, GYS1 and GYS2, encoding glycogen synthase in both mouse and human genomes. To date, GYS2 is only known to be ex ...
... different GLUTs (glucose transporters) to mediate glucose entry into the cell, as well as different forms of hexokinase/glucokinase for the phosphorylation of glucose. There are two genes, GYS1 and GYS2, encoding glycogen synthase in both mouse and human genomes. To date, GYS2 is only known to be ex ...
Identification of two amino acid residues which - Wiley-VCH
... present in Hex A and Hex S, but not in Hex B, hydrolyzes negatively charged substrates [8, 9, 3, 10]. Early studies of the catalytic mechanism of hexosaminidase enzymes focused on the identification of amino acid residues comprising the enzyme active sites. Three Hex A residues, R178 [11], D258 [12] ...
... present in Hex A and Hex S, but not in Hex B, hydrolyzes negatively charged substrates [8, 9, 3, 10]. Early studies of the catalytic mechanism of hexosaminidase enzymes focused on the identification of amino acid residues comprising the enzyme active sites. Three Hex A residues, R178 [11], D258 [12] ...
Quantitative Proteomics of Transporter Expression in Brain Capillary
... absence of Bcrp in the Bcrp1(⫺/⫺) mice did not significantly influence the brain distribution of drugs that were shown to be good Bcrp substrates in vitro (Kodaira et al., 2010; Agarwal et al., 2011b). These findings have led to several questions about the predictive validity of the transgenic mouse ...
... absence of Bcrp in the Bcrp1(⫺/⫺) mice did not significantly influence the brain distribution of drugs that were shown to be good Bcrp substrates in vitro (Kodaira et al., 2010; Agarwal et al., 2011b). These findings have led to several questions about the predictive validity of the transgenic mouse ...
Molecular Immunology Circular Dichroism reveals evidence of coupling between immunoglobulin
... Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determin ...
... Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determin ...
Syllabus (Biotech) - Karunya University
... 5. Estimation of alkalinity in water sample 6. Estimation of dissolved oxygen 7. Estimation of Iron in water sample by spectrophotometry 8. pH measuremnts for acid – alkali titration 9. Conductometric estimation of an acid 10. Potentiometric estimation of Fe2+ Ions 11. Determination of single electr ...
... 5. Estimation of alkalinity in water sample 6. Estimation of dissolved oxygen 7. Estimation of Iron in water sample by spectrophotometry 8. pH measuremnts for acid – alkali titration 9. Conductometric estimation of an acid 10. Potentiometric estimation of Fe2+ Ions 11. Determination of single electr ...
ppt - Chair of Computational Biology
... Conclusions about large-scale structure In a cell or microorganism, the processes that generate mass, energy, information transfer and cell-fate specification are seamlessly integrated through a complex network of cellular constituents and reactions. A systematic comparative mathematical analysis o ...
... Conclusions about large-scale structure In a cell or microorganism, the processes that generate mass, energy, information transfer and cell-fate specification are seamlessly integrated through a complex network of cellular constituents and reactions. A systematic comparative mathematical analysis o ...
Introduction to Carbohydrates
... • Therefore, only the conjugated forms—that is, the bile salts—are found in the bile. • Individuals with genetic deficiencies in the conversion of cholesterol to bile acids are treated with exogenously supplied chenodeoxycholic acid. [Bile salts provide the only significant mechanism for cholesterol ...
... • Therefore, only the conjugated forms—that is, the bile salts—are found in the bile. • Individuals with genetic deficiencies in the conversion of cholesterol to bile acids are treated with exogenously supplied chenodeoxycholic acid. [Bile salts provide the only significant mechanism for cholesterol ...
Nutritional Control of Growth and Development in Yeast
... nutritional depletion by engaging one of a number of alternative developmental programs depending on the particular nutritional circumstances. These programs can range from rapid mitotic growth in rich media, to filamentous growth allowing foraging under limiting nutrient conditions, to various disti ...
... nutritional depletion by engaging one of a number of alternative developmental programs depending on the particular nutritional circumstances. These programs can range from rapid mitotic growth in rich media, to filamentous growth allowing foraging under limiting nutrient conditions, to various disti ...
Bitter flavour in dairy products. II. A review of bitter peptides
... strains were due to the inability of bitter strains to hydrolyse the bitter peptides produced by rennet. An experiment conducted by Emmons et al (1962a) led them to suggest that strains which produce bitter cheese are deficient in proteolytic enzymes that normally decomposé the bittertasting peptide ...
... strains were due to the inability of bitter strains to hydrolyse the bitter peptides produced by rennet. An experiment conducted by Emmons et al (1962a) led them to suggest that strains which produce bitter cheese are deficient in proteolytic enzymes that normally decomposé the bittertasting peptide ...
Biochemistry –Second year, Coll
... The next note is who the human body deals with the products of the metabolized TG; the fatty acids and glycerol. The fatty acids formed in adipocytes are either reuesd again in adipocytes(intraadipocytes) for the synthesis of new molecules of TG(lipogenesis) as the two pathways of liogenesis and lip ...
... The next note is who the human body deals with the products of the metabolized TG; the fatty acids and glycerol. The fatty acids formed in adipocytes are either reuesd again in adipocytes(intraadipocytes) for the synthesis of new molecules of TG(lipogenesis) as the two pathways of liogenesis and lip ...
A family of diatom-like silicon transporters in the siliceous loricate
... transport. Silicifying organisms, therefore, require an active transport mechanism to take up silicon from the external environment and to concentrate silicon within the SDV. Because silicic acid is a relatively inert species, with no known biochemical role outside that of biomineralization [4,5], s ...
... transport. Silicifying organisms, therefore, require an active transport mechanism to take up silicon from the external environment and to concentrate silicon within the SDV. Because silicic acid is a relatively inert species, with no known biochemical role outside that of biomineralization [4,5], s ...
DOCTORAL THESIS
... humans started to genetically modify plant species. Initially, humans selected more adequate variants of plants for given traits such as larger seeds for greater caloric intake, lack of bitterness, and increased yields. This process of domestication allowed the crops to adapt and eventually become l ...
... humans started to genetically modify plant species. Initially, humans selected more adequate variants of plants for given traits such as larger seeds for greater caloric intake, lack of bitterness, and increased yields. This process of domestication allowed the crops to adapt and eventually become l ...
Enzyme Redundancy and the Importance of 2
... * Corresponding author; e-mail [email protected]; fax 33– ...
... * Corresponding author; e-mail [email protected]; fax 33– ...
glucose
... of liver glycogen but not by cleavage of muscle glycogen Cleavage of glycogen in muscle and other cells provides glucose-6-P that can be metabolized only within the given cell (by glycolysis) ...
... of liver glycogen but not by cleavage of muscle glycogen Cleavage of glycogen in muscle and other cells provides glucose-6-P that can be metabolized only within the given cell (by glycolysis) ...
Recognition of Nucleic Acid Bases and Base
... Several detailed studies have analyzed the interactions observed in protein –DNA complexes, partly in efforts to determine whether a “recognition code” exists for DNA double helices.3,4,8 – 14 It seems clear that while no simple code exists, some common interaction patterns between amino acids and b ...
... Several detailed studies have analyzed the interactions observed in protein –DNA complexes, partly in efforts to determine whether a “recognition code” exists for DNA double helices.3,4,8 – 14 It seems clear that while no simple code exists, some common interaction patterns between amino acids and b ...
A minimal growth medium for the basidiomycete Pleurotus sapidus
... influence the biomass production significantly (Figure 1). After replacing the standard nutrition solution’s (SNL-H3G15) nitrogen source asparagine by ammonium nitrate (NL-H3-G15, Table 1) the growth rate was initially higher compared to SNL-H3-G15, but stalled after 48 h (Figure 2). To investigate ...
... influence the biomass production significantly (Figure 1). After replacing the standard nutrition solution’s (SNL-H3G15) nitrogen source asparagine by ammonium nitrate (NL-H3-G15, Table 1) the growth rate was initially higher compared to SNL-H3-G15, but stalled after 48 h (Figure 2). To investigate ...
SBT202 - SibTech, Inc.
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
... purification, but its existence was proved by spectral methods. The [Fe-S] clusters of NarZYV resemble by their characteristics NarGHI, except the potential of the α-subunit high-potential centers. Both enzymes catalyze nitrate and chlorate reduction and are inhibited by azide. Despite common proper ...
... purification, but its existence was proved by spectral methods. The [Fe-S] clusters of NarZYV resemble by their characteristics NarGHI, except the potential of the α-subunit high-potential centers. Both enzymes catalyze nitrate and chlorate reduction and are inhibited by azide. Despite common proper ...
CP1 Domain in Escherichia coli Leucyl
... cognate amino acids (1). The aminoacylation of tRNA is a two-step reaction: (a) activation of amino acids with ATP by forming aminoacyl adenylates and (b) transferring of the aminoacyl residue from the aminoacyl adenylate to the cognate tRNA substrate (2). The accuracy of aminoacylation depends on b ...
... cognate amino acids (1). The aminoacylation of tRNA is a two-step reaction: (a) activation of amino acids with ATP by forming aminoacyl adenylates and (b) transferring of the aminoacyl residue from the aminoacyl adenylate to the cognate tRNA substrate (2). The accuracy of aminoacylation depends on b ...
Functional analysis of hyperthermophilic endocellulase from
... found to be capable of hydrolysing cellulose at high temperatures. The hyperthermophilic cellulase has promise for applications in biomass utilization. To clarify its detailed function, we determined the crystal structures of mutants of the enzyme in complex with either the substrate or product liga ...
... found to be capable of hydrolysing cellulose at high temperatures. The hyperthermophilic cellulase has promise for applications in biomass utilization. To clarify its detailed function, we determined the crystal structures of mutants of the enzyme in complex with either the substrate or product liga ...
Dr: Mohamed I Kotb El
... Creatinine is formed from creatine and creatine phosphate in muscle and is excreted into the plasma at a constant rate related to muscle mass. Plasma creatinine is inversely related to glomerular filtration rate (GFR) and, although an imperfect measure, it is commonly used to assess glomeular fi ...
... Creatinine is formed from creatine and creatine phosphate in muscle and is excreted into the plasma at a constant rate related to muscle mass. Plasma creatinine is inversely related to glomerular filtration rate (GFR) and, although an imperfect measure, it is commonly used to assess glomeular fi ...
2006 Program
... Kendall A. Bryant, Steven H. Hinrichs, and Paul D. Fey “The characterization of the macromolecular synthesis operon of Staphylococcus epidermidis” Michael E. Olson, Luke D. Handke, Shawn R. Slater, and Paul D. Fey “The role of icaR in the regulation of and production of polysaccharide intercellular ...
... Kendall A. Bryant, Steven H. Hinrichs, and Paul D. Fey “The characterization of the macromolecular synthesis operon of Staphylococcus epidermidis” Michael E. Olson, Luke D. Handke, Shawn R. Slater, and Paul D. Fey “The role of icaR in the regulation of and production of polysaccharide intercellular ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.