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Relació estructura-funció en la família de transportadors d'aminoàcids heteromultimèrics.
Relació estructura-funció en la família de transportadors d'aminoàcids heteromultimèrics.

... amino acids Additional sodium-dependent zwitterionic transport systems of restricted distribution have been described (Table 1). Transport of L-glutamine, L-histidine, and L-asparagine in hepatocytes has been demonstrated to occur via a sodium-dependent transport system named N (276). A system with ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
Genome-wide Investigation of Cellular Functions for tRNA Nucleus

... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
E. Coli MurG: A Paradigm for a Superfamily of
E. Coli MurG: A Paradigm for a Superfamily of

... repeating β-linked disaccharide unit held together by peptide crosslinks (Fig. (1)) [2]. One of the sugars in the repeating disaccharide, N-acetyl muramic acid, is not made in eukaryotic cells. Furthermore, the peptides in the crosslinks contain both D-amino acids and unusual backbone linkages, two ...
Analysis of Honey Samples - Research Commons@Waikato
Analysis of Honey Samples - Research Commons@Waikato

... reagent for analysis and also provides Δ0 labelled internal standards for comparison. Forty eight amino acids and internal standards can be accurately detected by MRM’s in 18 minutes. Sample preparation was optimised for honey and the method was validated. The amino acid content of ten honeys were c ...
Substrate Specificity of Tonin from Rat Submaxillary Gland
Substrate Specificity of Tonin from Rat Submaxillary Gland

... same V m a l with increasing chain length while Km is steadily lowered.11 Thus the longer peptides are somewhat better substrates than the shorter ones. In a more recent study with partially purified angiotensin I-converting enzyme from pig lung, the shorter C-terminal sequences of angiotensin I wer ...
The Role of N- and C-terminal Amino Acids to
The Role of N- and C-terminal Amino Acids to

... The fundamental link between the primary amino acid sequence and the functional protein has long been an area of interest with much still to be discovered. The goal of studying protein folding is to ultimately predict the folded structure and the function of a protein from its primary sequence. Anfi ...
Thermodynamic and Biophysical Characterization of Cytochrome P450
Thermodynamic and Biophysical Characterization of Cytochrome P450

INTRODUCTION
INTRODUCTION

AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of

Organization of Physical Interactomes as
Organization of Physical Interactomes as

... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
Quantitative Analysis of the Kinetics of End
Quantitative Analysis of the Kinetics of End

CALPAIN: TRANSITIONING FROM THE USE OF THE
CALPAIN: TRANSITIONING FROM THE USE OF THE

... Calpains are a family of calcium-dependent cysteine proteases involved in intracellular signaling. They participate in many normal cellular processes such as cell motility and apoptosis but when over-activated they contribute to diseases ranging from ischemic injury to neurodegenerative disorders. T ...
Non-volatile taste components of Agaricus blazei, Antrodia
Non-volatile taste components of Agaricus blazei, Antrodia

... Cordyceps. Contents of sweet components ranged from 1.67 to 2.57 mg g 1 and were in the ascending order:
PDF - Biology Direct
PDF - Biology Direct

All fatty acids are not equal: discrimination in plant membrane lipids
All fatty acids are not equal: discrimination in plant membrane lipids

A four-column theory for the origin of the genetic code: tracing the
A four-column theory for the origin of the genetic code: tracing the

... but positive selection for the increased diversity and functionality of the proteins that can be made with a larger amino acid alphabet. Nevertheless, the code that results is one in which translational error is minimized. We define a cost function with which we can compare the fitness of codes with ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3

... of 1,524 GOs were detected in the proteome of C. coli 76339 comprising 98.8% of the complete set of tCDSs. Only 18 tCDSs did not belong to any GOs, and were unique for C. coli 76339 (Table 2). The core genome of C. coli, defined as the list of orthologs present in all C. coli strains, consisted of 6 ...
animal nutrition - e
animal nutrition - e

... Cole D J A 1994); Figure 16.4 after The Energy Metabolism of Ruminants, Hutchinson (Blaxter K L 1967) p. 259 reproduced by permission of the publisher and the author; Figure 16.7 adapted from A review of the potential of nutrition to modify milk fat and protein, Livestock Production Science 23 (3–4) ...
Iron mediated methylthiolation of tRNA as a regulator of operon
Iron mediated methylthiolation of tRNA as a regulator of operon

... 1O cell/ml for 4-5 h in 5-15 1 cultures. S30 extracts were prepared in 10 mM tris-HCl buffer (pH 7.8) , 10 mM Mg acetate,60 raM NH^Cl, 6 mM B-mercaptoethanol (14). S160 supernatants and riboscnes were obtained from S30 extracts of iron-replete organisms; ribosomes were washed twice before storing (1 ...
Dear Notetaker:
Dear Notetaker:

... o Occurs predominantly in the liver cells  Also present in kidney and maybe intestine cells  Kidney is back up plan for gluconeogenesis Enzymes of gluconeogenesis o 3 key enzymes from glycolysis that need to be bypassed in gluconeogenesis  Need to be reversed because they use ATP  Reversed with ...
General introduction - University of Amsterdam
General introduction - University of Amsterdam

... immunogenic spore formation. Sporulation might be reduced if continuous spreading from a nutrient-rich environment like the body is not necessary (48). Yeasts as model organisms Since ancient times the yeast Saccharomyces cerevisiae has been used for baking and brewing, but in the last century it wa ...
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia
UNIVERSITAT DE BARCELONA Divisió de Ciències Experimentals i Matemàtiques Facultat de Biologia

... accrue more protein —in addition to much more fat— than control reference diet-fed animals (Esteve et al., 1992), and it has been generally assumed that this is may be due in part to the higher biological value of the protein of some components of the cafeteria diets. This study has been developed a ...
The mechanism of Stx2 enrichment in outer membrane vesicles of
The mechanism of Stx2 enrichment in outer membrane vesicles of

A Metabolic Node in Action: Chorismate
A Metabolic Node in Action: Chorismate

... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
1. Introduction to Natural Products Chemistry
1. Introduction to Natural Products Chemistry

... 7. Because these compounds are produced by specific enzymes and precursors, it can be assumed that they are produced in specific parts or organelles of the plant. 8. Secondary metabolites are probably in a state of dynamic flux, being produced and broken down constantly. Some compounds, however, may ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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