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12–4 Mutations - Gravette School District
12–4 Mutations - Gravette School District

... they occur at a single point in the DNA sequence. Point mutations include substitutions, in which one base is changed to another, as well as insertions and deletions, in which a base is inserted or removed from the DNA sequence. Substitutions usually affect no more than a single amino acid. The effe ...
Brooker Chapter 13
Brooker Chapter 13

... Accelerate chemical reactions within a cell Can be divided into two main categories  Anabolic enzymes  Synthesize molecules and macromolecules  Catabolic enzymes  Break down large molecules into small ones ...
DNA markers in plant genome analysis With the advent of molecular
DNA markers in plant genome analysis With the advent of molecular

... generally classified as hybridization-based markers and polymerase chain reaction (PCR)based markers. In the former, DNA profiles are visualized by hybridizing the restriction enzyme-digested DNA, to a labelled probe, which is a DNA fragment of known origin or sequence. PCR-based markers involve in ...
Peptide synthesis – chemistry and modifications
Peptide synthesis – chemistry and modifications

... Polar mobile phases, usually water mixed with methanol, acetonitrile and/or other water miscible organic solvents, are used for elution. - Solutes are eluted in order of decreasing polarity (increasing hydrophobicity). Increasing the polar component of the mobile phase increases retention of the sol ...
Biology 9/5/12 - Scio School District Page
Biology 9/5/12 - Scio School District Page

... Checkpoint question: Characteristics of Life (example: fish)(5 min) Video: Viruses and Bacteria…10 minutes -discuss differences between viruses and bacteria Cool down question : (5 min) Viruses / Work on assignment (7 min) -students write answer…due by end of the period Assignment: Describe at least ...
Using an Alu Insertion Polymorphism to Study Human
Using an Alu Insertion Polymorphism to Study Human

... Alu is a member of the family of short interspersed elements (SINEs) and is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not prod ...
Antisense derivatives of U7 small nuclear RNA as
Antisense derivatives of U7 small nuclear RNA as

... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs (see chapter 42 Aartsma Rus). This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage tha ...
w + gene is silenced in some cells
w + gene is silenced in some cells

... • One of the few genes expressed on the inactive X but is not expressed on the active X Xist RNA is a large, non-coding, cis-acting regulatory RNA • Binds to the X-chromosome that it was expressed from ...
Lipid Synthesis
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PPT1

... • Collect all known sequences that bind a certain TF. • Align all sequences (using multiple sequence alignment). • Compute the frequency of each nucleotide in each position (PSPM). • Incorporate background frequency for each nucleotide (PSSM). ...
Phosphate stabilizing compositions
Phosphate stabilizing compositions

... For purposes of this invention, the term "polyaspartic acid" shall be construed to include salts and derivatives of polyaspartic acid. Polyaspartic acid, salts thereof, and derivatives of polyaspartic acid are well known and are described in U.S. Pat. No. 5,523,023 which is hereby incorporated by r ...
A-level Human Biology Question Paper Unit 04 - Bodies and
A-level Human Biology Question Paper Unit 04 - Bodies and

... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
An Introduction to Genetic Analysis Chapter 20 Transposable
An Introduction to Genetic Analysis Chapter 20 Transposable

... kernels from this cross were of the expected types (Figure 20-4), but one exceptional kernel was very interesting. In Figure 20-4, the first seed shows the normal solid pigment pattern owing to the presence of the dominant C allele. The second seed shows the same basic background pigmentation but wi ...
Antisense derivatives of U7 small nuclear RNA as
Antisense derivatives of U7 small nuclear RNA as

... proteins also found in spliceosomal snRNPs and two U7-specific Sm-like proteins, termed Lsm10 and Lsm11, which are essential for histone RNA processing [3, 4]. However, if the U7-specific Sm binding sequence is converted to the consensus found in spliceosomal snRNAs, a new U7 Sm OPT construct is obt ...
DIETARY FAT
DIETARY FAT

... • There are no UL’s set for Total Fat, Fatty Acids and Cholesterol because there are insufficient data to use the model of risk assessment to set a UL for total fat, monounsaturated fatty acids, and n-6 and n3 polyunsaturated fatty acids ...
Identification of Vietnamese Coptotermes pest species based on the
Identification of Vietnamese Coptotermes pest species based on the

... The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 for identification of Coptotermes species. The proposed PCR method distinguishes species by the presence or absence of DNA frag ...
amino acid mixture
amino acid mixture

... acids when presented in the free form), or by glycylglycine, which has been shown to inhibit absorption of several di- and tri-peptides in vitro (Rubino et al., 1971; Addison et al., 1974; Das & Radhakrishnan, 1975), might be explained if these amino acid residues were absorbed by an alternative pep ...
Green Fluorescent Protein: A Reporter Molecule
Green Fluorescent Protein: A Reporter Molecule

... an influx of Ca+2 causes the first protein, aequorin, to become excited and transfer the energy to the second protein, GFP, which loses the energy by emitting a photon of ...
Lecture 25 Notes
Lecture 25 Notes

... -- the chemical formula of a polyatomic acid begins with H -- the second part of the formula is a polyatomic anion containing oxygen (an oxy-anion) Rule: The name of a polyatomic acid is derived from its anion -- the ending of the anion name is modified ...
Comprehensive Analysis of Amino Acid and Nucleotide
Comprehensive Analysis of Amino Acid and Nucleotide

... against protein databases(2-6). Here, we define pseudogenes as disabled copies of genes that do not produce a functional, full-length copy of a protein (7). Operationally, these are identified as regions of the chromosome that are similar to known proteins but contain obvious disablements (such as s ...
Chapter 11 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 11 Lecture PowerPoint - McGraw Hill Higher Education

... • TAFs are not universally required for transcription of class II genes • Even TBP is not universally required • Some promoters in higher eukaryotes respond to an alternative protein such as TRF1 (TBPrelated factor 1) ...
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... Traditionally, studies concerning the transfer of information between generations have focused on DNA. As the carrier of the genetic information, DNA provides the blueprint for the next generation. However, in addition to DNA, parents transmit information to their offspring through a variety of othe ...
GFP - Bio-Rad
GFP - Bio-Rad

... settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect way to bring the excitement of biotechnology into the classroom. Each kit contains an innovative step by step protocol, which makes them the perfect choice for both experts and beginnin ...
Experimental illumination of a fitness landscape
Experimental illumination of a fitness landscape

... null-like (Fig. S2), which is likely caused by sampling noise (due to the low abundance of these codons in the competing culture). To minimize this noise, we calculated the variability among selection coefficients for synonymous codons with high fitness (s > −0.05, n = 151) as a root mean square devia ...
Ribosome Display: In Vitro Selection of Protein
Ribosome Display: In Vitro Selection of Protein

... Ribosome display is an ilt vitro technology to identify and evolve proteins or peptides binding to a given target (Fig. 1) (Hanes et nl., 2000a). While most selection technologies need living cells to achieve the essential coupling of genotype and phenotype, ribosome display uses the ribosomal compl ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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