Evolution of Enzymatic Activity in the Enolase Superfamily
... sites in highly divergent sequence contexts suggests that some OSBSs may be functionally promiscuous, that is, they catalyze another reaction as well as the OSBS reaction (10). Perhaps the extreme sequence divergence results from convergent evolution from multiple progenitors within the enolase supe ...
... sites in highly divergent sequence contexts suggests that some OSBSs may be functionally promiscuous, that is, they catalyze another reaction as well as the OSBS reaction (10). Perhaps the extreme sequence divergence results from convergent evolution from multiple progenitors within the enolase supe ...
Sequences of flavivirus-related RNA viruses persist in DNA form
... and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Aedes albopictus C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helic ...
... and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Aedes albopictus C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helic ...
How dormant origins promote complete genome replication
... licensing involves the clamping of two MCM2-7 hexamers in an antiparallel conformation around DNA [13–15]. This clamp-loading process is ATP-dependent and additionally involves proteins ORC, CDC6 and CDT1 [3,13–15]. ORC is composed of six polypeptides (ORC1–ORC6) that can bind DNA in the presence of ...
... licensing involves the clamping of two MCM2-7 hexamers in an antiparallel conformation around DNA [13–15]. This clamp-loading process is ATP-dependent and additionally involves proteins ORC, CDC6 and CDT1 [3,13–15]. ORC is composed of six polypeptides (ORC1–ORC6) that can bind DNA in the presence of ...
Is structural flexibility of antigen-binding loops
... of vibration of VH CDR3 among five clones and found that such vibration of BW9-7 was most restrained (Fig. 5). Because amino acid sequences of VH CDR3 of BW9-7 were identical to those of BW9-9, the observed limited vibration of BW9-7 VH CDR3 may be due to interaction with other loops located close t ...
... of vibration of VH CDR3 among five clones and found that such vibration of BW9-7 was most restrained (Fig. 5). Because amino acid sequences of VH CDR3 of BW9-7 were identical to those of BW9-9, the observed limited vibration of BW9-7 VH CDR3 may be due to interaction with other loops located close t ...
Protein Synthesis
... factor Tu (EF-Tu) and an aminoacyl-tRNA. The amino-terminal domain of EF-Tu is a Ploop NTPase domain similar to those in other G proteins ...
... factor Tu (EF-Tu) and an aminoacyl-tRNA. The amino-terminal domain of EF-Tu is a Ploop NTPase domain similar to those in other G proteins ...
PDF
... thermophilic bacterium, Dictyoglomus turgidum, which is a Gram negative, strictly anaerobic bacterium. D. turgidum and D. thermophilum together form the Dictyoglomi phylum. The two Dictyoglomus genomes are highly syntenic, and both are distantly related to Caldicellulosiruptor spp. D. turgidum is ab ...
... thermophilic bacterium, Dictyoglomus turgidum, which is a Gram negative, strictly anaerobic bacterium. D. turgidum and D. thermophilum together form the Dictyoglomi phylum. The two Dictyoglomus genomes are highly syntenic, and both are distantly related to Caldicellulosiruptor spp. D. turgidum is ab ...
CHAPTER 14 DNA applications in society
... the growth of all body tissues, including bones. hGH was first isolated from human pituitary glands in 1956. By the 1960s, hGH was in clinical use in the treatment of children and adolescents with a particular growth deficiency. This treatment often involved the subcutaneous injection of growth horm ...
... the growth of all body tissues, including bones. hGH was first isolated from human pituitary glands in 1956. By the 1960s, hGH was in clinical use in the treatment of children and adolescents with a particular growth deficiency. This treatment often involved the subcutaneous injection of growth horm ...
... This exam consists of 6 pages and 11 questions with 1 bonus question. Total points are 100. Allot 1 min/2 points. On questions with choices, all of your answers will be graded and the best scoring answer will be used. Please use the space provided, or the back of the preceding page. 1. (6 pts) Pleas ...
Development of a novel analytical approach combining the quantification of
... approach has been reported to be useful for the determination of the above metabolites using small sample volumes, typically microlitre volumes of a simple culture medium which lacks essential components such as amino acids. However, the scenario is completely different when analysts wish to tackle ...
... approach has been reported to be useful for the determination of the above metabolites using small sample volumes, typically microlitre volumes of a simple culture medium which lacks essential components such as amino acids. However, the scenario is completely different when analysts wish to tackle ...
16S rRNA Sequence Analysis of Bacteria Present in Foaming Activated Sludge Introduction
... Although clone 15 showed similarities of 96% and more with the organisms in Group 5, when the sequence was compared with those of other bacteria, it also indicated a 97% homology with members of Group 7 (Fig. 1). Group 7 indicated that the sequences of clones 8, 62 and 15A were more than 95% similar ...
... Although clone 15 showed similarities of 96% and more with the organisms in Group 5, when the sequence was compared with those of other bacteria, it also indicated a 97% homology with members of Group 7 (Fig. 1). Group 7 indicated that the sequences of clones 8, 62 and 15A were more than 95% similar ...
Vectors: The carriers of DNA molecules DNA vectors and their
... a heterologous source is that the gene carries no translation start signal which can be efficiently recognized by the E. coli translation system. This problem may arise for heterologous genes cloned into any host. Thus, even though the gene can be transcribed from a promoter within the vector, the r ...
... a heterologous source is that the gene carries no translation start signal which can be efficiently recognized by the E. coli translation system. This problem may arise for heterologous genes cloned into any host. Thus, even though the gene can be transcribed from a promoter within the vector, the r ...
What does PCR stand for?
... People with high levels of bitter taste receptors are less likely to smoke. ...
... People with high levels of bitter taste receptors are less likely to smoke. ...
univERsity oF copEnhAGEn
... ammonia content, indicate that amino acids are probably used as substrates during hypercapnia. When discussing the probable mechanisms involved we will make two assumptions. First, the fact that the metaDISCUSSION bolism of citric acid cycle intermediates and amino The new metabolites measured in th ...
... ammonia content, indicate that amino acids are probably used as substrates during hypercapnia. When discussing the probable mechanisms involved we will make two assumptions. First, the fact that the metaDISCUSSION bolism of citric acid cycle intermediates and amino The new metabolites measured in th ...
QUESTION - Cloudfront.net
... • ANSWER : Dna is transcibed into mRNA that leaves the nucleus and goes to the ribosome where every 3 letters are read that code for amino acids. This is translation and tRNA brings the amino acids that are put together to make a protein. Answer ...
... • ANSWER : Dna is transcibed into mRNA that leaves the nucleus and goes to the ribosome where every 3 letters are read that code for amino acids. This is translation and tRNA brings the amino acids that are put together to make a protein. Answer ...
Ch20.1 Amino-acids-degradation and synthesis
... Methionine condenses with adenosine triphosphate (ATP), forming SAM—a high-energy compound that is unusual in that it contains no phosphate. The formation of SAM is driven, in effect, by hydrolysis of all three phosphate bonds in ATP (see Figure 20.8). 2. Activated methyl group: The methyl gro ...
... Methionine condenses with adenosine triphosphate (ATP), forming SAM—a high-energy compound that is unusual in that it contains no phosphate. The formation of SAM is driven, in effect, by hydrolysis of all three phosphate bonds in ATP (see Figure 20.8). 2. Activated methyl group: The methyl gro ...
Nutrients - Saint Mary's College of California
... Digestion of Proteins • Proteases hydrolyze the peptide bond • Different proteases cleave proteins at specific positions ...
... Digestion of Proteins • Proteases hydrolyze the peptide bond • Different proteases cleave proteins at specific positions ...
3D Plus transfection reagent
... transfection. Some cell lines may behave differently and transfection efficiency can be increased without serum or under reduced serum condition. Remember that presence of serum during complex formation must be avoided. Transfection efficiency is delayed since cells have to colonize 3D matrices befo ...
... transfection. Some cell lines may behave differently and transfection efficiency can be increased without serum or under reduced serum condition. Remember that presence of serum during complex formation must be avoided. Transfection efficiency is delayed since cells have to colonize 3D matrices befo ...
INSIDER`S GUIDE Interpretation and treatment: Organic acid
... By Ron Grisanti, D.C. & Dicken Weatherby, N.D. http://www.FunctionalMedicineUniversity.com Limits of Liability & Disclaimer of Warranty We have designed this book to provide information in regard to the subject matter covered. It is made available with the understanding that the authors are not liab ...
... By Ron Grisanti, D.C. & Dicken Weatherby, N.D. http://www.FunctionalMedicineUniversity.com Limits of Liability & Disclaimer of Warranty We have designed this book to provide information in regard to the subject matter covered. It is made available with the understanding that the authors are not liab ...
Human Biology The Chemistry of Living Things 2.1 Multiple Choice
... B) Carbon atoms form four covalent bonds. C) Carbon atoms form diverse molecules that may be linear, branched, or circular. D) Carbon can form strong hydrogen bonds with other elements. E) Carbon can form bonds with hydrogen, oxygen, nitrogen, as well as another carbon atom. Answer: D ...
... B) Carbon atoms form four covalent bonds. C) Carbon atoms form diverse molecules that may be linear, branched, or circular. D) Carbon can form strong hydrogen bonds with other elements. E) Carbon can form bonds with hydrogen, oxygen, nitrogen, as well as another carbon atom. Answer: D ...
biochemistry - Textbooks Online
... If a protein solution is separated by a semipermeable membrane from pure water, water tends to flow from the latter to the former. The property of the movement of solvent particles is called as osmosis. Osmosis is the net diffusion of water from the dilute solution to the concentrated solution. Osmo ...
... If a protein solution is separated by a semipermeable membrane from pure water, water tends to flow from the latter to the former. The property of the movement of solvent particles is called as osmosis. Osmosis is the net diffusion of water from the dilute solution to the concentrated solution. Osmo ...
Biogenesis of trans-acting siRNAs, endogenous
... FRAGMENTS TO dsRNAs In general, miRNA-cleaved fragments are unstable due to the lack of polyadenylation or cap structures. In plants, the 3′ fragments generated by miRNA cleavage are promptly degraded by XRN4, which is a 5′ to 3′ exonuclease, and other unidentified RNases (Souret et al., 2004). Thus ...
... FRAGMENTS TO dsRNAs In general, miRNA-cleaved fragments are unstable due to the lack of polyadenylation or cap structures. In plants, the 3′ fragments generated by miRNA cleavage are promptly degraded by XRN4, which is a 5′ to 3′ exonuclease, and other unidentified RNases (Souret et al., 2004). Thus ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.