Streptavidin is a small bacterial protein that binds
... not lysates or cell extracts). Biotin won’t have access to protein inside the cells so only external EGFR will be biotinylated. Then, EGFR is immunoprecipitated with specific antibodies. To detect whether EGFR has been biotinylated they run the immunoprecipitates in SDS-PAGE and do Western using a p ...
... not lysates or cell extracts). Biotin won’t have access to protein inside the cells so only external EGFR will be biotinylated. Then, EGFR is immunoprecipitated with specific antibodies. To detect whether EGFR has been biotinylated they run the immunoprecipitates in SDS-PAGE and do Western using a p ...
How Biologists Classify Organisms... (pg 113
... DNA is a body’s blueprint for making proteins, so if different species make the same proteins, we can infer they carry the same “genes” that code for those proteins, and are related. 5. DNA Evidence How similar is the order of the base sequence inside DNA molecules when you compare different species ...
... DNA is a body’s blueprint for making proteins, so if different species make the same proteins, we can infer they carry the same “genes” that code for those proteins, and are related. 5. DNA Evidence How similar is the order of the base sequence inside DNA molecules when you compare different species ...
Slide 1
... affect what amino acid gets brought to the mRNA on the ribosome. • For example if the normal gene code is: ATT-GCG- AAA-CCC• If that first A is deleted, then the code would read: TTG-CGA-AAC-CC which might bring the wrong amino acid and then the wrong protein would get made which wouldn’t work prope ...
... affect what amino acid gets brought to the mRNA on the ribosome. • For example if the normal gene code is: ATT-GCG- AAA-CCC• If that first A is deleted, then the code would read: TTG-CGA-AAC-CC which might bring the wrong amino acid and then the wrong protein would get made which wouldn’t work prope ...
Chapter 17 and 19: Review Questions
... 7. Cells are able to distinguish proteins destined for secretion or compartmentation from those to be used in the cytoplasm because _____. there are two types of ribosomes: one group that synthesizes cytoplasmic ribosomes only, and another type that synthesizes compartmentalized proteins only some p ...
... 7. Cells are able to distinguish proteins destined for secretion or compartmentation from those to be used in the cytoplasm because _____. there are two types of ribosomes: one group that synthesizes cytoplasmic ribosomes only, and another type that synthesizes compartmentalized proteins only some p ...
doc - Florida State University
... 2. The backbone of each DNA strand ( not the double helix) is formed by ______ (A) the covalent bonding of base to base, (B) hydrogen bonding of base to base, (C) hydrogen bonding of amino group to the sugar, (D) hydrogen bonding of sugar to base, (E) covalent bonding of sugar to sugar 3. DNA consis ...
... 2. The backbone of each DNA strand ( not the double helix) is formed by ______ (A) the covalent bonding of base to base, (B) hydrogen bonding of base to base, (C) hydrogen bonding of amino group to the sugar, (D) hydrogen bonding of sugar to base, (E) covalent bonding of sugar to sugar 3. DNA consis ...
doc - Florida State University
... operator, (B) result in a decrease in the transcription of structural genes controlled by the LAC operon, (C) make the repressor protein capable of binding to the operator, (D) A and B but not C OR (E) B and C but not A. 34. The role of the polyA tail in mRNA is to _____ (A) facilitate binding of th ...
... operator, (B) result in a decrease in the transcription of structural genes controlled by the LAC operon, (C) make the repressor protein capable of binding to the operator, (D) A and B but not C OR (E) B and C but not A. 34. The role of the polyA tail in mRNA is to _____ (A) facilitate binding of th ...
CS691K Bioinformatics Kulp Lecture Notes #0 Molecular
... Regular, long, stable, oriented, double-stranded, helical structure Humans: 23 pairs of chromosomes. Total ~3B “bases” (x2) DNA resides in nucleus in eukaryotes ...
... Regular, long, stable, oriented, double-stranded, helical structure Humans: 23 pairs of chromosomes. Total ~3B “bases” (x2) DNA resides in nucleus in eukaryotes ...
Chapter 6 - Education Reformation
... As a result of this polarity: *Water can dissolve many ionic compounds. Ex. Salt and Sugar **Water molecules attract other water molecules, forming a Hydrogen Bond. This gives Water great stability. ***Hydrogen Bonds Also helps hold together many large molecules, such as proteins and DNA. ...
... As a result of this polarity: *Water can dissolve many ionic compounds. Ex. Salt and Sugar **Water molecules attract other water molecules, forming a Hydrogen Bond. This gives Water great stability. ***Hydrogen Bonds Also helps hold together many large molecules, such as proteins and DNA. ...
molecular scissors to study gene function Marta Oliveira
... interspaced by unique DNA regions that come from several bacterial viruses, which works as a DNA record of previous attacks. It was later proved that the CRISPR loci, together with many enzymes, were in fact a bacterial defense mechanism against life threatening viral attacks, preventing the virus f ...
... interspaced by unique DNA regions that come from several bacterial viruses, which works as a DNA record of previous attacks. It was later proved that the CRISPR loci, together with many enzymes, were in fact a bacterial defense mechanism against life threatening viral attacks, preventing the virus f ...
Deamination of 5-methylcytosine yields thymine
... the rate of cell proliferation in vitro? Thymidine will be incorporated into replicating DNA but not RNA. 2. Provide a biochemical rationale for why AT-rich sequences are commonly found in zones of initiation of DNA replication and near sites of transcriptional termination. Only two hydrogen bonds n ...
... the rate of cell proliferation in vitro? Thymidine will be incorporated into replicating DNA but not RNA. 2. Provide a biochemical rationale for why AT-rich sequences are commonly found in zones of initiation of DNA replication and near sites of transcriptional termination. Only two hydrogen bonds n ...
DNA makes up chromosomes!
... 1. Enzymes unzips DNA by breaking hydrogen bonds. Strands separate at the replication fork. ...
... 1. Enzymes unzips DNA by breaking hydrogen bonds. Strands separate at the replication fork. ...
Biological Macromolecules
... **Antibodies are part of the immune system. **When something enters the body that isn’t supposed to be there, like certain bacteria, antibodies find the invader and stick themselves onto it. **White Blood cells destroy the invaders (hopefully) ...
... **Antibodies are part of the immune system. **When something enters the body that isn’t supposed to be there, like certain bacteria, antibodies find the invader and stick themselves onto it. **White Blood cells destroy the invaders (hopefully) ...
Cell Division and Mitosis
... alternating sets of phosphate and sugar molecules. One side is 5’-3’ and the other side is 3’ - 5’. Base pairs make up the rungs of the ladder A—T and G– C. The genetic code is arranged as sets of three base codes together. For example AAA, CGC, ATC, CCC, would all code for four different amino ...
... alternating sets of phosphate and sugar molecules. One side is 5’-3’ and the other side is 3’ - 5’. Base pairs make up the rungs of the ladder A—T and G– C. The genetic code is arranged as sets of three base codes together. For example AAA, CGC, ATC, CCC, would all code for four different amino ...
Chromosomes, genes, alleles, and mutation
... • Differ from other alleles by only one or a few bases • Alleles of the same gene occupy a corresponding place (locus) on each chromosome of a pair ...
... • Differ from other alleles by only one or a few bases • Alleles of the same gene occupy a corresponding place (locus) on each chromosome of a pair ...
Chapters 13-16, Molecular Genetics
... c. three base code (43) = a three base code will produce 64 possibilities, more than enough 2. codon = 3 bases found on mRNA a. 3 “stop” codons b. 1 “start” codon c. third base in the codon is often less specific than the first two d. several codons can code for the same amino acid (degenerate) 3. g ...
... c. three base code (43) = a three base code will produce 64 possibilities, more than enough 2. codon = 3 bases found on mRNA a. 3 “stop” codons b. 1 “start” codon c. third base in the codon is often less specific than the first two d. several codons can code for the same amino acid (degenerate) 3. g ...
Nature vs. Nurture
... • Turns genes off by causing DNA to wrap more tightly around histone so RNA polymerase cannot get to the gene DNA sequence ...
... • Turns genes off by causing DNA to wrap more tightly around histone so RNA polymerase cannot get to the gene DNA sequence ...
Control of gene expression in prokaryotes and eukaryotes
... Gene expression is transcription of DNA to make RNA and then using the RNA to make proteins. This process can’t be left on indefinitely. The turning on and off of genes is critical to the development of an organism and the organism functioning properly throughout its life. Eukaryotic control Pretran ...
... Gene expression is transcription of DNA to make RNA and then using the RNA to make proteins. This process can’t be left on indefinitely. The turning on and off of genes is critical to the development of an organism and the organism functioning properly throughout its life. Eukaryotic control Pretran ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.