Jigsaw handout - the Biology Scholars Program Wiki
... repressor. Lac repressor is a DNA binding protein that binds to a site on the DNA called the operator. The operator partially overlaps with the promoter, which is the site where RNA polymerase binds to initiate transcription. When lac repressor binds to the operator site, RNA polymerase cannot bind ...
... repressor. Lac repressor is a DNA binding protein that binds to a site on the DNA called the operator. The operator partially overlaps with the promoter, which is the site where RNA polymerase binds to initiate transcription. When lac repressor binds to the operator site, RNA polymerase cannot bind ...
The coiled-coil of the human Rad50 DNA repair protein contains
... globular ATPase domain made up from the N and C termini of the protein separated by an extensive central region predicted to form a coiled-coil (2). Accumulated recent evidence reveals that Rad50 and other SMC proteins are arranged as intramolecular coiled-coils bringing together the N and C termini ...
... globular ATPase domain made up from the N and C termini of the protein separated by an extensive central region predicted to form a coiled-coil (2). Accumulated recent evidence reveals that Rad50 and other SMC proteins are arranged as intramolecular coiled-coils bringing together the N and C termini ...
Engineering a tRNA and aminoacyl-tRNA synthetase for the site
... Choice of tRNA-Aminoacyl Synthetase System. The original strategy that was used to generate an orthogonal tRNA for our in vitro mutagenesis methodology involved the use of the yeast phenylalanyl suppressor tRNA, which was known not to be a substrate for any E. coli aminoacyl-tRNA synthetase (17). On ...
... Choice of tRNA-Aminoacyl Synthetase System. The original strategy that was used to generate an orthogonal tRNA for our in vitro mutagenesis methodology involved the use of the yeast phenylalanyl suppressor tRNA, which was known not to be a substrate for any E. coli aminoacyl-tRNA synthetase (17). On ...
VCE Biology TSFX REVISION LECTURE UNIT 3 Part 1
... and tertiary (overall chain shape) structures, and that those proteins made up of two or more polypeptide chains may be described by a quaternary structure. Students are expected to identify α-helices and β-pleated sheets as being the most common secondary structures. They are expected to understand ...
... and tertiary (overall chain shape) structures, and that those proteins made up of two or more polypeptide chains may be described by a quaternary structure. Students are expected to identify α-helices and β-pleated sheets as being the most common secondary structures. They are expected to understand ...
Determination of free amino acids in cheeses from the Czech market
... amino acids in cheeses from the Czech market. Czech J. Food Sci., 27: 143–150. High performance liquid chromatography (HPLC) method with the pre-column derivatisation by AccQ•Tag agent and following determination of these derivates after their separation in reverse phase column followed by fluoresce ...
... amino acids in cheeses from the Czech market. Czech J. Food Sci., 27: 143–150. High performance liquid chromatography (HPLC) method with the pre-column derivatisation by AccQ•Tag agent and following determination of these derivates after their separation in reverse phase column followed by fluoresce ...
in plant physiology
... Most NEP promoters (rpoB, rpoA, and accD) share a core sequence, the YRTA motif (type-Ia) (Liere and Maliga, 1999; Hirata et al., 2004; Weihe and Borner, 1999) (Fig. 1A). The YRTA motif is similar to motifs found in promoters of plant mitochondria (Binder and Brennicke, 2003; Kuhn et al., 2005). In ...
... Most NEP promoters (rpoB, rpoA, and accD) share a core sequence, the YRTA motif (type-Ia) (Liere and Maliga, 1999; Hirata et al., 2004; Weihe and Borner, 1999) (Fig. 1A). The YRTA motif is similar to motifs found in promoters of plant mitochondria (Binder and Brennicke, 2003; Kuhn et al., 2005). In ...
Lecture 9: Citric Acid Cycle/Fatty Acid Catabolism
... released as CO2, i.e., that from oxaloacetate. This was interpreted as proof that citrate is not in the cycle at all the labels would have been scrambled, and half of the CO2 would have been 14C. Prochiral Citrate. In a two-minute thought experiment, Alexander Ogston in 1948 (Nature, 162: 963) argue ...
... released as CO2, i.e., that from oxaloacetate. This was interpreted as proof that citrate is not in the cycle at all the labels would have been scrambled, and half of the CO2 would have been 14C. Prochiral Citrate. In a two-minute thought experiment, Alexander Ogston in 1948 (Nature, 162: 963) argue ...
the significance of hydrogen bonding
... a cleavage or an isomerization of a phosphodiester bond. Numerous agents may catalyze the cleavage of RNA, including specific acids and bases, metal ions and their complexes not to mention naturally occurring enzymes.2-4 Nature has optimized the structure of the active sites of RNA-cleaving enzymes ...
... a cleavage or an isomerization of a phosphodiester bond. Numerous agents may catalyze the cleavage of RNA, including specific acids and bases, metal ions and their complexes not to mention naturally occurring enzymes.2-4 Nature has optimized the structure of the active sites of RNA-cleaving enzymes ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
The Family of SMF Metal Ion Transporters in Yeast Cells*
... peptidase under limited manganese concentrations in the medium (8). Further studies indicated the SMF1 is a general metal ion transporter and can transport not only Mn2⫹, Zn2⫹, and Cu2⫹ (8), but also Fe2⫹, Cd2⫹, Ni2⫹, and Co2⫹ (9, 12, 14). Yeast cells contain additional two genes of this family, SMF ...
... peptidase under limited manganese concentrations in the medium (8). Further studies indicated the SMF1 is a general metal ion transporter and can transport not only Mn2⫹, Zn2⫹, and Cu2⫹ (8), but also Fe2⫹, Cd2⫹, Ni2⫹, and Co2⫹ (9, 12, 14). Yeast cells contain additional two genes of this family, SMF ...
amino-acids - ChemConnections
... The enantiomer that rotates the plane of polarized light to the left is called L- (laevus = “left”) and the other enantiomer is called D- (dexter = right). Enantiomers have identical physical and chemical properties. They only differ in their interaction with other enantiomers. Most amino acids in p ...
... The enantiomer that rotates the plane of polarized light to the left is called L- (laevus = “left”) and the other enantiomer is called D- (dexter = right). Enantiomers have identical physical and chemical properties. They only differ in their interaction with other enantiomers. Most amino acids in p ...
Text S1.
... sequences show substantially higher ratios of non-synonymous over synonymous nucleotide differences (KA/KS = 0.91 for AlSRK06 and AhSRK23, and KA/KS = 1.13 for AlSRK04 and AhSRK20) than the sequences from the set of trans-specifically shared S-alleles (average KA/KS = 0.29). This is likely to be due ...
... sequences show substantially higher ratios of non-synonymous over synonymous nucleotide differences (KA/KS = 0.91 for AlSRK06 and AhSRK23, and KA/KS = 1.13 for AlSRK04 and AhSRK20) than the sequences from the set of trans-specifically shared S-alleles (average KA/KS = 0.29). This is likely to be due ...
Lecture 9 Fatty Acid Synthesis
... Uses NADPH as reductant Lengthens a fatty acid by using malonyl CoA as acetyl donor Fatty acid lengthens by 2 carbons Brain-Elongation capibilities-Very long chain fatty acids(24 C) – Synthesis of Brain lipids ...
... Uses NADPH as reductant Lengthens a fatty acid by using malonyl CoA as acetyl donor Fatty acid lengthens by 2 carbons Brain-Elongation capibilities-Very long chain fatty acids(24 C) – Synthesis of Brain lipids ...
THE EFFECT OF ISOELECTRIC AMINO ACIDS ON THE pH + OF A
... Two amino acids were employed, glycine and alanine. They were purified by recrystallization at their isoelectric points; and their purity was established by determinations of the relative and absolute values of total and free amino nitrogen. Solutions of these amino acids were made with ionic streng ...
... Two amino acids were employed, glycine and alanine. They were purified by recrystallization at their isoelectric points; and their purity was established by determinations of the relative and absolute values of total and free amino nitrogen. Solutions of these amino acids were made with ionic streng ...
Changes to Exempt Dealings - Office of the Gene Technology
... No. If your NLRD was notified prior to 31 March 2007, the NLRD authority remains and you do not need to apply again if it is now reclassified as a ‘PC1’ NLRD. However, if you have (or will commence work on) a dealing that was classified as an exempt dealing after 31 March 2007, you DO need to obtain ...
... No. If your NLRD was notified prior to 31 March 2007, the NLRD authority remains and you do not need to apply again if it is now reclassified as a ‘PC1’ NLRD. However, if you have (or will commence work on) a dealing that was classified as an exempt dealing after 31 March 2007, you DO need to obtain ...
19.1 CONSTITUTIVE, INDUCIBLE AND REPRESSIBLE GENE
... Lac gene regulation is a classic case because the Operon Model was the first example of the molecular basis for environment-genome interaction. Substrate recognition and catalysis by E. coli β-galactosidase does not depend on recognition of the glucose moiety of lactose. In fact, β-galactosidase fro ...
... Lac gene regulation is a classic case because the Operon Model was the first example of the molecular basis for environment-genome interaction. Substrate recognition and catalysis by E. coli β-galactosidase does not depend on recognition of the glucose moiety of lactose. In fact, β-galactosidase fro ...
Effects of Genic Base Composition on Growth Rate in G+C
... genomes, the input of new mutations would not produce the observed base compositions. In general, new mutations would almost universally result in genomes that are more A+T-rich. The disparity between the G+C content expected from new mutations to a genome and its current base composition is best ex ...
... genomes, the input of new mutations would not produce the observed base compositions. In general, new mutations would almost universally result in genomes that are more A+T-rich. The disparity between the G+C content expected from new mutations to a genome and its current base composition is best ex ...
Construction of plant BAC libraries This document
... 3. Place assembly joints, glass tubes, collection cups, and the cuvette rack on the Q-tray cover. Add 1X TAE to each of the three assembly joint/collection cup combinations until a reverse meniscus is visible at the top of each assembly joint. Gently place a piece of sterile Miracloth onto the rever ...
... 3. Place assembly joints, glass tubes, collection cups, and the cuvette rack on the Q-tray cover. Add 1X TAE to each of the three assembly joint/collection cup combinations until a reverse meniscus is visible at the top of each assembly joint. Gently place a piece of sterile Miracloth onto the rever ...
Intro to Cell Biology
... (SUGARS attached to ________) on the surface of cells help cells ____________ More about this in Chapters 7 and 11 Image from: http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio%20101/Bio%20101%20Lectures/Membranes/membrane.htm ...
... (SUGARS attached to ________) on the surface of cells help cells ____________ More about this in Chapters 7 and 11 Image from: http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio%20101/Bio%20101%20Lectures/Membranes/membrane.htm ...
Nucleotide Sequence of the Gene Coding for the
... not conserved, and are these regions responsible for the obN2 3H2+ 2NH3; the detailsof its mechanism are notknown served differences in the nitrogenase proteins? If this is true, (1). The enzyme system has been isolated from at least 10 may it be possible, by DNA recombination in vitro, to condiffer ...
... not conserved, and are these regions responsible for the obN2 3H2+ 2NH3; the detailsof its mechanism are notknown served differences in the nitrogenase proteins? If this is true, (1). The enzyme system has been isolated from at least 10 may it be possible, by DNA recombination in vitro, to condiffer ...
Gene Section FANCF (Fanconi anemia, complementation group F)
... FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FAN ...
... FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FAN ...
Trilateral Project WM4 Report on comparative study on Examination
... challenge because it is doubtful that the claimed polynucleotides would be able to detect differences between haplotypes. Each polynucleotide is 3,267 nucleotides in length and too long to specifically hybridise to particular haplotypes. If it is claimed that the response of patients with disease X ...
... challenge because it is doubtful that the claimed polynucleotides would be able to detect differences between haplotypes. Each polynucleotide is 3,267 nucleotides in length and too long to specifically hybridise to particular haplotypes. If it is claimed that the response of patients with disease X ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.