Amplification of 16S rRNA Genes from Frankia Strains in Root
... 1, plant associated with the indigenous flora of New Zealand; 2, introduced species. ...
... 1, plant associated with the indigenous flora of New Zealand; 2, introduced species. ...
GeorgeGamow-Wikipedi..
... In the early 1900s, radioactive materials were known to have characteristic exponential decay rates or half lives. At the same time, radiation emissions were known to have certain characteristic energies. By 1928, Gamow had solved the theory of the alpha decay of a nucleus via tunnelling, with mathe ...
... In the early 1900s, radioactive materials were known to have characteristic exponential decay rates or half lives. At the same time, radiation emissions were known to have certain characteristic energies. By 1928, Gamow had solved the theory of the alpha decay of a nucleus via tunnelling, with mathe ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
peptide guideline-Application notes.ai
... Lyophilized peptides should be refrigerated and stored away from bright light. For short-term storage ranging from one week to two months, lyophilized peptides may be stored at 4°C. For long-term storage, lyophilized peptide should be stored at -20°C in deep freezer. Even though this condition keeps ...
... Lyophilized peptides should be refrigerated and stored away from bright light. For short-term storage ranging from one week to two months, lyophilized peptides may be stored at 4°C. For long-term storage, lyophilized peptide should be stored at -20°C in deep freezer. Even though this condition keeps ...
Computational Biology - Bioinformatik
... SNPs in miRNA may lead to diseases miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cance ...
... SNPs in miRNA may lead to diseases miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cance ...
Supplementary Information (doc 224K)
... Online supplementary information (SI) Materials and methods Isolation of DNA A number of commercially available kits are known to provide adequate yield and quality of DNA from peripheral blood specimens including, for example, the QIAmp DNA blood (Mini) Kit (Qiagen, Hilden, Germany). The extraction ...
... Online supplementary information (SI) Materials and methods Isolation of DNA A number of commercially available kits are known to provide adequate yield and quality of DNA from peripheral blood specimens including, for example, the QIAmp DNA blood (Mini) Kit (Qiagen, Hilden, Germany). The extraction ...
Origins and Early Evolution of the tRNA Molecule
... non-coded protein synthesis based on the ligation of short peptides could have existed earlier. ...
... non-coded protein synthesis based on the ligation of short peptides could have existed earlier. ...
TD12 Characterization of DnaJ substrate specificity Reference
... -white bar = DnaK binding peptides from a previous study -black bar = DnaJ binding peptide -bars that go above the mid-line indicate the DnaJ binding peptides were enriched in that amino acid, bars below indicate that amino acid is depleted -DnaJ binders are enriched in aromatics (F,W,Y) and hydroph ...
... -white bar = DnaK binding peptides from a previous study -black bar = DnaJ binding peptide -bars that go above the mid-line indicate the DnaJ binding peptides were enriched in that amino acid, bars below indicate that amino acid is depleted -DnaJ binders are enriched in aromatics (F,W,Y) and hydroph ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Molecular evidence for the existence of additional members of the
... tract infections. The use of broad range bacterial primers and nucleotide sequencing for identification is only useful when one species is expected (Goldenberger et al., 1997; Greisen et al., 1994). The presence of more than one species would necessitate lengthy cloning and sequencing procedures. Th ...
... tract infections. The use of broad range bacterial primers and nucleotide sequencing for identification is only useful when one species is expected (Goldenberger et al., 1997; Greisen et al., 1994). The presence of more than one species would necessitate lengthy cloning and sequencing procedures. Th ...
A Study of Free Amino Acids and of Glutamine
... of liver and muscle, the free a-amino nitrogen of these tissues was also determined. In the liver (Table 2), no significant difference in the total free amino acids between the tumor-bearing and con trol animals was ever found. Yet, a definite in crease in the concentrations of aspartic acid, serine ...
... of liver and muscle, the free a-amino nitrogen of these tissues was also determined. In the liver (Table 2), no significant difference in the total free amino acids between the tumor-bearing and con trol animals was ever found. Yet, a definite in crease in the concentrations of aspartic acid, serine ...
Protein content and amino acids profile of
... comparable to whole-grain wheat (Triticum spp.) (13.2%). These pseudocereals contain minor protein content when compared with legume seeds such as bean (Phaseolus vulgaris) with 23.6% or soya (Glycine max) with 36.1% (USDA, 2011). The most important aspect of a protein, from a nutritional point of v ...
... comparable to whole-grain wheat (Triticum spp.) (13.2%). These pseudocereals contain minor protein content when compared with legume seeds such as bean (Phaseolus vulgaris) with 23.6% or soya (Glycine max) with 36.1% (USDA, 2011). The most important aspect of a protein, from a nutritional point of v ...
Expanding the Genetic Code
... The genetic codes of all known organisms specify the same 20 amino acid building blocks. These building blocks contain a limited number of functional groups including carboxylic acids and amides, a thiol and thiol ether, alcohols, basic amines, and alkyl and aryl groups. Although various arguments h ...
... The genetic codes of all known organisms specify the same 20 amino acid building blocks. These building blocks contain a limited number of functional groups including carboxylic acids and amides, a thiol and thiol ether, alcohols, basic amines, and alkyl and aryl groups. Although various arguments h ...
The final publication is available at Copyright - RiuNet
... main characteristic of Stevia leaves is high sweetness (250-300 times sweeter than sucrose) due to the ...
... main characteristic of Stevia leaves is high sweetness (250-300 times sweeter than sucrose) due to the ...
Chapter 23
... metabolized into succinyl CoA. In this pathway, propionyl-CoA is converted to methylmalonylCoA, a process driven in part by ATP hydrolysis. Succinyl-CoA is a citric acid cycle intermediate that is metabolized to oxaloacetate. This process yields 1 GTP, one FAD-dependent oxidation, and one NAD+-depen ...
... metabolized into succinyl CoA. In this pathway, propionyl-CoA is converted to methylmalonylCoA, a process driven in part by ATP hydrolysis. Succinyl-CoA is a citric acid cycle intermediate that is metabolized to oxaloacetate. This process yields 1 GTP, one FAD-dependent oxidation, and one NAD+-depen ...
Polypeptides, Nucleic Acid Molecules, and Methods
... This invention was made with government support under Grant No. CBET-0828817 awarded by the National Science Foundation and under grant number 2P20 RR020171 from the National Center for Research Resources of the National Institutes of Health. The government has certain rights in the ...
... This invention was made with government support under Grant No. CBET-0828817 awarded by the National Science Foundation and under grant number 2P20 RR020171 from the National Center for Research Resources of the National Institutes of Health. The government has certain rights in the ...
In vivo evidence for the prokaryotic model of extended codon
... (lane 1), a well-de®ned set of proteins is labeled in WT cells, including cyt f, as evidenced by the speci®c absence of this species in strain DpetA, in which the petA gene is deleted (lane 2). Apart from these controls, the results showed that the ectopic expression of either the WT-A37, U37 or G37 ...
... (lane 1), a well-de®ned set of proteins is labeled in WT cells, including cyt f, as evidenced by the speci®c absence of this species in strain DpetA, in which the petA gene is deleted (lane 2). Apart from these controls, the results showed that the ectopic expression of either the WT-A37, U37 or G37 ...
paper - Université de Namur
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
2004-011: Draft Annex to ISPM 27:2006 – Xanthomonas citri subsp
... multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls as are used for enzyme-linked immunosorbent assay (ELISA). Commercially available antiserum is diluted with PBS ( ...
... multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls as are used for enzyme-linked immunosorbent assay (ELISA). Commercially available antiserum is diluted with PBS ( ...
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
... also exist within bacterial entomopathogen species and may have different pathogenic or virulence levels to the insect host. Therefore, rapid and accurate detection of bacteria is needed to differentiate them from others according to their role and to potentially use them as biological control. It h ...
... also exist within bacterial entomopathogen species and may have different pathogenic or virulence levels to the insect host. Therefore, rapid and accurate detection of bacteria is needed to differentiate them from others according to their role and to potentially use them as biological control. It h ...
Pseudouridine at position 55 in tRNA controls the contents of other
... 15 years since the identification of the truB gene. In vivo functional study of the 55 modification in tRNA is not straightforward because a truB deletion mutant generally grows normally. For example, deletion of E. coli truB does not affect exponential growth (37) and the lack of the 55 modification ...
... 15 years since the identification of the truB gene. In vivo functional study of the 55 modification in tRNA is not straightforward because a truB deletion mutant generally grows normally. For example, deletion of E. coli truB does not affect exponential growth (37) and the lack of the 55 modification ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... Growth Factor—1 from Its Receptor—bdining Activities by Site—directed Mutagenesis of a Single Lysine Residue” Journal of Cell Biology 111:2129—2138. ...
... Growth Factor—1 from Its Receptor—bdining Activities by Site—directed Mutagenesis of a Single Lysine Residue” Journal of Cell Biology 111:2129—2138. ...
Unusual dehydrations in anaerobic bacteria
... was reported in 1965 [27]. Due to the complicated, coupled assay it is not clear, however, whether the hydration of acryloyl-CoA to lactyiCoA was actually measured. Furthermore, the activity was observed under aerobic conditions under which lactyl-CoA dehydratase from C. propionicum [8,14] was immed ...
... was reported in 1965 [27]. Due to the complicated, coupled assay it is not clear, however, whether the hydration of acryloyl-CoA to lactyiCoA was actually measured. Furthermore, the activity was observed under aerobic conditions under which lactyl-CoA dehydratase from C. propionicum [8,14] was immed ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.