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Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... methylation pattern acquired in response to abiotic or biotic stress is often inherited over one to several subsequent generations. Cytosine methylation marks affect physiological functions of plants via their effect(s) on gene expression levels. They also repress transposable elements that are abun ...
Determination of Nutrient Contents and Amino acid Composition of
Determination of Nutrient Contents and Amino acid Composition of

... calcium, zinc and total protein with values: 0.01±0.001 mg/100 ml, 5.78±0.015 mg/100 ml, 2.26±0.020 mg/100 ml, 0.41±0.010 mg/100 ml, 5.524±0.020 g/100 ml, respectively. This extract was found contained at least 17 amino acids. The major amino acids are glutamat, lysine, leucine, asparagin, alanin, a ...
fulltext
fulltext

... Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydrated and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tard ...
Supplementary Materials and methods (doc 154K)
Supplementary Materials and methods (doc 154K)

... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
KIEBER, ROBERT J., LINDA H. HYDRO, AND PAMELA J. SEATON
KIEBER, ROBERT J., LINDA H. HYDRO, AND PAMELA J. SEATON

Pentose phosphates in nucleoside interconversion and catabolism
Pentose phosphates in nucleoside interconversion and catabolism

... phosphorylase activity, therefore they cannot carry out these kinds of Rib-1-P recycling. N-deoxyribosyltransferases Contrary to the ribose moiety of inosine, which must be transformed by PNP into free Rib-1-P in order to be transferred to a nucleobase, the deoxyribose moiety of deoxyinosine can be ...
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology

... The three isolates were tested for their capacity to grow on organic matter released by a M. capsulatus culture. A cell-free supernatant fraction from a pure continuous methane-grown culture of M. capsulatus was incubated for 48 h at 45°C. Afterwards, the fraction contained approximately half of the ...
Seminario Glúcidos 3 y lípidos 1. Comente los mecanismos de
Seminario Glúcidos 3 y lípidos 1. Comente los mecanismos de

... Schneider, and Pallade (5, 6) was used for the preparation of the particulate fractions of rat liver. Normal adult albino rats of Sprague-Dawley stock were used throughout this study. The animals were killed by decapitation and exsanguinated. The livers were quickly removed and chilled in cracked ic ...
15. The Importance of Energy Changes and Electron Transfer in
15. The Importance of Energy Changes and Electron Transfer in

The Minimal Nutritional Requirements of Organisms
The Minimal Nutritional Requirements of Organisms

Triacylglycerol Metabolism Gone Bad: A major cause of disease
Triacylglycerol Metabolism Gone Bad: A major cause of disease

... – Increase synthesis of muscle and liver genes involved in fatty acid uptake and oxidation. ...
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE

... Most of the bases in the genome consist of “junk DNA” with no known function. The chromosomes differ in the number of genes they carry. ...
Article Are Convergent and Parallel Amino Acid Substitutions in
Article Are Convergent and Parallel Amino Acid Substitutions in

... same evolutionary path. We excluded connected branch pairs because of the difficulty in inferring molecular convergence in these branch pairs. For example, parallel substitutions in the exterior branches respectively leading to D. yakuba and D. erecta will almost always be inferred as a substitution ...
Lecture 008, Tissue - SuperPage for Joel R. Gober, PhD.
Lecture 008, Tissue - SuperPage for Joel R. Gober, PhD.

... >> Yeah, sure. Because that’s why it turned in to Carbon Dioxide and water. Yeah, by itself it will do it how? It will do it very slowly. Okay? Because there’s an activation energy barrier but if we put in the energy to that dust we can get all of these dust to turn into carbon dioxide and water ver ...
Rabbit Reticulocyte Lysate Technical Manual
Rabbit Reticulocyte Lysate Technical Manual

... characterize mRNA populations rapidly. Two basic types of system are available: 1) translation systems for RNA templates, and 2) coupled transcription/translation systems for DNA templates. Many factors influence the choice of an in vitro translation system; some of these considerations are summariz ...
PDF - MD Body and Med spa
PDF - MD Body and Med spa

... When beta-oxidation outstrips carbohydrate breakdown (e.g. during starvation and uncontrolled diabetes) ketone bodies are produced by liver mitochondria. This is because the large amount of acetyl CoA produced by beta oxidation overloads the citric acid cycle (limited carbohydrate means that citric ...
FLUORESCENT PROTEIN IN Escherichia coli
FLUORESCENT PROTEIN IN Escherichia coli

The Metabolism of Triglycerides by Spores of Penic
The Metabolism of Triglycerides by Spores of Penic

... 650 mp which corresponded to about 5 x lo8 spores/ml. as determined from a Calibration curve of haemocytometer counts of spores against extinction. Samples of these spore suspensions (10 ml.) were incubated at 30°, the optimal temperature for oxidation, with equimolecular amounts of triglycerides (n ...
Chapter 2 Chemistry of Life - Cardinal Newman High School
Chapter 2 Chemistry of Life - Cardinal Newman High School

... Copyright © 2007, 2005, 2002, 1997, 1992 by Mosby, Inc., an affiliate of Elsevier Inc. All rights reserved. ...
Biochemical studies on a versatile esterase that is most catalytically
Biochemical studies on a versatile esterase that is most catalytically

... protein at 40°C and pH 8.0). The enzyme also accepts 44 structurally different common esters with different levels of enantio-selectivity (1.0–55 000 units g−1 protein), including (±)-menthyl-acetate, (±)-neomenthyl acetate, (±)-pantolactone, (±)-methyl-mandelate, (±)methyl-lactate and (±)-glycidyl ...
Mechanisms of Transcription-Replication
Mechanisms of Transcription-Replication

... plasmid carrying the lacIq gene (Fig. 4C), it was completely repressed in the absence of IPTG, while being strongly activated in its presence (20). Figure 4C shows that replication stalling in this case was evident only when transcription from the head-on–oriented trc promoter was active. We have al ...
to this tutorial as a PDF
to this tutorial as a PDF

amino acids and proteins
amino acids and proteins

... • In histidine, the imidazole side chain also contains titratable group. • At very low pH values, histidine has a net positive charge of 2 as both the imidazole and amino groups bear positive charges. • As base is added, the pH increases and the carboxyl group loses a proton to become a carboxylate ...
BI 112 Instructor: Waite Exam #4 Study Guide Cell Membrane
BI 112 Instructor: Waite Exam #4 Study Guide Cell Membrane

... enzyme complex involved in transcription is called RNA polymerase ...
Amino acid transport in Penicillium chrysogenum in relation to
Amino acid transport in Penicillium chrysogenum in relation to

... ACVS has been a matter of debate for some time. Initially, it was described as a membrane associated protein and found to co-sediment with vesicles of either Golgi or vacuolar origin (52, 106, 111). However, the amino acid sequence of P. chrysogenum ACV synthetase contains no recognizable targeting ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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